中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5221-5224.doi: 10.3969/j.issn.1673-8225.2011.28.023

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

血管内皮生长因子及其受体在大鼠附睾内精子上的表达

艾庆燕，赵豫凤，王艳梅，苗乃周，马  莉，杨加周   

  1. 延安大学医学院组织胚胎学教研室，陕西省延安市  716000
  • 收稿日期:2011-02-14 修回日期:2011-04-16 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 杨加周，副教授，延安大学医学院组织胚胎学教研室，陕西省延安市 716000
  • 作者简介:艾庆燕★，女，1975年生，陕西省延安市人，汉族，2008年延安大学毕业，硕士，讲师，主要从事生殖医学研究。
  • 基金资助:

    陕西省科技厅自然科学基础项目(2010JM4024)；陕西省教育厅专项科研计划项目(2010JK906，2010JK907)；延安市科学技术研究发展计划项目(2009KS-12)；延安大学科研计划项目(YD2008-04)。

Expression of vascular endothelial growth factor and its receptor in the epididymal sperm of adolescent male rats

Ai Qing-yan, Zhao Yu-feng, Wang Yan-mei, Miao Nai-zhou, Ma Li, Yang Jia-zhou   

  1. Department of Human Histology and Embryology, Medical School of Yan’an University, Yan’an  716000, Shaanxi Province, China
  • Received:2011-02-14 Revised:2011-04-16 Online:2011-07-09 Published:2011-07-09
  • Contact: Yang Jia-zhou, Associate professor, Department of Human Histology and Embryology, Medical School of Yan’an University, Yan’an 716000, Shaanxi Province, China jiazhouy@163.com
  • About author:Ai Qing-yan★, Master, Lecturer, Department of Human Histology and Embryology, Medical School of Yan’an University, Yan’an 716000, Shaanxi Province, China aqy_2001@163.com
  • Supported by:

    the Natural Science Foundation of Shaanxi Science and Technology Bureau, No. 2010JM4024*; Specific Science and Technology Research Plan of Shaanxi Education Bureau, No. 2010JK906*, 2010JK907*; Science and Technology Research Development Plan of Yan’an City, No. 2009KS-12*; Science and Technology Research Plan of Yan’an University, No. YD2008-04*

PDF

275

摘要：

背景：血管内皮生长因子及其受体在男性生殖领域中占有重要地位，但其在生殖系统中表达的意义和调节机制仍不十分清楚。
目的：观察血管内皮生长因子及其受体类fms酪氨酸激酶在青春期大鼠附睾内精子上的表达定位情况。
方法：取10只青春期雄性SD大鼠双侧附睾，分离得到浓度为(30~40)×109 L-1的精子，涂片，免疫荧光法检测精子上血管内皮生长因子及其受体类fms酪氨酸激酶的表达定位情况。
结果与结论：免疫荧光结果显示，血管内皮生长因子及其受体类fms酪氨酸激酶阳性蛋白均定位于大鼠附睾精子头部的顶体、尾部的颈段、中段和主段，尾部末段和精子核未见阳性染色。提示，血管内皮生长因子及其受体类fms酪氨酸激酶可能参与了精子的成熟过程，与精子的运动能力、获能和顶体反应有关。 

关键词: 血管内皮生长因子, 受体, 类fms酪氨酸激酶, 精子, 免疫荧光

Abstract:

BACKGROUND: Vascular endothelial growth factor (VEGF) and its receptor fms-like tyrosine kinase-1 (Flt-1) have a very important position in the field of male reproduction. However, it is still unclear about their expression meaning and regulatory mechanism in the reproductive system.
OBJECTIVE: To study the expression and location of VEGF and Flt-1 in the epididymal sperm of adolescent male rats.
METHODS: The expression of VEGF and Flt-1 was detected in 10 adolescent SD rats. The concentration of the sperm was (30-40)×109/L. Immunofluorescent staining was used for VEGF and Flt-1 expression and location in the sperm.
RESULTS AND CONCLUSION: Immunofluorescent staining showed that VEGF and Flt-1 were both localized in the acrosome of sperm head, as well as in the neck, middle and principal segment of sperm tail. Specific expression patterns of VEGF and Flt-1 in the sperm of rats display that they may participate in spermiotelcosis, relevant to movement, capacitation and acrosome reaction of the sperm.

中图分类号: