中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5197-5200.doi: 10.3969/j.issn.1673-8225.2011.28.017

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

过氧化氢诱导体外损伤性白内障模型的构建及晶状体上皮细胞的凋亡

刘珍珍，李平华，陶树新，杨  杨   

  1. 重庆医科大学附属第一医院眼科，重庆市  400016
  • 收稿日期:2011-02-06 修回日期:2011-05-07 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 李平华，教授，重庆医科大学附属第一医院眼科，重庆市 400016 liuzhenzhen0828@163.com
  • 作者简介:刘珍珍★，女，1983年生，山东省威海市人，汉族，重庆医科大学在读硕士。主要从事白内障、青光眼的临床及基础研究。 liuzhenzhen0828@163.com

Establishment of hydrogen peroxide-induced cataract model in vitro and apoptosis of lens epithelial cells

Liu Zhen-zhen, Li Ping-hua, Tao Shu-xin, Yang Yang   

  1. Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Received:2011-02-06 Revised:2011-05-07 Online:2011-07-09 Published:2011-07-09
  • Contact: Li Ping-hua, Professor, Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China liuzhenzhen0828@163.com
  • About author:Liu Zhen-zhen★, Studying for master’s degree, Department of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China liuzhenzhen0828@163.com

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365

被引次数

8

摘要：

背景：氧化应激能够诱导晶状体上皮细胞发生凋亡。
目的：观察Fas蛋白与过氧化氢诱发白内障中晶状体上皮细胞凋亡的关系以及表没石子儿茶素没食子酸酯对Fas蛋白表达及晶状体上皮细胞凋亡的影响。
方法：将健康成年兔透明晶状体随机分为3组：空白对照组仅加入DMEM培养液，过氧化氢组加入DMEM+过氧化氢，表没石子儿茶素没食子酸酯组加入DMEM+过氧化氢+表没石子儿茶素没食子酸酯。
结果与结论：各组体外培养72 h后过氧化氢组晶状体上皮细胞凋亡率及Fas蛋白阳性表达率显著高于空白对照组(P < 0.05)。表没石子儿茶素没食子酸酯组晶状体上皮细胞凋亡率及Fas蛋白阳性表达率显著低于过氧化氢组(P < 0.05)。结果显示，过氧化氢可能是通过上调Fas蛋白的表达诱导晶状体上皮细胞凋亡，而抗氧化剂表没石子儿茶素没食子酸酯可下调Fas蛋白的表达而减轻晶状体上皮细胞的凋亡。

关键词: 晶状体上皮细胞, 过氧化氢, Fas蛋白, 表没石子儿茶素没食子酸酯, 凋亡, 白内障, 细胞模型, 组织构建

Abstract:

BACKGROUND: Oxidative stress can induce apoptosis in lens epithelial cells (LECs).
OBJECTIVE: To observe the connection of Fas with apoptosis of LECs in hydrogen peroxide (H2O2)-induced cataract and the effect of epigallocatechin-3-gallate (EGCG) on the expression of Fas and apoptosis of LECs.
METHODS: Lenses isolated from healthy adult rabbits were randomly divided into the blank control group (treated with DMEM), the H2O2 group (treated by DMEM + H2O2), and the EGCG group (treated by DMEM+H2O2 + EGCG).
RESULTS AND CONCLUSION: The apoptosis rate for LECs and the expression of Fas protein in H2O2 group were apparently higher than that in the blank control group (P < 0.05). Comparing with H2O2 group, apoptosis rate for LECs and the expression of Fas protein were significantly lower in EGCG group (P < 0.05). Results indicate that up-regulating the expression of Fas protein could be one of the mechanism through that H2O2 induced apoptosis of LECs. EGCG could down-regulate expression of Fas protein and reduce apoptosis of LECs.

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