中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5165-5168.doi: 10.3969/j.issn.1673-8225.2011.28.009

• 骨组织构建 bone tissue construction • 上一篇    下一篇

骨保护蛋白和骨保护蛋白配体在脊柱结核病灶骨中的表达

何玉泽1，王自立2，马  腾2 ，辛  兵1，郭开今1   

  1. 1徐州医学院附属医院骨科，江苏省徐州市  221002
    2宁夏医科大学附属医院骨科，宁夏回族自治区银川市  750004
  • 收稿日期:2011-04-27 修回日期:2011-06-01 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 何玉泽，硕士，主治医师。徐州医学院附属医院骨科，江苏省徐州市 221002 surgeonhyz@ sina.com
  • 作者简介:何玉泽★，男，1971年生，汉族，宁夏医科大学毕业，硕士，主治医师，主要从事骨科方面研究。 surgeonhyz@ sina.com

Expression of osteoprotegerin and osteoprotegerin ligand in the focus of spinal tuberculosis

He Yu-ze1, Wang Zi-li2, Ma Teng2, Xin Bing1, Guo Kai-jin1   

  1. 1Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou  221002, Jiangsu Province, China; 2Department of Orthopedics, Affiliated Hospital of Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
  • Received:2011-04-27 Revised:2011-06-01 Online:2011-07-09 Published:2011-07-09
  • Contact: He Yu-ze★, Master, Attending physician, Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China surgeonhyz@sina. com
  • About author:He Yu-ze★, Master, Attending physician, Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China surgeonhyz@sina. com

PDF

280

摘要：

背景：脊柱结核病灶骨组织硬化的病理过程较为复杂，其发生机制尚不明确。
目的：探讨脊柱结核病灶骨不同部位的骨保护蛋白和骨保护蛋白配体的表达。
方法：取脊柱结核患者脊柱结核术中切除的骨质，按照部位分成硬化骨组，亚正常骨组和正常骨组。应用PV-6000二步法免疫组化检测技术测定各组标本中骨保护蛋白和骨保护蛋白配体的平均吸光度及阳性面积比，并进行统计学分析。
结果与结论：脊柱结核硬化骨组骨保护蛋白平均吸光度，阳性面积比以及骨保护蛋白和骨保护蛋白配体表达的比值均显著高于亚正常骨组和正常骨组(P < 0.01或P < 0.05)。结果表明骨保护蛋白和骨保护蛋白配体的表达变化与脊柱结核病灶周围硬化骨的产生有关。

关键词: 脊柱结核, 硬化骨, 骨保护蛋白, 骨保护蛋白配体, 免疫组化

Abstract:

BACKGROUND: The pathological process of bone tissue hardening in spinal tuberculosis is complex, and the underlying mechanism remains poorly understood.
OBJECTIVE: To investigate the expression of osteoprotegerin (OPG) and OPG ligand (OPGL) in different sections of spinal tubercular infectious focus.
METHODS: The bones resected from patients with spinal tubereculosis were divided into three groups based on their regions: ossified bone, sub-normal bone and normal bone. The average absorbance and positive area percentage of OPG and OPGL in each group samples were detected by PV-6000 two-step immunohistochemical technique.
RESULTS AND CONCLUSION: The average absorbance and positive area percentage of OPG, as well as OPG and OPGL expression in the ossified bone group were significantly greater than in the normal bone group (P < 0.01 or P < 0.05). These findings suggest that changes in OPG and OPGL expression are related to the formation of ossified bone surrounding the focus of spinal tuberculosis.

中图分类号: