中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5169-5172.doi: 10.3969/j.issn.1673-8225.2011.28.010

• 骨组织构建 bone tissue construction • 上一篇    下一篇

小鼠成骨细胞Wnt信号通路相关因子表达与胰岛素样生长因子1的影响

郭  玲1，郑立舸1，王  敏2，郝  亮2   

  1. 1 泸州医学院附属口腔医院修复科，四川省泸州市  646000
    2 四川大学华西口腔医学院修复科，四川省成都市  610041
  • 收稿日期:2011-01-19 修回日期:2011-03-25 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 郑立舸，教授，泸州医学院附属口腔医院修复科，四川省泸州市 646000 Lzyxy-zlg@163. com
  • 作者简介:郭玲☆，女，1977年生，重庆市綦江县人，汉族，四川大学口腔医学院毕业，博士，讲师，主要从事口腔修复方面的研究。 glsmiling@ yahoo.cn
  • 基金资助:

    四川省卫生厅基金资助项目(20090201)，课题名称：IGF-1与Wnt/β-catenin信号通路在糖尿病颌骨骨质疏松的作用研究。

Effects of insulin-like growth factor 1 on expression of Wnt signaling pathway-related factors in mouse osteoblasts

Guo Ling1, Zheng Li-ge1, Wang Min2, Hao Liang2   

  1. 1Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou  646000, Sichuan Province, China
    2Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-01-19 Revised:2011-03-25 Online:2011-07-09 Published:2011-07-09
  • Contact: Zheng Li-ge, Professor, Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China Lzyxy-zlg@163.com
  • About author:Guo Ling☆, Doctor, Lecturer, Department of Prosthodontics, Stomatology Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China glsmiling@yahoo.cn
  • Supported by:

    Foundation of Ministry of Healthy of Sichuan Province, No. 20090201*

PDF

358

被引次数

3

摘要：

背景：胰岛素样生长因子1可促进细胞增殖、分化，但其具体作用机制尚不清楚。
目的：观察胰岛素样生长因子1对小鼠成骨细胞增殖﹑分化的影响，及Wnt信号通路相关因子mRNA的表达。
方法：向体外培养的小鼠成骨细胞中加入25 μg/L的胰岛素样生长因子1，分别于培养的第1，2，3，4，5天用CCK-8比色法检测细胞增殖率；于培养的第3，6，9天应用酶联免疫法检测细胞内碱性磷酸酶活性；细胞培养第3天提取总RNA，采用Real-time RT-PCR检测Wnt-3a，低密度脂蛋白受体相关蛋白5，β-catenin mRNA的表达。
结果与结论：25 μg/L胰岛素样生长因子1能够促进成骨细胞增殖，提高碱性磷酸酶活性，而且明显增加成骨细胞中Wnt-3a、低密度脂蛋白受体相关蛋白5、β-catenin mRNA的表达(P < 0.05)。说明胰岛素样生长因子1能够促进成骨细胞的增殖和分化，Wnt信号通路参与了该调控过程。

关键词: 胰岛素样生长因子1, 成骨细胞, 增殖, 分化, Wnt/&beta, -catenin

Abstract:

BACKGROUND: Insulin-like growth factor 1 (IGF-1) plays a crucial role in cell occurrence and development by regulating cell proliferation, differentiation and inhibition of apoptosis. But the underlying mechanism is unclear.
OBJECTIVE: To investigate the effects of IGF-1 on the proliferation and differentiation of the cultured mouse osteoblasts and the mRNA expression levels of some related factors of Wnt signaling pathway.
METHODS: The medium containing 25 μg/L IGF-1 was added to the mouse osteoblasts cultured in vitro. Cell proliferation was determined using CCK-8 method on days 1, 2, 3, 4, and 5 of culture. Alkaline phosphatase activity was determined with enzyme-linked immunosorbent assay on days 3, 6 and 9 days of culture. On day 3 of culture, total RNA was extracted, and the mRNA expression levels of Wnt-3a, low density lipoprotein receptor-related protein 5 and β-catenin were detected by real time polymerase chain reaction.
RESULTS AND CONCLUSION: 25 μg/L IGF-1 can promote the proliferation of mouse osteoblasts, increase the activity of alkaline phosphatase, and significantly increase the mRNA expression levels of Wnt-3a, low density lipoprotein receptor-related protein 5 and β-catenin (P < 0.05). These findings suggest that IGF-1 can promote the proliferation and differentiation of osteoblasts and Wnt signaling pathway is involved in this process.

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