中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5108-5111.doi: 10.3969/j.issn.1673-8225.2011.27.038

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

骨髓间充质干细胞的分离培养与鉴定

胡  彬，刘晓华，赵  林   

  1. 青岛市中心血站输血医学研究所，山东省青岛市 266071
  • 收稿日期:2011-01-27 修回日期:2011-05-10 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 赵林，主任医师，青岛市中心血站输血医学研究所，山东省青岛市 266071 zhaolinqd@163.com
  • 作者简介:胡彬★，女，1973年生，四川省大足县人，汉族，2005年青岛大学医学院毕业，硕士，主治医师，主要从事输血医学的研究。 huh_138@hotmail.com
  • 基金资助:

    2009年青岛市公共领域科技支撑计划项目(09-1-1-25-nsh)。

Isolation, cultivation and identification of bone marrow mesenchymal stem cells

Hu Bin, Liu Xiao-hua, Zhao Lin   

  1. Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao  266071, Shandong Province, China
  • Received:2011-01-27 Revised:2011-05-10 Online:2011-07-02 Published:2011-07-02
  • Contact: Zhao Lin, Chief physician, Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao 266071, Shandong Province, China zhaolinqd@163.com
  • About author:Hu Bin★, Master, Attending physician, Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao 266071, Shandong Province, China huh_138@hotmail.com
  • Supported by:

    Qingdao Science and Technology Support Program in the Public Domain in 2009, No. 09-1-1-25-nsh*

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摘要：

背景：近年来骨髓间充质干细胞在组织工程、细胞移植、基因治疗等领域备受重视，得到广泛研究。
目的：对骨髓间充质干细胞的生物学特性、体外分离、培养及鉴定方法及其临床学的应用进行综述。
方法：应用计算机检索1995-01/2010-01 CNKI数据库相关文章，检索词为“骨髓间充质干细胞，分离，培养，鉴定”，并限定文章语言种类为中文。同时计算机检索1995-01/2010-01 PubMed数据库相关文章，检索词为“bone marrow mesenchymal stem cells，isolation，cultivation，identification”，并限定文章语言种类为“English”。共检索到文献68篇，最终纳入符合标准的文献30篇。
结果与结论：骨髓间充质干细胞是存在于骨髓中的一类非造血干细胞，易于获得和分离培养，且具有多向分化及高度增殖的能力。目前研究发现它具有分化为成骨细胞、软骨细胞、脂肪细胞、心肌细胞、神经细胞以及肝细胞等多种类型细胞的潜能，已经成为重要的组织工程种子细胞。

关键词: 骨髓间充质干细胞, 分离, 培养, 鉴定, 综述文献

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMMSCs) are currently a hot subject of research in many fields such as tissue engineering, cells transplantation and gene therapy.
OBJECTIVE: To review the biological property, isolation, cultivation and clinical application of BMMSCs.
METHODS: We had retrieved 30 relevant articles from CNKI and PubMed databases with the key words of “bone marrow mesenchymal stem cells, isolation, cultivation, identification” in Chinese and English, respectively, between January 1995 to January 2010 respectively.
RESULTS AND CONCLUSION: BMMSCs are nonhematopoietic stem cells and easy to be harvested and cultured which show varying differentiation and high proliferation potentials. BMMSCs can differentiate into osteoblasts, chondrocytes, lipocytes, myocardial cells, nerve cells and hepatocytes, and have become an important seed cells for tissue engineering.

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