中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5080-8084.doi: 10.3969/j.issn.1673-8225.2011.27.031

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

注射用七叶皂苷钠联合脐带间充质干细胞移植改善脑梗死大鼠神经功能

陈雪扉1，莫万斌2    

  1. 桂林市第二人民医院，1神经内科，2神经外科，广西壮族自治区桂林市  541001
  • 收稿日期:2011-05-16 修回日期:2011-06-20 出版日期:2011-07-02 发布日期:2011-07-02
  • 作者简介:陈雪扉★，女，1969年生，广西壮族自治区桂林市人，汉族，广西医科大在读硕士，主治医师，主要从事神经修复研究。 sufeiyasufeiya@163.com

Aescine sodium combined with umbilical cord mesenchymal stem cells transplantation for treatment of neurological function in rats with cerebral infarction

Chen Xue-fei1, Mo Wan-bin2   

  1. 1Department of Neurology, 2Department of Neurosurgery, Guilin Second People's Hospital, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-16 Revised:2011-06-20 Online:2011-07-02 Published:2011-07-02
  • About author:Chen Xue-fei★, Studying for master’s degree, Attending physician, Department of Neurology, Guilin Second People's Hospital, Guilin 541001, Guangxi Zhuang Autonomous Region, China sufeiyasufeiya@163.com

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394

被引次数

11

摘要：

背景：单纯的脐带间充质干细胞移植修复受损脑组织的作用并不十分理想。
目的：观察脐带间充质干细胞移植联合注射用七叶皂苷钠治疗大鼠脑梗死的效果。
方法：应用线栓法建立大鼠大脑中动脉阻塞模型，随机分为对照组、细胞移植组、七叶皂苷钠+ 细胞移植组，分别尾静脉注射细胞培养液、1×1010 L-1脐带间充质干细胞悬液、尾静脉1×1010 L-1脐带间充质干细胞悬液同时经腹腔注射七叶皂苷钠 5 mg/(kg•d)，连续5 d。
结果与结论：移植后1周，七叶皂苷钠+细胞移植组大鼠神经功能障碍评分低于细胞移植组及对照组(P < 0.05)；七叶皂苷钠+细胞移植组大鼠脑梗死周围组织AQP9 及AQP4 mRNA的表达低于细胞移植组，却高于对照组(P < 0.05)；七叶皂苷钠+细胞移植组CM-Dil阳性细胞和神经元数量多于细胞移植组及对照组(P < 0.05)。提示脐带间充质干细胞移植联合注射用七叶皂苷钠治疗大鼠脑梗死可明显改善大鼠的神经功能。

关键词: 七叶皂苷钠, 脐带间充质干细胞, 移植, 脑梗死, AQP4, AQP9

Abstract:

BACKGROUND: The effect of single umbilical cord derived mesenchymal stem cells (UC-MSCs) transplantation repair damaged brain tissue is not very satisfactory.
OBJECTIVE: To investigate the effect of UC-MSCs transplantation combined with aescine sodium on the treatment of cerebral infarction in rats.
METHODS: The models of cerebral infarction were made by middle cerebral artery occlusion (MCAO). Rats were divided randomly into control group, UC-MSCs group and Aescine sodium + UC-MSCs group. The concentration of 1×1010/L UC-MSCs suspension or the concentration of 1 mL cell culture fluid was injected into MCAO models through caudal vein, at the same time,
5 mg/(kg•d) aescine sodium aqueous solution or normal saline was injected into MCAO models through abdominal cavity, for 5 days.
RESULTS AND CONCLUSION: The neurological dysfunction scores in aescine sodium + UC-MSCs group were significantly lower than those in UC-MSCs group and control group at 1 week after transplantation (P < 0.05). RT-PCR approved that the expression of AQP4 and AQP9 mRNA was highest in control group, a few in UC-MSCs group and lowest in aescine sodium + UC-MSCs group (P < 0.05). The number of CM-Dil-positive cells and neurons in aescine sodium + UC-MSCs group was higher than that in UC-MSCs group and control group (P < 0.05). The results suggested that UC-MSCs transplantation combined with aescine sodium can significantly improve neurological function of rats with cerebral infarction.

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