中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5058-5062.doi: 10.3969/j.issn.1673-8225.2011.27.026

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

基质细胞衍生因子1趋化神经干细胞迁移的体外效应

牟临杰，丁  鹏，王崇谦，王伟民，李宣鹏，王进昆   

  1. 昆明医学院第一附属医院神经外科，云南省昆明市  650032
  • 收稿日期:2011-01-16 修回日期:2011-03-24 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 王进昆，主任医师，硕士生导师，昆明医学院第一附属医院神经外科，云南省昆明市650032
  • 作者简介:牟临杰★，男，1984年生，甘肃省临夏市人，汉族，昆明医学院第一附属医院神经外科在读硕士。主要从事神经干细胞基础研究及脑胶质瘤的体外诱导凋亡。 mulinjie84@gmail.com 并列第一作者：丁鹏☆，男，1976年生，山东省淄博市人，汉族，2005年昆明医学院毕业，博士，副教授，主要从事干细胞研究及神经外科方面的研究。
  • 基金资助:

    2008年云南应用基础研究面上项目(2008ZC132M)。

In vitro effect of stromal cell derived factor -1 on the migration of neural stem cells

Mu Lin-jie, Ding Peng, Wang Chong-qian, Wang Wei-min, Li Xuan-peng, Wang Jin-kun   

  1. Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2011-01-16 Revised:2011-03-24 Online:2011-07-02 Published:2011-07-02
  • Contact: Wang Jin-kun, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Mu Lin-jie★, Studying for master’s degree, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China mulinjie84@gmail.com Ding Peng☆, Doctor, Associate professor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Mu Lin-jie and Ding Peng contributed equally to this paper.
  • Supported by:

    Applied Basic Research of Yunnan Province in 2008, No. 2008ZC132M*

PDF

511

被引次数

13

摘要：

背景：神经干细胞的迁移在神经系统的发育和损伤修复中起着至关重要的作用，近来研究表明趋化因子参与神经干细胞的迁移，但关于其迁移机制目前尚不清楚。
目的：观察体外条件下基质细胞衍生因子1对胎鼠海马神经干细胞的趋化迁移作用。
方法：通过无血清法体外分离、培养及鉴定胎鼠海马神经干细胞；细胞免疫荧光及RT-PCR检测其CXCR4是否表达；观察不同浓度基质细胞衍生因子1对神经干细胞的趋化迁移作用，中和CXCR4受体以验证基质细胞衍生因子1趋化迁移作用的特异性。
结果与结论：胎鼠海马来源神经干细胞表达趋化因子受体CXCR4，呈阳性。RT-PCR琼脂糖凝胶电泳后出现643 bp特异性扩增条带。体外条件下基质细胞衍生因子1趋化迁移随浓度而增强，500 μg/L为最佳趋化浓度。加入抗CXCR4多克隆抗体中和后，神经干细胞迁移较基质细胞衍生因子1组明显减少，与对照组比较，差异无显著性意义(P > 0.05)，提示抗CXCR4多克隆抗体可阻断趋化迁移作用。

关键词: 基质细胞衍生因子1, 神经干细胞, 趋化因子, 细胞迁移, CXCR4

Abstract:

BACKGROUND: Neural stem cell migration plays important roles in the development and repair of the central nervous system. Although recent studies have shown that the chemokines mediate neural stem cell migration, but the mechanism is unclear.
OBJECTIVE: To explore the effects of stromal cell-derived factor-1 (SDF-1) on migration of fetal rat hippocampus neural stem cells in vitro.
METHODS: The fetal rat hippocampal neural stem cells were isolated, cultured and identified in serum-free medium. The expression of CXCR4 in neural stem cells was detected using immunofluorescence and RT-PCR. The under-agarose cell migration assay was used to observe the effects of SDF-1(50-500 ng/ml) on neural stem cell migration, and blocking CXCR4, the receptor of SDF-1, to identify the specificity of migration.
RESULTS AND CONCLUSION: Immunofluorescence results showed that neural stem cells were CXCR4 positive, and RT-PCR findings showed 643 bp specific band in agarose gel electrophoresis. The Under-Agarose cell migration assay showed that: SDF-1 (50-500 ng/ml) could accelerate the migration of neural stem cells, the migration increased with the concentration, and 500 μg/L was the best. By adding anti-CXCR4 polyclonal antibodies, the migration of neural stem cells compared with SDF-1 was significantly reduced, no significant difference with the control group (P > 0.05), pointing out anti-CXCR4 polyclonal antibodies can block the effect of the migration.

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