中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 5063-5067.doi: 10.3969/j.issn.1673-8225.2011.27.027

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

软骨损伤后基质细胞衍生因子1的表达

陆定贵1，韦庆军1，李桥川2，韦积华1，肖仕辉1，李伟岸1   

  1. 广西医科大学第一附属医院，1创伤骨科手外科，2干细胞移植中心，广西壮族自治区南宁市530021
  • 收稿日期:2011-03-02 修回日期:2011-06-09 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 韦庆军，博士，副教授，广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021 weiqingjungxnn@163.com
  • 作者简介:陆定贵★，男，1983年生，广西壮族自治区崇左市人，壮族，广西医科大学在读硕士，主要从事软骨组织工程研究。 ludinggui@163.com
  • 基金资助:

    课题受广西留学回国人员科学基金(305696)资助。

Expression of stromal cell-derived factor-1 after articular cartilage injury

Lu Ding-gui1, Wei Qing-jun1, Li Qiao-chuan2, Wei Ji-hua1, Xiao Shi-hui1, Li Wei-an1   

  1. 1Department of Trauma, 2Stem Cell Transplantation Centre, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-03-02 Revised:2011-06-09 Online:2011-07-02 Published:2011-07-02
  • Contact: Wei Qing-jun, Doctor, Associate professor, Department of Trauma, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China weiqingjungxnn@163.com
  • About author:Lu Ding-gui★, Studying for master’s degree, Department of Trauma, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China ludinggui@163.com
  • Supported by:

    the Scientific Research Foundation for the Returned Overseas Chinese Scholars in Guangxi Zhuang Autonomous Region, No. 305696*

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摘要：

背景：研究表明基质细胞衍生因子1对骨髓间充质干细胞的迁移、聚集有影响。
目的：观察软骨损伤后不同时间损伤修复区组织基质细胞衍生因子1的表达以及与骨髓间充质干细胞迁移的关系。
方法：建立兔软骨损伤模型，分别于建模后2，5，7，10，14，28 d取损伤灶及边缘区组织，检测基质细胞衍生因子1表达和体外细胞迁移实验，观察基质细胞衍生因子1对骨髓间充质干细胞、软骨细胞的迁移影响。
结果与结论：基质细胞衍生因子1的表达呈现出时间变化的趋势，软骨损伤后第7天达到高峰(P < 0.05)。体内移植的骨髓间充质干细胞主要聚集在软骨损伤灶周围，但在封闭CXC趋化因子受体4后，此聚集现象逐渐减弱(P < 0.05)。结果证实，局部组织中基质细胞衍生因子1的表达在软骨损伤早期明显升高，对骨髓间充质干细胞向软骨损伤修复区迁移有重要作用。

关键词: 基质细胞衍生因子1, 骨髓间充质干细胞, CXC趋化因子受体4, 软骨损伤, 组织工程

Abstract:

BACKGROUND: Studies have demonstrated that stromal cell-derived factor-1 (SDF-1) can influence the migration and aggregation of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To observe the expression of SDF-1 at lesion regions at different time after cartilage injury and to investigate the correlation between SDF-1 expression and BMSCs migration.
METHODS: Rabbits models of articular cartilage injury were established. Focal osteochondral lesions were collected 2, 5, 7, 10, 14 and 28 days after injury. The influence of SDF-1 expression on the migration of BMSCs and chondrocytes was observed.
RESULTS AND CONCLUSION: SDF-1 expression was significantly increased and peaked at the seventh day (P < 0.05). BMSCs migrated toward articular cartilage injury tissue, while they were significantly inhibited by the inhibitor of CXCL4. SDF-1 expression increases in the early phase after articular cartilage injury, and plays an important role in chemoattracting MSCs to injured lesions.

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