中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (27): 4955-4958.doi: 10.3969/j.issn.1673-8225.2011.27.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

密度梯度离心法体外培养骨髓间充质干细胞的分化能力

穆晓红1，赵子义2，徐  林2，王硕仁3，朱陵群3，刘铜华1   

  1. 1北京中医药大学，北京市  100029；北京中医药大学，2东直门医院骨科，3中心实验室，北京市  100700
  • 收稿日期:2011-03-19 修回日期:2011-05-31 出版日期:2011-07-02 发布日期:2011-07-02
  • 通讯作者: 刘铜华，博士后，博士生导师，主任医师，教授，北京中医药大学，北京市 100029 thliu@tom.com
  • 作者简介:穆晓红☆，女，1972年生，河南省郑州市人，汉族，北京中医药大学在站博士后，副主任医师，主要从事干细胞生物学及神经系统损伤方面的研究。 Mxh_2004@163.com
  • 基金资助:

    国家自然科学基资助项目(30600847)。

Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro

Mu Xiao-hong1, Zhao Zi-yi2, Xu Lin2, Wang Shuo-ren3, Zhu Ling-qun3, Liu Tong-hua1   

  1. 1Beijing University of Chinese Medicine, Beijing  100029, China; 2Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing  100700, China; 3Central Laboratory,  Beijing University of Chinese Medicine, Beijing  100700, China
  • Received:2011-03-19 Revised:2011-05-31 Online:2011-07-02 Published:2011-07-02
  • Contact: Liu Tong-hua, Doctor, Doctoral supervisor, Chief physician, Professor, Beijing University of Chinese Medicine, Beijing 100029, China thliu@tom.com
  • About author:Mu Xiao-hong☆, Doctor, Associate chief physician, Beijing University of Chinese Medicine, Beijing 100029, China mxh_2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600847*

PDF

466

被引次数

26

摘要：

背景：骨髓间充质干细胞具有多向分化能力，是目前极具潜力的种子细胞及基因治疗的靶细胞。
目的：体外培养兔骨髓来源间充质干细胞，了解其生物学特性。
方法：采用密度梯度离心法培养兔骨髓间充质干细胞，对细胞的形态及生长特性进行观察，应用流式细胞仪检测细胞表面抗原CD29、CD34、CD44、CD45、CD166、HLA-DR表达，并进行相关生物学特性鉴定。
结果与结论：密度梯度离心法能分离培养出纯度较高的骨髓间充质干细胞，流式细胞仪检测骨髓间充质干细胞表达CD29、CD44、CD166，不表达CD34、CD45、HLA-DR。在适当的条件下能够诱导分化成为成骨细胞、软骨细胞等。

关键词: 骨髓间充质干细胞, 分离, 培养, 分化, 生物学特性

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are capable of multiple directional differentiation, and are potential seed cells and target cells for gene therapy.
OBJECTIVE: To observe in vitro cultured rabbit BMSCs, to understand its biological properties.
METHODS: Density gradient centrifugation cultivation was utilized to culture rabbit BMSCs. The morphology and characteristics of cultured cells were observed. The surface markers CD29, CD34, CD44, CD45, CD166 and HLA-DR of cells were identified by flow cytometry, and its biological properties were identified.
RESULTS AND CONCLUSION: High purity of rabbit BMSCs could be achieved by the density gradient centrifugation. The expressions of cell phenotypes were shown as follows respectively: CD29+, CD34-, CD44+, CD45-, CD166+, HLA-DR-. Under appropriate conditions, BMSCs were induced to differentiate into osteoblasts and cartilage cells.

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