中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4344-4350.doi: 10.3969/j.issn.1673-8225.2011.23.038

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

诱导多功能干细胞的研究进展

王  格1，陈  哲1，武栋成2   

  1. 1武汉大学，湖北省武汉市430071
    2武汉大学生物化学系，武汉红桥脑科医院，湖北省武汉市 430071
  • 收稿日期:2010-12-02 修回日期:2011-02-20 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 武栋成，博士生导师，武汉大学生物化学系，武汉红桥脑科医院，湖北省武汉市 430071 bcdcwu@hotmail.com
  • 作者简介:王格，女，1989年生，湖南省衡阳县人，汉族，武汉大学本硕博连读，主要从事干细胞研究。 gege3192@126.com
  • 基金资助:

    国家自然科学基金项目(面上项目，30670813)。

Progress regarding induction of pluripotent stem cells

Wang Ge1, Chen Zhe1, Wu Dong-cheng2   

  1. 1Wuhan University, 2Department of Biochemistry, Wuhan University, Wuhan Hongqiao Brain Hospital, Wuhan  430071, Hubei Province, China
  • Received:2010-12-02 Revised:2011-02-20 Online:2011-06-04 Published:2011-06-04
  • Contact: Wu Dong-cheng, Doctoral supervisor, Department of Biochemistry, Wuhan Hongqiao Brain Hospital, Wuhan University, Wuhan 430071, Hubei Province, China bcdcwu@hotmail.com
  • About author:Wang Ge, Wuhan University, Wuhan 430071, Hubei Province, China gege3192@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30670813*

PDF

1308

被引次数

13

摘要：

背景：由于胚胎干细胞移植存在致瘤性和伦理学争议，有关胚胎干细胞的研究及临床应用存在较大的限制。2006年Yamanaka实验室利用Oct3/4、Sox2、Klf4、c-Myc 4 种因子将鼠成纤维细胞重编程为诱导多功能干细胞，标志着一种新型类胚胎干细胞的问世。
目的：了解诱导多功能干细胞的研究进展和应用前景。
方法：由第一作者检索2006/2010 PubMed 数据库(http://www.ncbi.nlm.nih.gov/PubMed)及万方数据库(http://g.wanfangdata.com.cn/)有关诱导多功能干细胞的产生、细胞特征、产生技术的研究进展及应用前景等方面的文章，英文检索词为“induced pluripotent stem cells，defined factors，reprogramming，vectors，disease”，排除重复性研究，共保留其中的69篇进行归纳总结。
结果与结论：诱导多功能干细胞研究在诱导因子种类，因子导入方式，重编程效率及应用研究等诸多方面取得进展。然而体细胞重编程为诱导多功能干细胞仍存在一定的风险，重编程效率还非常低。一旦解决诱导多功能干细胞的安全性和重编程效率问题，诱导多功能干细胞就可被广泛应用于疾病模型，药物测试，细胞移植及患者和疾病特异性多功能干细胞的建立等诸多方面。

关键词: 诱导多功能干细胞, 重编程, 因子, 载体, 效率

Abstract:

BACKGROUND: There are large research and clinical application limitations on embryonic stem cells (ESCs) considering its potential oncogenicity and ethical disputes. In 2006, Yamanaka found that four factors (Oct3/4, Sox2, Klf4, c-Myc) could induce mouse embryonic fibroblasts into an embryonic stem cell-like state, which is called induced pluripotent stem cells (iPSCs). This shows the appearance of a new kind of embryonic stem cell-like cells.
OBJECTIVE: To understand progress and application of iPSCs in the field of stem cells.
METHODS: The first author searched PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang database (http://g.wanfangdata.com.cn/) for literatures concerning iPSCs generation, characteristics, and research progress from 2006 to 2010. Key words were “induced pluripotent stem cells, defined factors, reprogramming, vectors, disease”. Duplicate articles were excluded. Finally, 69 articles were selected.
RESULTS AND CONCLUSION: Great progress has been made on iPSCs, includes the kind of factors, vectors, reprogramming efficiency and application research. However, there are some obstacles on somatic cells reprogramming such as potential damage of exogenous factors and vectors to cells low reprogramming efficiency. Once these problems solved, iPSCs can be widely used for disease modeling, drug test, cell transplantation and production of patient-specific iPSCs.

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