中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4316-4320.doi: 10.3969/j.issn.1673-8225.2011.23.031

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

静脉移植CXCR-4基因转染骨髓间充质干细胞在脊髓损伤鼠体内的迁移与分化

宋晨阳1，徐  皓2，陈建梅2，潘廷明3，姚晓东2   

  1. 1福建医科大学附属协和医院，福建省福州市350001
    2解放军南京军区福州总医院，福建省福州市 350025
    3福建中医药大学附属第二人民医院，福建省福州市350003
  • 收稿日期:2011-01-09 修回日期:2011-02-12 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 徐 皓，解放军南京军区福州总医院骨科主任，解放军南京军区骨科研究所所长，主任医师，教授，解放军南京军区福州总医院，建省福州市 350025 xiuhao@medmail.com.cn
  • 作者简介:宋晨阳★，男，1980年生，福建省福州市人，汉族， 2009年福建医科大学毕业，硕士，医师，主要从事脊柱外科研究。 charliesong86@163.com
  • 基金资助:

    南京军区“十一•五”医药卫生科研重点基金资助项目(07Z037)，课题名称：调控SDF-1/CXCR-4促进神经样BMSCs定向迁移修复脊髓损伤的实验研究。

Immigration and differentiation of bone marrow mesenchymal stem cells transfected by CXCR-4 gene after intravenous transplantation in mice with spinal cord injury

Song Chen-yang1, Xu Hao2, Chen Jian-mei2, Pan Ting-ming3, Yao Xiao-dong2   

  1. 1Union Hospital of Fujian Medical University, Fuzhou  350001, Fujian Province, China
    2Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou  350025, Fujian Province, China
    3Second Affiliated People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou  350003, Fujian Province, China
  • Received:2011-01-09 Revised:2011-02-12 Online:2011-06-04 Published:2011-06-04
  • Contact: Xu Hao, Chief physician, Professor, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China xiuhao@medmail.com.cn
  • About author:Song Chen-yang★, Master, Physician, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China charliesong86@163.com
  • Supported by:

    the Key “Eleventh-Five Year” Medical and Health Research Program of Nanjing Military Area Command of Chinese, PLA, No. 07Z037*

PDF

287

被引次数

4

摘要：

背景：研究发现，基质细胞衍生因子1/CXCR-4轴具有介导骨髓间充质干细胞定向迁移的作用。
目的：观察静脉移植CXCR-4基因转染间充质干细胞治疗脊髓损伤的可行性。
方法：利用脊髓横切法构建C57BL/6小鼠T10脊髓损伤模型，造模后7 d抽签随机分成3组：实验组经尾静脉注射CXCR-4基因转染同种异体骨髓间充质干细胞悬液，对照组与空白对照组分别注射同种异体骨髓间充质干细胞悬液及无细胞培养基。移植后第7，14，21，28天利用激光共聚焦显微镜、免疫组织化学双标染色法检测脊髓损伤部位绿色荧光蛋白阳性细胞迁移存活及分化情况，并采用BBB评分评估小鼠神经运动功能恢复情况。
结果与结论：实验组移植后各时相点损伤灶内绿色荧光蛋白阳性细胞集聚明显，数量不断增多，迁移率明显高于对照组与空白对照组，来源于骨髓间充质干细胞的神经细胞占移植细胞的比例亦高于对照组与空白对照组，小鼠神经运动功能恢复明显。说明静脉移植CXCR-4基因转染的骨髓间充质干细胞表现出对脊髓损伤灶更强的定向迁移能力，且能在损伤灶内存活、分化，发挥修复损伤脊髓作用。

关键词: 定向迁移, 间充质干细胞, CXCR-4基因, 脊髓损伤, 转染, 移植

Abstract:

BACKGROUND: Previous studies demonstrated that stromal cell derived factor-1/CXCR-4 axis system plays a role in mediating bone marrow mesenchymal stem cells’ (BMSCs) orient immigration.
OBJECTIVE: To investigate the feasibility of BMSCs transfected by CXCR-4 gene after intravenous transplantation for treatment of spinal cord injury (SCI).
METHODS: C57BL/6 mice T10 SCI model was constructed by spinal cord transection method, and at 7 days after modeling, which were randomly divided into 3 groups: experimental group, control group and blank group. Mice were intravenous injected by the suspensions of allogeneic BMSCs transfected by CXCR-4 gene in experimental group. Mice were respectively intravenous injected by the suspensions of non-transfected allogeneic BMSCs and non-cell culture medium in control and blank groups. At 7, 14, 21, 28 days after transplantation, the survival and differentiation of green fluorescence protein positive cells to all cells in different site after transplantation were detected by laser scanning confocal microscope and Immunohistochemical double staining method. BBB score was used to evaluate the recovery condition of neuromotor function of mice.
RESULTS AND CONCLUSION: After transplantation, green fluorescence protein positive cells significantly gathered in the injured part of spinal cord in experimental group at each time point, and the number gradually increased, and the transport factor was obviously higher than in control and blank groups. The proportion of neural cells from BMSCs in transplanted cells in experimental group was also higher than that in control and blank groups. The neuromotor function of mice recovered obviously. BMSCs transfected by CXCR-4 gene after intravenous transplantation showed stronger orient immigration ability to SCI focus, which can survive and differentiate in the SCI focus, and play a role in repairing SCI.

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