中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4185-4188.doi: 10.3969/j.issn.1673-8225.2011.23.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

大鼠骨髓间充质干细胞体外向成骨细胞分化培养的生物学特性

李雪峰1，刘  亮2，王  东2   

  1. 1北京市丰台区长辛店医院骨科，北京市  100043
    2山西医科大学第二附属医院骨科，山西省太原市030000
  • 收稿日期:2010-12-03 修回日期:2011-02-05 出版日期:2011-06-04 发布日期:2011-06-04
  • 作者简介:李雪峰★，男， 1975年生，山西省运城市人，汉族， 2007年山西医科大学第二附属医院毕业，硕士，主治医师，主要从事骨外科学研究。 lxf285639@163.com

Biological characteristics of in vitro differentiation of bone marrow stromal cells into osteoblasts in rats

Li Xue-feng1, Liu Liang2, Wang Dong2   

  1. 1Department of Orthopaedics, Changxindian Hospital, Beijing Fengtai District, Beijing  100043, China
    2Department of Orthopaedics, Second Affiliated Hospital of Shanxi Medical University, Taiyuan  030000, Shanxi Province, China
  • Received:2010-12-03 Revised:2011-02-05 Online:2011-06-04 Published:2011-06-04
  • About author:Li Xue-feng★, Master, Attending physician, Department of Orthopaedics, Changxindian Hospital, Beijing Fengtai District, Beijing 100043, China lxf285639@163.com

PDF

357

被引次数

15

摘要：

背景：骨髓间充质干细胞因取材方便、对机体损伤小，是目前最为合适的骨组织工程种子细胞，但其体外培养的具体方法及生物特性仍无定论。
目的：建立一种简单有效的骨髓间充质干细胞的体外培养及成骨诱导方法，并探讨其体外培养的生物学特点。
方法：采用贴壁法分离提纯Wistar大鼠骨髓间充质干细胞，传至第3代细胞后，分别在普通培养基(对照组)和成骨诱导培养基(实验组)中培养10 d，绘制细胞生长曲线。于培养第4，7，10，13，16天检测两组细胞碱性磷酸酶活性，并对细胞爬片行Von kossa染色。
结果与结论：采用贴壁法获得了均一性较好的骨髓间充质干细胞，对照组细胞生长速度明显快于实验组细胞。对照组细胞碱性磷酸酶活性明显低于实验组(P < 0.05)。实验组细胞Von kossa染色为阳性，对照组为阴性。说明贴壁筛选法是一种简单有效的骨髓间充质干细胞的分离纯化方法，骨髓间充质干细胞在体外可诱导分化为成骨细胞。

关键词: 体外培养, 骨髓间充质干细胞, 生物学特性, 诱导分化, 干细胞培养

Abstract:

BACKGROUND: Because of convenience and small injury to the body, bone marrow stromal cells (BMSCs) are the most appropriate seed cells in bone tissue engineering. But the methods in vitro cultivation and the biological characteristic are not clear. 
OBJECTIVE: To establish a simple and effective BMSCs cultured in vitro and osteoblasts induction method, and to explore the biological characteristics in vitro.
METHODS: The BMSCs of Wistar rats were seperated and purified by using of adherent method. The passage cells of the third generation were cultured in the common medium (control group) and osteogenic induction medium (experimental group) for 10 days, the growth cure of each group was drew. The alkaline phosphatase activities (APA) of two groups were detected at 4, 7, 10, 13, 16, days’ culture and cover glass was treated with Von Kossa staining.
RESULTS AND CONCLUSION: The better homogenicity of BMSCs were obtained by adherent method. The growth velocity of the cells cultured in the control group was obviously faster than that in the experimental group. The APA in control group was significantly lower than that in experimental group (P < 0.05). The Von Kossa’ staining in the experimental group was positive and negative in the control group. Adherent screening method is a simple and effective method to separate and purify BMSCs. BMSCs can be differentiated into osteoblasts in vitro.

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