中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4189-4193.doi: 10.3969/j.issn.1673-8225.2011.23.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞在IgA肾病大鼠肾脏中的定位与分布

彭  伟1，刘郑荣2   

  1. 1武警广东省总队医院肾内科，广东省广州市510507
    2南方医科大学附属南方医院肾内科，广东省广州市510515
  • 收稿日期:2010-12-22 修回日期:2011-02-16 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 刘郑荣，硕士，副主任医师，南方医科大学附属南方医院肾内科，广东省广州市 510515 phhw117@126.com
  • 作者简介:彭伟★，女，1977年生，湖南省长沙市人，汉族，2000年南方医科大学毕业，硕士，主治医师，主要从事肾脏病尤其是IgA肾病方面的研究。
  • 基金资助:

    广东省自然科学基金（7005161）资助项目。

Location and distribution of bone marrow mesenchymal stem cells in the kidney of IgA nephropathy rats

Peng Wei1, Liu Zheng-rong2   

  1. 1Department of Nephrology, Wu Jing Zong Dui Hospital of Guangdong Province, Guangzhou  510507, Guangdong Province, China
    2Department of Nephrology, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2010-12-22 Revised:2011-02-16 Online:2011-06-04 Published:2011-06-04
  • Contact: Liu Zheng-rong, Master, Associate chief physician, Department of Nephrology, Nanfang Hospital Affiliated to Southern Medical University, Guangzhou 510515, Guangdong Province, China phhw117@126.com
  • About author:Peng Wei★, Master, Attending physician, Department of Nephrology, Wu Jing Zong Dui Hospital of Guangdong Province, Guangzhou 510507, Guangdong Province, China
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 7005161*

PDF

627

被引次数

20

摘要：

背景：由于骨髓间充质干细胞缺乏特异性表面抗原，常根据其贴壁生长、成骨成脂分化等生物学特点来鉴定。
目的：观察骨髓间充质干细胞在IgA肾病大鼠肾脏中的定位与分布情况。
方法：以牛血清白蛋白+葡萄球菌肠毒素B+皮下注射四氯化碳的改良法建立SD大鼠IgA肾病模型。造模成功分别经尾静脉移植大鼠骨髓间充质干细胞或生理盐水，并设立不造模的正常对照组。移植后1，4周观察各组大鼠的尿蛋白、肾功能、肾脏病理变化、IgA荧光沉积变化，并通过免疫组织化学观察BrdU标记的骨髓间充质干细胞在肾组织中的分布情况。
结果与结论：移植后1周，骨髓间充质干细胞组尿蛋白、血肌酐明显低于生理盐水组；移植后4周，骨髓间充质干细胞组肾脏病理变化、IgA荧光沉积与生理盐水组比较差异有显著性意义。提示骨髓间充质干细胞输注可促进大鼠IgA肾病的修复，并可定位于肾小球系膜区及少数肾小管间质区，但随时间延长，BrdU标记的髓间充质干细胞在肾组织中分布却逐渐减少。

关键词: 间质干细胞, 骨髓细胞, IgA肾病, 尿嘧啶核苷酸类, 细胞因子

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) without specific surface antigens are often identified according to adhesive growth, osteogenic differentiation, adipogenic differentiation and other biological characteristics.
OBJECTIVE: To observe the location and distribution of BMSCs in IgA nephropathy rats. 
METHODS: Sprague-Dawley rats were randomly divided into three groups: BMSCs group, saline group and control group. IgA nephropathy model was established by the improving method with bovine serum albumin+staphylococcal enterotoxin B+subcutaneous injection of carbon tetrachloride in the former two groups. At the 1st and 4th weeks after BMSCs injection, the changes of urine protein, renal function, histopathology and IgA immunofluorescence were observed. BrdU-labeled BMSCs were detected by immunohistochemistry to observe the disposition in the kidney.
RESULTS AND CONCLUSION: At the end of the first week of BMSCs transplantation, levels of urine protein and serum creatinine in the BMSCs group were lower than those in the saline group. At the end of the 4th week, the histopathology and IgA immunofluorescence of BMSCs group had the statistical significance compared with those of the saline group. It was indicated that BMSCs injection contributed to renal repair in rat IgA nephropathy. Some of them located in the mesangium and a few were in tubule and interstitial area. But as time passed, the disposition of BrdU-labeled MSCs in kidney was decreased gradually.

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