中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3737-3740.doi: 10.3969/j.issn.1673-8225.2011.20.030

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

正畸牙移动过程中牙周组织白细胞介素8的表达

潘  旭，米丛波，刘  红，钱雅婧，聂  晶   

  1. 新疆医科大学第一附属医院口腔正畸科, 新疆维吾尔自治区乌鲁木齐市   830054
  • 收稿日期:2010-11-08 修回日期:2010-12-08 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 米丛波，主任医师，硕士生导师，新疆医科大学第一附属医院口腔正畸科，新疆维吾尔自治区乌鲁木齐市830054 mi670105@sina.com
  • 作者简介:潘旭，女，1983年生，山东省平度市人，汉族，新疆医科大学第一附属医院口腔正畸科，专业方向为错牙合 畸形的防治。 panxu423@ hotmail.com
  • 基金资助:

    新疆医科大学第一附属医院科研项目青年专项基金(2009-QN-16)：正畸力作用下大鼠炎性牙周组织的改建及IL-6、8、11表达的研究。

Expression of interleukin-8 in periodontal tissue during experimental tooth movement in rats

Pan Xu, Mi Cong-bo, Liu Hong, Qian Ya-jing, Nie Jing   

  1. Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uighur Autonomous Region, China
  • Received:2010-11-08 Revised:2010-12-08 Online:2011-05-14 Published:2011-05-14
  • Contact: Mi Cong-bo, Chief physician, Master’s supervisor, Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uighur Autonomous Region, China mi670105@sina.com
  • About author:Pan Xu, Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uighur Autonomous Region, China panxu423@hotmail. com
  • Supported by:

    the Youth Foundation of Science and Technology Research of First Affiliated Hospital of Xinjiang Medical University*, No. 2009-QN-16*

PDF

314

被引次数

8

摘要：

背景：白细胞介素8作为一种与骨改建相关的炎性细胞因子，参与牙周组织的改建，促进牙槽骨的吸收，是骨吸收的标志物之一。
目的：观察正畸力对牙周组织改建过程中白细胞介素8表达的影响。
方法：将10周龄雄性SD大鼠上颌一侧第一磨牙与上颌切牙之间安置正畸装置，并施加0.49 N近中向正畸力，在加力后1，3，5，7，14 d取材，进行组织形态分析法、免疫组化染色检测白细胞介素8在牙周组织中表达的阳性细胞的面密度值。
结果与结论：正常大鼠牙周组织内白细胞介素8的表达呈弱阳性；随加力时间的延长大鼠牙周组织牙周膜细胞白细胞介素8表达增强，加力后5 d达到高峰，之后开始下降。实验提示正畸力可引起局部牙周组织炎症及白细胞介素8的释放。正畸牙移动早期，白细胞介素8的释放可能是牙周组织早期炎症反应和牙槽骨改建的触发因素之一。

关键词: 正畸力, 牙移动, 白细胞介素8, 免疫组化, 面密度值

Abstract:

BACKGROUND: Interleukin-8 (IL-8) as a bone remodeling associated with inflammatory cytokines, involved in periodontal tissue remodeling, and promote the absorption of alveolar bone, which is one marker of bone resorption.
OBJECTIVE: To observe the effects of orthodontic force on (IL-8) expression during periodontal tissue remodeling.
METHODS: The orthodontic devices were placed in maxillary first molars and maxillary incisors side of 10-week-old male SD rats, and impose 0.49 N orthodontic force. The specimen was harvested at 1, 3, 5, 7 and 14 days after operation and the surface density of IL-8 positive cells in periodontal tissues were determined by morphology analysis and immunohistochemical staining.
RESULTS AND CONCLUSION: The expression of IL-8 was observed at a low level in the control group. IL-8 expression increased after tooth movement and reached a peak at 5 days, after that, it gradually declined. Orthodontic force can cause local inflammation and periodontal tissue IL-8 release. IL-8 release may be an early inflammatory reaction in periodontal tissue and alveolar bone remodeling of the trigger factor at early stage of orthodontic tooth movement.

中图分类号: