中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3657-3661.doi: 10.3969/j.issn.1673-8225.2011.20.012

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脊髓损伤模型大鼠神经胶质细胞增殖与胶质纤维酸性蛋白的表达

李宽新1, 2，李  锋1    

  1. 1华中科技大学同济医学院附属同济医院骨科，湖北省武汉市430022
    2石河子大学医学院第一附属医院骨一科，新疆维吾尔自治区石河子市 832000
  • 收稿日期:2011-01-24 修回日期:2011-04-08 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 李锋，博士，主任医师，华中科技大学同济医学院附属同济医院骨科，湖北省武汉市 430022 lifeng6311@yahoo.com.cn
  • 作者简介:李宽新★，男，1969年生，安徽省凤阳县人，回族，2004年石河子大学医学院毕业，硕士，副主任医师，主要从事脊柱与关节外科研究。 likuanxin@sohu.com

Proliferation of glial cells and expression of glial fibrillary acidic protein after spinal cord injury in rats

Li Kuan-xin1, 2, Li Feng1   

  1. 1Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
    2First Department of Orthopedics, the First Affiliated Hospital of Shihezi University Medical School, Shihezi  832000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-01-24 Revised:2011-04-08 Online:2011-05-14 Published:2011-05-14
  • Contact: Li Feng, Chief physician Doctor, Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China lifeng6311@yahoo. com.cn
  • About author:Li Kuan-xin★, Master, Associate chief physician, Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; First Department of Orthopedics, the First Affiliated Hospital of Shihezi University Medical School, Shihezi 832000, Xinjiang Uygur Autonomous Region, China likuanxin@sohu.com

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被引次数

4

摘要：

背景：星形胶质细胞可以通过细胞裂解释放各种神经营养因子，并可促进损伤脊髓的修复。
目的：观察脊髓损伤模型大鼠神经胶质纤维酸性蛋白的表达及对其后肢功能恢复的影响。
方法：将SD大鼠采用Allen's法撞击T9~10节段致脊髓损伤，造模成功后蛛网膜下腔移植骨形态发生蛋白7，并设置仅蛛网膜下腔移植His蛋白的正常SD大鼠做对照。用BBB评分法评估两组大鼠后肢的运动功能，用免疫组织化学染色法和Western-blot法观察各组神经胶质纤维酸性蛋白的表达。
结果与结论：BBB评分结果显示，模型组大鼠脊髓损伤后下肢功能自行恢复率达68%。模型组脊髓损伤3和7 d，损伤区域神经胶质纤维酸性蛋白表达逐渐增加(P  < 0.05)，随后逐渐下降，于脊髓损伤28 d后逐渐恢复到对照组水平(P > 0.05)。脊髓损伤后1~14 d两组胶质纤维酸性蛋白表达逐渐升高(P > 0.05)。结果证实，脊髓损伤后蛛网膜下腔移植骨形态发生蛋白7可诱导星形胶质细胞增殖，神经胶质纤维酸性蛋白的表达增强，进而促进脊髓损伤大鼠后肢功能的恢复。

关键词: 胶质纤维酸性蛋白, 脊髓损伤, 骨形态发生蛋白7, BBB评分, 免疫组织化学, 组织构建

Abstract:

BACKGROUND: Astrocytes can release various neurotrophic factors by cell lysis to promote spinal cord repair.
OBJECTIVE: To observe the recovery of hjndlimb function and the expression of glial fibrillary acidic protein (GFAP) after spinal cord injury.
METHODS: Spinal cord injury models at T9-10 were established with Allen’s method. After modeling, bone morphogenetic protein 7 was injected into the subarachnoid space. Normal SD rats with His protein injected into the subarachnoid space were used as controls. The behavioral evaluation was performed with the Basso, Beattie & Bresnahanlocomotor rating scale (BBB scale). GFAP expression was detected using hematoxylin-eosin staining and immunohistochemistry staining. 
RESULTS AND CONCLUSION: Based on BBB score, the hindlimb function of rats in the model group recovered 68%. GFAP expression in the model group increased gradually at 3 and 7 days after injury (P < 0.05), and then decreased similar to the control group at 28 days after injury (P > 0.05). The data indicated that injection of bone morphogenetic protein 7 into the subarachnoid space can induce the proliferation of glial cells, increase GFAP expression and then to promote the recovery of hindlimb function after spinal cord injury in rats.

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