中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (20): 3625-3628.doi: 10.3969/j.issn.1673-8225.2011.20.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

应力缺失致骨质疏松大鼠成骨细胞细胞信号外调节激酶表达及通络生骨胶囊的影响

刘少军1，张  娴2，冯丽敏2，陈小俊1   

  1. 1广州中医药大学第一临床医学院关节一科，广东省广州市 510407
    2 广州中医药大学第二附属医院中心实验室，广东省广州市 510006
  • 收稿日期:2010-11-02 修回日期:2010-12-29 出版日期:2011-05-14 发布日期:2011-05-14
  • 通讯作者: 刘少军，广州中医药大学第一临床医学院关节一科，广东省广州市 510407 lioshaojun@163.net
  • 作者简介:刘少军☆，博士，副主任医师，硕士研究生导师，主要从事骨病方面的研究。
  • 基金资助:

    广东省科技厅项目(2007B031407010)，“通络生骨胶囊对人骨髓基质干细胞力学介导成骨通道的影响”。

Stress absence effect on ERK42/44 expression and intervention of Tongluo Shenggu capsules

Liu Shao-jun1, Zhang Xian2, Feng Li-min2, Chen Xiao-jun1   

  1. 1First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou  510407, Guangdong Province, China
    2Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou  510006, Guangdong Province, China
  • Received:2010-11-02 Revised:2010-12-29 Online:2011-05-14 Published:2011-05-14
  • Contact: Liu Shao-jun, First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510407, Guangdong Province, China
  • About author:Liu Shao-jun☆, Doctor, Associate cheif physician, Master’s supervisor, First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510407, Guangdong Province, China lioshaojun@163.net
  • Supported by:

    the Science and Technolgy Commission of Guangdong Province, No. 2007B031407010*

PDF

270

被引次数

3

摘要：

背景：长期卧床患者和航天员应力缺失导致骨质疏松现象的发生，通络生骨胶囊具有促进骨生成的作用，可能干预应力缺失状态下骨质疏松的发生。
目的：观察应力缺失型骨质疏松动物细胞信号外调节激酶的变化以及通络生骨胶囊对其改善作用。
方法：SD大鼠40只随机分为对照组、模型组、药物高、药物低剂量组，除正常组外以鼠尾悬吊法制备动物应力缺失模型，药物高、低剂量组将通络生骨胶囊按0.6，1.2 g/kg剂量灌胃，1次/d，21 d后，取腿骨，观察骨形态变化及成骨细胞细胞信号外调节激酶的表达水平。
结果与结论：应力缺失导致骨质疏松，对细胞信号外调节激酶总量无显著影响，但显著降低其磷酸化蛋白表达；通络生骨胶囊可显著改善应力造成的骨质疏松状态，但药物高剂量组对磷酸化的细胞信号外调节激酶的表达影响不显著，药物低剂量组则还降低其表达。说明应力缺失通过降低细胞信号外调节激酶磷酸化水平导致骨质疏松，通络生骨胶囊不是通过影响细胞信号外调节激酶通路改善应力缺失导致的骨质疏松的。

关键词: 应力, 细胞信号外调节激酶, 骨质疏松, 通络生骨胶囊, 信号传导, 成骨细胞

Abstract:

BACKGROUND: Stress absence induces osteoporosis in bedridden patients or astronauts. Tongluo Shenggu capsules can promote bone formation, which may affect stress absence-induced osteoporosis.
OBJECTIVE: To investigate influence of stress absence on the expression of ERK42/44, and intervention of the Tongluo Shenggu capsules.
METHODS: Totally 40 SD rats were randomly divided into normal control, model and high- and low-drug groups. Exception rats in the normal control group, all animals in other three groups were subjected to tail suspension test for preparing for stress absence models. Animals in the drug groups were administered Tongluo Shenggu capsule (0.6 or1.2 g/kg) once per day. After model preparation, animals were sacrificed, and bones of lower leg was taken out. Bone The norphological changes and expression of ERK was determined by western blot.
RESULTS AND CONCLUSION: Stress absence induced osteoporosis and decreased the amount of phosphorylation ERK, but had little effect on the total ERK. Low-dose of Tongluo Shenggu capsules decreased the expression of ERK phosphorylation, but high-dose of drugs had marginal on ERK. Stress absence induced osteoporosis by decreasing the phosphorylation of ERK, and the bone formation improving effect of Tongluo Shenggu capsules is not by regulating ERK expression.

中图分类号: