中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3534-3537.doi: 10.3969/j.issn.1673-8225.2011.19.027

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

嗅鞘细胞移植脊髓全横断损伤大鼠大脑皮质运动区转化生长因子β表达的影响

李  云1，彭  春2，王廷华3   

  1. 1大理学院附属医院神经内科，云南省大理州   671000
    2大理州公安局刑侦支队技术大队，云南省大理州 671000
    3昆明医学院神经科学研究所，云南省昆明市650031
  • 收稿日期:2010-11-20 修回日期:2011-01-19 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 王廷华，博士，教授，博士生导师，昆明医学院神经科学研究所，云南省昆明市 650031 tinghua_neuron@263.net
  • 作者简介:李云☆，女，1974年生，云南省大理州人，彝族，主治医师，2010年昆明医学院毕业，博士，主要从事脊髓损伤和脑血管病方面的研究。 dlhliyun@163.com

Effect of olfactory ensheathing cells transplantation on the expression of transforming growth factor beta in the motor cortex of rats subjected to spinal cord transection

Li Yun1, Peng Chun2, Wang Ting-hua3   

  1. 1Department of Neurology, the Affiliated Hospital of Dali University, Dali  671000, Yunnan Province, China
    2Department of Technology, the Public Security Bureau of Dali Prefecture, Dali  671000, Yunnan Province, China
    3Institute of Neuroscience, Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2010-11-20 Revised:2011-01-19 Online:2011-05-07 Published:2011-05-07
  • Contact: Wang Ting-hua, Doctor, Professor, Doctoral supervisor, Institute of Neuroscience, Kunming Medical University, Kunming 650031, Yunnan Province, China tinghua_neuron@263.net
  • About author:Li Yun☆, Doctor, Attending physician, Department of Neurology, the Affiliated Hospital of Dali University, Dali 671000, Yunnan Province, China dlhliyun@163.com

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摘要：

背景：多项研究已证实嗅鞘细胞能促进脊髓损伤大鼠神经功能的恢复，但其分子机制还不清楚。
目的：观察嗅鞘细胞移植对脊髓全横断大鼠大脑皮质运动区转化生长因子β mRNA表达的影响。
方法：采用酶消化法培养GFP转基因小鼠嗅鞘细胞，制成细胞悬液。建立SD大鼠T9脊髓全横断模型，造模后分为假手术组、模型组和嗅鞘细胞移植组。应用RT-PCR方法检测各组大鼠大脑皮质运动区转化生长因子β mRNA的表达变化，用β-actin作内参。
结果与结论：模型组大鼠造模后3 d大脑皮质运动区转化生长因子β mRNA的表达量高于假手术组(P < 0.05)，造模后7，14，21和28 d回到假手术组水平。嗅鞘细胞移植后21 d，嗅鞘细胞移植组转化生长因子β mRNA的表达量低于模型组(P < 0.05)。提示全横断脊髓损伤致大脑皮质运动区转化生长因子β mRNA早期表达上调，随后与假手术组相比无差别；嗅鞘细胞移植后期可逆转转化生长因子β mRNA的表达变化，有助于脊髓损伤的恢复。

关键词: 全横断脊髓损伤, 嗅鞘细胞, 细胞移植, 皮质运动区, 转化生长因子&beta

Abstract:

BACKGROUND: Many studies have showed that olfactory ensheathing cells (OECs) transplantation can result in significant functional improvements in rats subjected to spinal cord injury, but the underlying molecular mechanisms is not unknown.
OBJECTIVE: To observe the effect of OECs transplantation on the expression of transforming growth factor beta (TGF-β) mRNA in the motor cortex of the rats subjected to spinal cord transection.
METHODS: OECs isolated from transgenic mice expressing GFP and cultured by enzymatic digestion were made into cell suspension. Model of spinal cord transection (SCT) was established by cut transversely Sprague-Dawley (SD) rats T9 segment. After modeling, the rats were divided into sham-operation group, operation group and OECs transplantation group. RT-PCR was employed to detect gene expression of TGF-β. β-actin was used as control.
RESULTS AND CONCLUSION: RT-PCR results showed that compared with sham-operation group, the gene expression of TGF-β in the motor cortex increased significantly at 3 days postoperatively in the operation group (P < 0.05), and it returned to the level of sham-operation group at 7, 14, 21, and 28 days after modeling. Following OECs transplanatation, the expression of TGF-β in motor cortex at 21 days postoperatively showed a significant decrease compared with the operation group (P < 0.05). The findings indicate that spinal cord transection results in the up-regulation of the expression of TGF-β mRNA in the motor cortex in early period. OECs engrafted can decrease the expression of TGF-β mRNA in the later time so as to promote the recovery of spinal cord injury.

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