中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3494-3498.doi: 10.3969/j.issn.1673-8225.2011.19.017

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞经核转录因子κB途径干预心肌纤维化

邓  玮1，陈庆伟1，李兴升1，李桂琼1，柯大智1，莫显刚1，昝利萍2   

  1. 1重庆医科大学附属第二医院老年病科，重庆市   400010
    2重庆医科大学附属第一医院内分泌内科，重庆市  400016
  • 收稿日期:2010-10-08 修回日期:2010-11-13 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 陈庆伟，教授，博士生导师，重庆医科大学附属第二医院，重庆市 400010 chenqwcq@yahoo.com.cn
  • 作者简介:邓玮☆，女，1978年生，重庆市人，汉族，2011年重庆医科大学毕业，博士，主治医师，主要从事老年心血管病学研究。 dengwei1176@yahoo.com.cn
  • 基金资助:

    国家自然科学基金面上项目(30970826)资助。

Bone marrow mesenchymal stem cells prevent myocardial fibrosis via nuclear factor kappa B signaling pathway

Deng Wei1, Chen Qing-wei1, Li Xing-sheng1, Li Gui-qiong1, Ke Da-zhi1, Mo Xian-gang1, Zan Li-ping2   

  1. 1Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2Department of Endocrinology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
  • Received:2010-10-08 Revised:2010-11-13 Online:2011-05-07 Published:2011-05-07
  • Contact: Chen Qing-wei, Professor, Doctoral supervisor, Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China chenqwcq@yahoo.com.cn
  • About author:Deng Wei☆, Studying for doctorate, Attending physician, Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China dengwei1176@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30970826*

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摘要：

背景：骨髓间充质干细胞移植能否直接干预心肌纤维化及其可能的机制尚不完全清楚。
目的：观察骨髓间充质干细胞移植干预心肌纤维化的效果并分析其机制。
方法：分离培养雄性小鼠骨髓间充质干细胞，经尾静脉输入异丙肾性心肌纤维化雌性小鼠为治疗组，另设未治疗组和正常对照组。5周后处死小鼠，实时荧光定量PCR检测心肌y染色体鉴别基因(SRY)、基质金属蛋白酶9、基质金属蛋白酶组织抑制剂1的表达；天狼猩红染色对比心脏胶原纤维含量；免疫组织化学染色法观察心脏核转录因子κB表达。
结果与结论：骨髓间充质干细胞能归巢于纤维化心肌。与未治疗组相比，治疗组的心肌基质金属蛋白酶9、基质金属蛋白酶组织抑制剂1和核转录因子κB表达下调(P < 0.05)，胶原纤维含量下降(P < 0.05)。结果表明，骨髓间充质干细胞移植干预心肌纤维化，其机制可能与抑制核转录因子κB过度活化有关。

关键词: 心肌纤维化, 基质金属蛋白酶9, 基质金属蛋白酶组织抑制剂1, 核转录因子&kappa, B, 骨髓间充质干细胞

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) would show much help in treating myocardial fibrosis, and the mechanism involved remains unclear.
OBJECTIVE: To investigate the efficacy and mechanism of myocardial fibrosis intervention using BMSCs.
METHODS: BMSCs isolated from male mice were transfused into female mice with isoproterenol-induced myocardial fibrosis via tail vein (treated group). This study included three groups: treated, untreated, and control groups. Five weeks after transplantation, expression levels of sex-determining region of Y-chromosome (SRY), matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in myocardium were detected by fluorescent quantitative RT-PCR. Collagen distribution was observed using sirius red staining. The expression of nuclear factor-κB (NF-κB) was detected by immunohistochemistry.
RESULTS AND CONCLUSION: BMSCs can home to fibroid heart. Compared to the untreated group, treated group had decreased levels of MMP-9, TIMP-1, NF-κB and collagen deposition (P < 0.05). The results show that BMSCs transplantation can improve MMP-TIMP expression and collagen deposition in injured myocardium after homing, a mechanism that may be related to the NF-κB mediated signaling pathway.

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