中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3421-3426.doi: 10.3969/j.issn.1673-8225.2011.19.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

与软骨细胞共培养和条件培养液诱导骨髓间充质干细胞向软骨细胞分化的比较

吕  晶1，柯  杰1，王  琳2，张冬梅3，刘  冰1，逄键梁1，邓天政1   

  1. 1解放军空军总医院口腔科，北京市    100142
    2解放军总医院老年心血管病研究所，北京市  100853
    3解放军北京军区联勤部第二干休所门诊部口腔科，北京市  100071
  • 收稿日期:2010-12-06 修回日期:2011-01-29 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 邓天政，博士，主治医师，解放军空军总医院口腔科，北京市 100142 dengtzh@163.com
  • 作者简介:吕晶，女，1974年生，山东省巨野县人，达斡尔族，2000年解放军第四军医大学毕业，主治医师，主要从事口腔修复研究。 greatfriday@hotmail.com
  • 基金资助:

    国家自然科学基金，青年科学基金(30900314)“组织工程化骨-软骨组织构建过程中血管化与血管抑制的研究”；国家自然科学基金，青年科学基金(81000423)“下齿槽神经缺失对下颌骨牵张成骨影响的蛋白质组学分析”。

A comparative study on bone marrow mesenchymal stem cells differentiated into chondrocytes induced by co-culture with chondrocytes and conditioned medium

Lü jing1, Ke Jie1, Wang Lin2, Zhang Dong-mei3, Liu Bing1, Pang Jian-liang1, Deng Tian-zheng1   

  1. 1Department of Stomatology, the General Hospital of the Air Force, Beijing  100142, China
    2Institute of Geriatric Cardiology, Chinese PLA General Hospital, Beijing  100853, China
    3Department of Stomatology, Out-patient of the Second Retired Cadres, Logistics of Beijing Military Region, Beijing  100071, China
  • Received:2010-12-06 Revised:2011-01-29 Online:2011-05-07 Published:2011-05-07
  • Contact: Deng Tian-zheng, Doctor, Attending physician, Department of Stomatology, the General Hospital of the Air Force, Beijing 100142, China dengtzh@163.com
  • About author:Lü Jing, Attending physician, Department of Stomatology, the General Hospital of the Air Force, Beijing 100142, China greatfriday@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No.30900314*, 81000423*

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463

被引次数

31

摘要：

背景：骨髓间充质干细胞体外转化很大程度上依赖于合适的培养条件。
目的：比较与软骨细胞共培养和条件培养液2种不同的诱导方案诱导骨髓间充质干细胞向软骨细胞分化的特点。
方法：分离培养大鼠骨髓间充质干细胞和耳软骨细胞，采用骨髓间充质干细胞与软骨细胞共培养及条件培养液诱导成软骨的方法，诱导骨髓间充质干细胞向软骨细胞分化。以MTT法及流式细胞仪检测细胞活性及周期，糖胺多糖、甲苯胺蓝以及免疫组化染色检测细胞生物学特性，以RT-PCR法检测诱导后的软骨细胞Ⅱ型胶原RNA表达情况。
结果与结论：采用共培养方式诱导的软骨细胞，其生物学特性与采用条件培养液诱导的软骨细胞相比，前者优于后者，如分泌糖胺多糖的能力以及基质分泌量均较高。提示共培养方式诱导的软骨细胞更接近正常软骨细胞，更有利于作为组织工程软骨的种子细胞。

关键词: 共培养, 软骨细胞, 骨髓间充质干细胞, 诱导分化, 组织工程

Abstract:

BACKGROUND: In vitro differentiation of bone marrow mesenchymal stem cells (BMSCs) is dependent on appropriate culture conditions to a great extent.
OBJECTIVE: To study the biological characteristics of chondrocytes differentiated from BMSCs co-cultured with chondrocytes versus cultured in conditioned medium.
METHODS: Rat BMSCs and chondrocytes were isolated and cultured in neural induction. Cellular non-contact co-culture induced method and conditioned medium induced method were taken to reduce BMSCs differentiating into chondrocytes. And the biological characteristics of the cells were evaluated by MTT, mitotic cycle, GAG secretion, RT-PCR, cell-staining methods, immunocytochemistry.
RESULTS AND CONCLUSION: The biological characters of co-culture induced cells were seemed to be better than those of conditioned medium induced cells, which point out that BMSCs co-cultured with chondrocytes are appropriate as seed cells in cartilage tissue engineering.

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