中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1433-1436.doi: 10.3969/j.issn.1673-8225.2011.08.025

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

正交设计进行消淤接骨散的醇提工艺

周  钰1，黄国志1，梁东辉2，邓虹珠3，易延逵3，曾  庆1，吴卫卫1，樊  涛1   

  1. 南方医科大学附属珠江医院，1康复医学科，2中医科，广东省广州市  510282; 3南方医科大学中医药学院，广东省广州市 510515
  • 收稿日期:2010-08-02 修回日期:2010-09-25 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 梁东辉,主任医师、教授，南方医科大学附属珠江医院中医科，广东省广州市 510282 dhliang@hotmail.com
  • 作者简介:周钰★，女，1985年生 ，河南省驻马店市人，汉族，南方医科大学附属珠江医院康复医学科在读硕士，主要从事超声电导经皮透入纳米中药的镇痛机理和应用基础研究。 1061302248@qq.com
  • 基金资助:

    广东省科技计划项目（2006B35802005），课题名称：“纳米中药功能衬材的实验和应用研究”。

Ethanol extraction from connecting bones and reducing swelling powder by orthogonal design method

Zhou Yu1, Huang Guo-zhi1, Liang Dong-hui2, Deng Hong-zhu3, Yi Yan-kui3, Zeng Qing1, Wu Wei-wei1, Fan Tao1   

  1. 1Department of Rehabilitation Medicine, 2Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou   510282, Guangdong Province, China; 3College of Traditional Chinese Medicine, Southern Medical University, Guangzhou   510515, Guangdong Province, China
  • Received:2010-08-02 Revised:2010-09-25 Online:2011-02-19 Published:2011-02-19
  • Contact: Liang Dong-hui, Chief physician, Professor, Department of Traditional Chinese Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China dhliang@hotmail.com
  • About author:Zhou Yu★, Studying for master’s degree, Department of Rehabilitation Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China 1061302248@qq.com
  • Supported by:

    Science and Technology Plan of Guangdong Province, No. 2006B35802005*

PDF

323

被引次数

4

摘要：

背景：采用传统水提法提取的消淤接骨散药效不明显，推测可能为有效成分提取不充分所致。
目的：正交实验进行消淤接骨散醇提取工艺设计。
方法：以处方的醇浸膏得率和君药骨碎补有效成分柚皮苷含量为考察指标，采用L9(34)正交实验法进行优选，HPLC法测定柚皮苷含量，对消淤接骨散醇提取工艺进行优化筛选。观察醇浸膏得率，柚皮苷含量及吸醇率。
结果与结论：第3次醇提的醇浸膏得率及柚皮苷提取量所占比率均大于10%，应进行3次醇提方可完全提取药物成分。药材吸醇率平均值为265.67 mL/g，表明提取时第1次应多加3倍量溶剂。正交实验结果分析，由极差R值大小显示，各因素作用主次为加醇量>乙醇体积分数>提取时间，方差分析结果表明：乙醇体积分数、加醇量两因素的影响具有显著性意义(P < 0.05)。最佳优选工艺即药材加乙醇回流提取3次，第1次加6倍量体积分数65%乙醇提取2 h，第2次加5倍量体积分数65%醇提取1.5 h，第3次加5倍量体积分数65%醇提取1.5 h。较以往中药提取工艺可更有效得出药物有效成分、节省不必要的工艺流程，且该工艺操作简单，重复性好，柚皮苷含量高。

关键词: 消淤接骨散, 正交设计, 柚皮苷, 醇浸膏得率, 提取工艺

Abstract:

BACKGROUND: The drug effect does not show apparently through the method of aqueous extraction for the connecting bones and reducing swelling powder. The reason could be that the effective components cannot be extracted sufficiently.
OBJECTIVE: To optimize the ethanol extraction technology from the powder of connecting bones and reducing swelling by orthogonal trial.
METHODS: The ethanol extraction rate in prescription and Naringin volume, which is an effective component of principal drug rhizoma drynariae, were taken as the measurement index, for the optimization through L9(34) orthogonal trial. Naringin content was determined by high-performance liquid chromatography method and the ethanol extraction techniques from connecting bones and reducing swelling powder were optimized. The ethanol extraction rate, Naringin volume, and the rate of alcohol absorption were observed
RESULTS AND CONCLUSION: The percentages of ethanol extraction rate and Naringin volume were more than 10% in the third ethanol extraction. The components of Chinese herbal medicine can be completely extracted through three ethanol extractions. The average of alcohol absorption rate was 265.67 mL/g, which means more than 3 times of menstruum would be added in the first ethanol extraction. The result of orthogonal test showed that the dosage of menstruum was the key factor, the ethanol volume fraction was the subordination factor and the time cost in extraction was the most subordination factor, assayed by range R value. The analysis of variance showed that there were significant differences in the effect of the ethanol volume fraction and the dosage of menstruum (P < 0.05), so the optimized extraction condition was: 65% ethanol refluxed and extracted for three times, six times of ethanol extracted for 2 hours, five times for 1.5 hours, five times for 1.5 hours respectively. The optimized extraction technique is superior to routine technology regarding extracting drug components, thus avoiding unwanted technology process. The optimized process is repeatable, simply operated and could reach high naringin content.

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