中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6785-6789.doi: 10.3969/j.issn.1673-8225.2010.36.030

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

扫描电镜显示人胎肝和肝癌细胞表面微绒毛样突起与胞外膜粒及隧道纳米管的关系

赵  翔1，胡白和2，赵新荣2，张  页1   

  1. 北京大学，1基础医学院细胞生物学系，2医药卫生分析中心电镜室，北京市  100191
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 张页，副教授，北京大学基础医学院细胞生物学系，北京市 100191 zhangye@bjmu.edu.cn
  • 作者简介:赵翔，女，北京市人，汉族，主管技师，主要从事肿瘤分子细胞生物学研究。 xiangzh@bjmu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目(30570413)和北京大学985细胞生物学重点学科建设项目。

Relationship of microvillus-like protrusions to extracellular membrane particles and tunneling nanotubes on the surfaces of human fetal and hepatocellular carcinoma cells revealed by a scanning electron microscope

Zhao Xiang1, Hu Bai-he2, Zhao Xin-rong2, Zhang Ye1   

  1. 1 Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing  100191, China; 2 Electron Microscope Laboratory, Health and Medical Analysis Center, Peking University, Beijing  100191, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Zhang Ye, Associate professor, Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing 100191, China zhangye@bjmu.edu.cn
  • About author:Zhao Xiang, Technician-in-charge, Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing 100191, China xiangzh@bjmu.edu. cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30570413; the Key Subject Construction Project of 985 Cell Biology of Peking University

PDF

533

被引次数

3

摘要：

背景：肝脏来源的胞外膜粒可介导细胞间通讯、调控肝细胞的增殖与分化，在肝病诊断、肝癌防治、肝脏再生和细胞移植等技术的研究应用中均有重要价值。但由于对膜粒的发生和类型归属缺乏认识，制约了该领域的进展。隧道纳米管是介导细胞间通讯的另一新发现的结构，是否存在于肝细胞间尚无报道。
目的：通过对比人正常胎肝细胞和肝癌细胞表面超微结构的特点，寻找两种细胞的差异，揭示胞外膜粒的生物发生过程，判断其类型归属，明确隧道纳米管是否存在。
方法：用扫描电镜观察处于增殖周期中的贴壁培养胎肝L02和肝癌BEL-7402细胞样品，分析其细胞表面超微结构的共性和差异。
结果与结论：首次显示人胎肝L02和肝癌BEL-7402细胞表面均具有大量微绒毛样突起，其特征与静止期细胞的典型微绒毛有显著差异。L02细胞上微绒毛样突起的平均长度和密度均超过BEL-7402细胞，可作为区分两种细胞的指标。两种细胞上的微绒毛样突起均既可产生纳米膜粒，又可转化为隧道纳米管。纳米膜粒的产生方式有出芽和串珠样缢缩两种类型。总之，高分辨率扫描电镜图像可作为鉴定肝脏来源细胞表面超微结构特征和胞外膜粒来源的金标准。

关键词: 扫描电镜, 微绒毛, 胞外膜粒, 隧道纳米管, 胎肝细胞, 肝癌细胞

Abstract:

BACKGROUND: Liver-derived extracellular membrane particles can mediate cell-cell communication and regulate proliferation/differentiation of hepatocytes, and therefore have important application values in diagnosis of liver diseases, in prevention and treatment of liver cancers, and in development of techniques for liver regeneration and cell transplantation. However, progress in the field is hampered by limited knowledge about the origination and typological classification of liver-derived membrane particles. Tunneling nanotube (TNT) is anther newly discovered structure that also mediates cell-cell communication. However, the question whether TNT exists in hepatocytes remains unanswered.
OBJECTIVE: By comparing ultrastructural characteristics of the cell surfaces of human normal fetal liver and hepatocellular carcinoma cells, the study is aimed to find the differences between normal and cancerous cells, to uncover the biogenesis of membrane particles so as to accurately classify the particles, and to assure the existence of TNT in liver cells.
METHODS: A scanning electron microscope (SEM) was used to probe the samples prepared from cycling adhered fetal hepatocytes L02 and hepatocellular carcinoma BEL-7402 cells to analyze the commonness and difference of cell surface ultrastructure.
RESULTS AND CONCLUSION: Extensive microvillus-like protrusions were revealed for the first time on the cell surfaces of both L02 and BEL-7402 cells. The protrusions were characteristically different with the typical microvilli on resting cells. Compared with the BEL-7402 cells, however, the average length and density of the microvillus-like protrusions were longer and higher on the L02 cells, which could be used as a guide to differentiate the two cells. Microvillus-like protrusions from the two cells could either generate nanoparticles or transform into TNT. Two types of nanoparticle biogenesis, budding and pearl-forming constriction, were observed. Taken together, high-resolution SEM images can be served as gold standard to characterize the surface ultrastructures on liver-derived cells, and to pinpoint the sources of extracellular membrane particles.

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