中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6672-6676.doi: 10.3969/j.issn.1673-8225.2010.36.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质样干细胞和骨髓单个核细胞影响博莱霉素诱导肺纤维化的比较

陈  娟1，刘薇薇2，刘移民2，李素梅1，余  卫2，陈嘉榆2，张  成2，林琛莅1，唐丽娟1，方  茅1，钟雪云1   

  1. 1暨南大学医学院病理学教研室，广东省广州市  510632；2广东省广州市第十二人民医院，广东省广州市  510632
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 钟雪云，博士，教授，博士生导师，暨南大学医学院病理学教研室，广东省广州市 510632 Tzxy@jnu.edu.cn
  • 作者简介:陈娟★，女，1986年生，湖南省隆回县人，汉族，暨南大学在读硕士，主要从事病理学方面的研究。 chenjuan851229@163.com
  • 基金资助:

    广州市医药卫生科技项目(编号：2008-ZDi-04），项目名称：Smad7和HGF基因在矽肺纤维化的作用及靶向治疗的研究。

Effect of bone marrow-derived mesenchymal-like stem cells versus bone marrow-derived mononuclear cells on bleomycin-induced pulmonary fibrosis

Chen Juan1, Liu Wei-wei 2, Liu Yi-min2, Li Su-mei1, Yu Wei2, Chen Jia-yu2, Zhang Cheng2, Lin Chen-li1, Tang Li-juan1, Fang Mao1,Zhong Xue-yun1   

  1. 1 Department of Pathology, Medical College of Jinan University, Guangzhou   510632, Guangdong Province, China; 2 Guangzhou 12th People’s Hospital, Guangzhou  510632, Guangdong Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Zhong Xue-yun, Doctor, Professor, Doctoral supervisor, Department of Pathology, Medical College of Jinan University, Guangzhou 510632,Guangdong Province,China Tzxy@jnu.edu.cn
  • About author:Chen Juan★, Studying for master’s degree, Department of Pathology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China chenjuan851229@ 163.com
  • Supported by:

    the Medical Science and  Technology Program of Guangzhou City, No. 2008-ZDi-04

PDF

479

被引次数

8

摘要：

背景：有报道指出，骨髓间充质样干细胞和骨髓单个核细胞在多种疾病中被证明有疗效，骨髓间充质样干细胞在肺纤维化模型中也有应用。
目的:比较骨髓间充质样干细胞和骨髓单个核细胞对博莱霉素A5所致大鼠肺泡炎和早期纤维化的治疗作用。
方法:收集Wistar雄性幼鼠的骨髓间充质样干细胞、骨髓单个核细胞并分别进行DAPI标记。21只Wistar雌性大鼠气管内注入博莱霉素A5制作肺损伤模型，随机均分为骨髓间充质样干细胞治疗组、骨髓单个核细胞治疗组和模型组。造模后第7天处死大鼠，取肺组织病理切片观察炎症和纤维化情况；荧光显微镜下检测DAPI标记细胞；ELISA法检测肺组织匀浆羟脯氨酸和肿瘤坏死因子的含量。
结果与结论: ①在骨髓间充质样干细胞治疗组、骨髓单个核细胞治疗组肺组织冰冻切片中均见到DAPI标记的外源细胞。②模型组肺泡炎最严重，骨髓间充质样干细胞治疗组肺泡炎最轻，各组比较，差异有显著性意义(P < 0.05)。③模型组肺组织匀浆中羟脯氨酸和肿瘤坏死因子α含量最多，骨髓间充质样干细胞治疗最少，各组比较差异有显著性意义(P < 0.05)。提示骨髓间充质样干细胞和骨髓单个核细胞均可定植于损伤肺组织，并能有效阻止博莱霉素A5诱导的大鼠肺泡炎和早期纤维化，前者作用更为显著。

关键词: 骨髓单个核细胞, 骨髓间充质样干细胞, 细胞移植, 肺纤维化, 肿瘤坏死因子&alpha

Abstract:

BACKGROUND: Previous studies demonstrated that many diseases can be effectively treated by using both bone marrow-derived mesenchymal-like stem cells (BM-MSCs) and bone marrow-derived mononuclear cells (BM-MNCs). BM-MSCs also have been used in lung fibrosis models. 
OBJECIIVE: To compare the curative effect of BM-MSCs and BM-MNCs for treating bleomycin A5-induced alveolitis and early fibrosis.
METHODS: BM-MSCs and BM-MNCs from Wistar male young rats were cultured and marked with 4, 6-diamidino-2-phenylindole (DAPI). A total of 21 female Wistar rats were intratracheally injected with bleomycin A5 to establish pulmonary fibrosis models. The rats were randomly divided into BM-MSCs group, BM-MNCs group and model group. The rats were sacrificed on day 7 following model induction. Pathological sections of pulmonary tissues were obtained to observe inflammation and fibrosis. Fluorocyte marked with DAPI was detected by fluorescence microscopy. The levels of hydroxyproline and tumor necrosis factors-α (TNF-α) were measured by enzyme linked immunosorbent assay (ELISA).  
RESULTS AND CONCLUSION: ①In BM-MSCs group and BM-MNCs group, BM-MSCs and BM-MNCs marked with DAPI were detected in lung frozen section. ②Pulmonary alveolitis was most serious in model group, pulmonary alveolitis and fibrosis in BM-MNCs group was more serious than BM-MSCs group, and significant differences were detected among groups (P < 0.05). ③Hydroxyproline and TNF-α levels were highest in model group, and lowest in the BM-MSCs group, and significant differences were detected among groups (P < 0.05). Results suggest that both BM-MSCs and BM-MNCs can be planted in the impaired lung tissue, and effectively prevent the development of bleomycin-induced pulmonary alveolitis and early fibrosis. The curative effect of BM-MSCs is better than that of BM-MNCs.

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