中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 5923-5926.doi: 10.3969/j.issn.1673-8225.2010.32.007

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

骨髓基质细胞对多细胞因子介导人脐血单个核细胞体外扩增的影响

许  力1，毛  平2   

  1. 1广西壮族自治区人民医院血液科， 广西壮族自治区南宁市  530021； 2广州医学院附属广州市第一人民医院血液科，广东省广州市  510180
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 作者简介:许 力★，男，1973年生，安徽省淮北市人，汉族，2006年广州医学院毕业，硕士，主治医师，主要从事恶性血液疾病的诊断和治疗，造血干细胞移植方面的研究。 xuli943119@163.com
  • 基金资助:

    广西壮族自治区卫生厅课题(Z2008068)。

Effect of human bone marrow stromal cells in combination with several cytokines on in vitro amplification of mononuclear cells derived from umbilical cord blood  

Xu Li1, Mao Ping2   

  1. 1 Department of Hematology, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Department of Hematology, Guangzhou First People’ s Hospital of Guangzhou Medical College, Guangzhou  510180, Guangdong Province, China
  • Online:2010-08-06 Published:2010-08-06
  • About author:Xu Li★, Master, Attending physician, Department of Hematology, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Subject of Health Department of Guangxi Zhuang Autonomous Region, No. Z2008068*

PDF

327

被引次数

3

摘要：

背景：体外扩增脐血造血细胞的目的是促进脐血造血细胞的植入能力，细胞因子介导的脐血造血细胞能使细胞数有效扩增，但同时也耗竭了具有分化潜能的造血干细胞。
目的：观察骨髓基质细胞对多细胞因子组合介导人脐血单个核细胞体外扩增及扩增后黏附分子和CXCR4表达的影响。
方法：将分离的人脐血单个核细胞接种在预先建立的人骨髓基质细胞层上，分组培养：对照组仅含有脐血单个核细胞；多种细胞因子+脐血单个核细胞组；骨髓基质细胞+脐血单个核细胞组；骨髓基质细胞+多种细胞因子+脐血单个核细胞组。培养0，7 d检测有核细胞数, CD34+细胞数及CD34+CXCR4+ ，CD34+CD62L+ ，CD34+CD49d+的细胞数。
结果与结论：单纯骨髓基质细胞和单用细胞因子组均可有效地扩增脐血造血细胞，并提高造血细胞上CD49d,CD62L及 CXCR4表达。而单用细胞因子组促进脐血造血细胞扩增能力较单纯骨髓基质细胞强，提高造血细胞CD49d,CD62L及 CXCR4表达能力较单纯骨髓基质细胞差。提示骨髓基质细胞虽可支持脐血造血细胞扩增，但难以实现造血细胞的大量扩增，但在骨髓基质细胞层的支持下，多细胞因子可有效地促进脐血造血细胞的扩增，并优于单用细胞因子及骨髓基质细胞，证实骨髓基质细胞可协同多细胞因子有效地促进脐血单个核细胞的扩增，促进造血干细胞的黏附和趋化能力。

关键词: 脐血, 骨髓基质细胞, 单个核细胞, 体外扩增, CD49d, CXCR4

Abstract:

BACKGROUND: The goal of in vitro amplification of umbilical cord hematopoietic cells is promoting the engrafting capacity of umbilical cord hematopoietic cells, and the cytokine mediating can accelerate cell amplification, which exhausted the hemopoietic stem cells simultaneously.   
OBJECTIVE: To explore the effect of human bone marrow stromal cells (BMSCs) in combination with several cytokines on the in vitro expansion of the mononuclear cells derived from umbilical cord blood and the expression of adhesion molecule and CXCR4 after cell expansion.
METHODS: The isolated human mononuclear cells were incubated on BMSCs layers and cultured in 4 groups. Cells in the control group were cultured in single mononuclear cells; those in the other groups were cultured in several cytokines + mononuclear cells, BMSCs+ mononuclear cells, BMSCs+ several cytokines, and several cytokines + mononuclear cells, respectively. At 0 and 7 days after culture, the number of total nucleated cells, CD34+ cells, CD34+CXCR4+ , CD34+CD49d+ , and CD34+CD62L+ cells were counted.
RESULTS AND CONCLUSION: The single BMSCs and cytokine could effectively promote the amplification of umbilical cord hematopoietic cells and increased the expressions of CD49d, CD62L and CXCR4. The cell multiplication ability in the single cytokine group was greater than that in the BMSCs group, but poorly increased the expressions of CD49d, CD62L and CXCR4. It revealed that BMSCs can support the amplification of umbilical cord hematopoietic cells, but the supporting ability is limited. However, BMSCs in combination with several cytokines can effectively promote amplification of umbilical cord hematopoietic cells, the results of which is superior to the single cytokine or BMSCs. The results demonstrated that BMSCs in combination with several cytokines can enhance expansion of cord blood mononuclear cells, and promote the adhesion and chemotactic capacity of umbilical cord hematopoietic cells.

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