中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (29): 5479-5482.doi: 10.3969/j.issn.1673-8225.2010.29.040

• 生物材料综述 biomaterial review • 上一篇    下一篇

透明导电薄膜载体材料在无标记电化学基因芯片中的应用

吴回君1，罗丽琳2，张玉勤1   

  1. 1昆明理工大学材料科学与工程学院，云南省昆明市 650093；2云南省第一人民医院病理科，云南省昆明市 650032
  • 出版日期:2010-07-16 发布日期:2010-07-16
  • 通讯作者: 张玉勤，博士，教授，昆明理工大学材料科学与工程学院，云南省昆明市 650093 zyqkust@yahoo.com.cn
  • 作者简介:吴回君★，男，1983年生，浙江省台州市人，汉族，昆明理工大学在读硕士，主要从事生物陶瓷与生物医用材料的研究。 Whj520fly@163.com
  • 基金资助:

    云南省应用基础研究项目(2007E191M); 云南省教育厅科学研究基金项目(08J0011)。

Application of transparent conductive film carrier materials in label-free electrochemical biochip

Wu Hui-jun1, Luo Li-lin2, Zhang Yu-qin1   

  1. 1 School of Materials Science and Engineering, Kunming University of Science and Technology, Kunming   650093, Yunnan Province, China; 2 Department of Pathology, First People’s Hospital of Yunnan Province, Kunming   650032, Yunnan Province, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Zhang Yu-qin, Doctor, Professor, School of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China zyqkust@yahoo.com.cn
  • About author:Wu Hui-jun★, Studying for master’s degree, School of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China Whj520fly@163.com
  • Supported by:

    Applied Basic Research Program of Yunnan Province, No,2007E191M*; Scientific Research Fund of Yunan Province Education Bureau, No.08J0011*

PDF

519

被引次数

3

摘要：

背景：发展基于DNA杂交无标记电化学检测技术，是获得高灵敏度、强特异性、高可靠性、微型便携化及成本低廉电化学基因芯片的重要途径之一，而具有优异物理化学特性的新型载体材料对实现生物电信号在芯片系统中的高效稳定传递起着至关重要的影响。
目的：对无标记电化学基因芯片用透明导电氧化物薄膜载体材料的研究现状和进展进行了总结。
方法：应用计算机检索Elsevier全文数据库、APS全文数据库。资料检索时间为2001/2010。分次输入检索词检索文献，所有检索词为“Electrochemical DNA biochip/biosensor; Label-free electrical detection; Carrier materials; Transparent conductive oxide films”。纳入无标记电化学基因芯片领域中与透明导电氧化物薄膜载体材料等相关的文献。排除相关度不大和重复性文章。
结果与结论：透明导电氧化物薄膜经过功能化修饰后可以用作无标记电化学检测技术的电化学基因芯片新型载体材料。与其他类型的薄膜载体材料相比，其具有导电性能优异、化学稳定性高、生物相容性好及制备工艺简单等优点，是电化学基因芯片载体材料的理想选择之一。但是相关研究目前仍处于初级阶段，今后的重点方向是通过深入研究透明导电氧化物薄膜载体材料物化特性对生物电信号检测灵敏度、特异性及可靠性的影响及规律，弄清生物电信号在载体材料中及其与生物分子界面的电子传递机制。

关键词: 透明导电氧化物薄膜, 电化学基因芯片, 载体材料, 无标记电学检测, 综述文献

Abstract:

BACKGROUND: Based on DNA hybridization, the label-free electrical detection technology offers an important way to obtain a high sensitivity, strong specificity, high reliability, micro-portable and low-cost electrochemical DNA biochip. A carrier material with excellent physico-chemical characteristics plays a crucial role on highly efficient and stable transfer of the bioelectrical signals in the DNA biochip systems.
OBJECTIVE: To investigate and summarize the research progress in carrier materials of label-free electrochemical DNA biochip using transparent conductive oxide films, and to prospect its development trends.
METHODS: A computer-based online search of Elsevier and APS full-text databases was performed for articles and reviews published between 2001 and 2010 using the key words of “Electrochemical DNA biochip/biosensor; Label-free electrical detection; Carrier materials; Transparent conductive oxide films”. Researches about carrier materials of the label-free electrochemical DNA biochip using transparent conductive oxide films were included. Irrelevant or repetitive articles were excluded.
RESULTS AND CONCLUSION: Transparent conductive oxide films can be used as new carrier materials of the label-free electrochemical DNA biochip by functionalized modification. Compared with other types of the film carrier materials, it was one of the ideal carrier materials of the label-free electrochemical DNA biochip due to superior electrical properties, chemical stability, good biocompatibility, and simple preparation process. However, it was still in preliminary research, so future study should focus on the effects of physico-chemical characteristics of the transparent conductive oxide film carrier materials on detection sensitivity, specificity, reliability of the bioelectrical signals. As a result, the electron transfer mechanism of the bioelectrical signals on the carrier materials and their interface with biomolecules can be obtained.

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