中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (29): 5334-5338.doi: 10.3969/j.issn.1673-8225.2010.29.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

可注射性纤维蛋白凝胶/脱钙骨基质复合支架延缓兔骨关节炎软骨退变

何  劼，赵建宁，郭  亭   

  1. 解放军第二军医大学南京临床医学院，南京军区南京总医院，江苏省南京市  210002
  • 出版日期:2010-07-16 发布日期:2010-07-16
  • 通讯作者: 赵建宁，硕士，主任医师，教授，解放军第二军医大学南京临床医学院，南京军区南京总医院，江苏省南京市 210002 zhaojianning.0207@163.com
  • 作者简介:何劼☆，男，1982年生，江苏省滨海县人，汉族，解放军第二军医大学南京临床医学院，南京军区南京总医院骨科在读博士，主要从事关节外科研究。 Everest1982@gmail.com
  • 基金资助:

    全军“十一五”重点攻关项目（06G043）。

Injectable compound scaffold of fibrin gel/demineralized bone matrix to delay early osteoarthritis in rabbits

He Jie, Zhao Jian-ning, Guo Ting   

  1. Nanjing School of Clinical Medicine, Second Military Medical University of Chinese PLA, Nanjing General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing   210002, Jiangsu Province, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Zhao Jian-ning, Master, Chief physician, Professor, Nanjing School of Clinical Medicine, Second Military Medical University of Chinese PLA, Nanjing General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing 210002, Jiangsu Province, China zhaojianning.0207@163.com
  • About author:He Jie☆, Studying for doctorate, Nanjing School of Clinical Medicine, Second Military Medical University of Chinese PLA, Nanjing General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing 210002, Jiangsu Province, China Everest1982@gmail.com
  • Supported by:

    Key Medical Science and Technique Foundation of Chinese PLA during  the Eleventh Five-Year Plan Period, No. 06G043*

PDF

456

被引次数

4

摘要：

背景：纤维蛋白凝胶及脱钙骨基质都有优良的生物相容性及生物降解性，因此可以作为软骨组织工程支架培养软骨种子细胞。这两种材料分别可以作为生长因子的载体缓慢释放生长因子，并达到持续发挥促软骨细胞生长的功能。
目的：验证可注射性纤维蛋白凝胶/脱钙骨基质复合支架延缓骨性关节炎的可行性及有效性。
方法：实验为同体对照动物实验。16只兔切断双后肢前交叉韧带制备兔膝关节骨性关节炎模型，兔一侧膝关节内注射复合支架材料与软骨细胞混悬液为实验组。另一侧膝关节内注射等量生理盐水为对照组，12周后取关节软骨，分别进行苏木精-伊红染色，Masson染色，Ⅱ型胶原免疫组化染色，并根据Wakitani标准及Outerbridge分类重新制定标准进行评分，评价实验组与对照组软骨退变的程度。
结果与结论：实验组关节软骨表面尚光滑，可见软骨陷窝，结构软骨退变较对照组轻；软骨细胞及Ⅱ型胶原较正常软骨组织轻度减少，关节囊组织无明显Ⅱ型胶原表达。实验组综合评分低于对照组(P < 0.01)。表明可注射性纤维蛋白凝胶/脱钙骨基质复合支架为早期骨性关节炎及骨缺损修复提供了一种优良的复合支架材料。

关键词: 纤维蛋白凝胶, 脱钙骨基质, 骨关节炎, 软骨, 组织工程, 兔

Abstract:

BACKGROUND: Both fibrin gel and demineralized bone matrix can be used as cartilage tissue engineered scaffolds to culture cartilage seed cells, due to their good biocompatibility and biodegradablity. These materials can act as carriers for growth factors to slow release growth factors and to continuously promote chondrocyte growth.
OBJECTIVE: To verify the feasibility and efficacy of using injectable compound material made of fibrin glue and demineralized bone matrix as a scaffold to delay the progress of early osteoarthritis.
METHODS: A homobody controlled animal experiment was designed. Sixteen rabbits were cut off the anterior cruciate ligament from posterior limbs to prepare knee osteoarthritis model, one side of the knee joint in rabbits was injected with composite scaffold and chondrocyte suspension for the experimental group. The other side of the knee joint was injected with equal volume of saline as the control group. The articular cartilage was harvested 12 weeks later, for respectively hematoxylin-eosin staining, Masson staining, II collagen immunohistochemical staining. In accordance with Wakitani standards and Outerbridge classification, the standard score was redefined, the cartilage degenerations in the two groups were evaluated.
RESULTS AND CONCLUSION: In the experimental group, articular cartilage surface was smooth, cartilage lacuna could be seen, the structural cartilage degeneration was lighter than that in control group; chondrocyte and collagen type II were slightly less than normal cartilage, joint capsule tissue exhibited no expression of collagen type II. Comprehensive score of the experimental group was lower than the control group (P < 0.01). Injectable fibrin glue/demineralized bone matrix composite scaffold can provide a good composite scaffold for early osteoarthritis and bone defects.

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