中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 511-5194.doi: 10.3969/j.issn.1673-8225.2010.28.014

• 组织构建与生物活性因子 tissue construction and bioactive factors •    下一篇

外源性神经生长因子诱导下自体静脉桥接修复神经缺损

萧文耀，王  昕，段大鹏，王建民，丁轩玺，杨成业，陈根元，蒲兴海   

  1. 兰州大学第一医院骨科，甘肃省兰州市  730000
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 作者简介:萧文耀，男，1958年生，甘肃省兰州市人，回族，1983年兰州医学院毕业，副主任医师，主要从事关节外科，组织工程及运动损伤等相关研究。 doctorxinwang@163.com

Exogenous nerve growth factor-induced autologous vein bridge for repairing peripheral nerve defect

Xiao Wen-yao, Wang Xin, Duan Da-peng, Wang Jian-min, Din Xuan-xi, Yang Cheng-ye, Chen Gen-yuan, Pu Xing-hai   

  1. Department of Orthopedics, the First Hospital of Lanzhou University, Lanzhou   730000, Gansu Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Xiao Wen-yao, Associate chief physician, Department of Orthopedics, the First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China doctorxinwang@163.com

PDF

478

被引次数

18

摘要：

背景：采用自体神经游离移植修复神经缺损效果比较理想，但有其弊端。为此寻求一种更佳修复神经缺损的治疗方法。
目的：验证及外源性神经生长因子诱导下自体静脉桥接神经缺损对神经再生的影响。
方法：采用Wistar大鼠建立周围神经缺损模型。随机将大鼠分为3组。实验组采用自体静脉桥接并注入神经生长因子；对照组采用自体静脉桥接并注入生理盐水；标准组采用自体神经桥接。分别于术后1，3个月，对实验动物进行活体观察，电生理检测及组织学检测。
结果与结论：3组实验动物均有神经再生及修复表现，但程度不同。实验组失神经表现恢复的较对照组早，电生理检测运动神经传导速度快，组织学检查再生神经纤维数量及质量明显高于对照组(P < 0.05)；与“金标准”的自体神经桥接组比较无显著性意义(P > 0.05)。结果提示采用自体静脉桥接+神经生长因子诱导对周围神经缺损后的再生、修复具有有促进作用，可以使再生神经纤维的数量增加并显著提高再生神经纤维质量。

关键词: 自体静脉桥接, 神经生长因子, 神经缺损, 修复, 神经组织工程

Abstract:

BACKGROUND: It is relatively ideal to repair nerve defects using autologous nerve graft. But it has its drawbacks. A better treatment for repairing nerve defects should be found.
OBJECTIVE: To investigate the influence of autogenous vein graft (AVG) under induction of exogenous nerve growth factor (NGF) on the peripheral nerve repair and regeneration after injury.
METHODS: Wistar rats were used to prepare injured models of peripheral nerve and were divided randomly into three groups. The nerve defect was bridged with autogenous vein, and the neural regeneration room was formed and injected with NGF in the experimental group. The rats in the control group were injected with normal saline instead of NGF. In the standard group, autogenous nerve grafting was performed. The animal ethology observation, electrophysiological and morphological detection were performed at 1 and 3 months after operation.
RESULTS AND CONCLUSION: All experimental animals had different regeneration and repair of nerve tissues. By contrast, there was a better manifestation in repairing the peripheral nerve defects in the experimental group, and neuroelecrophysiology detection showed that nerve concluctive velocity (NCV) was significantly faster than those in the other groups. Morphological observation showed that the quantity and quality of regenerative nerve fiber were superior to the control group obviously (P < 0.05), and they had not significant differences compared with the standard group (P > 0.05). The co-therapy of NGF and autogenous vein graft can promote repair and regeneration of peripheral nerve after injury. The treatment not only can increase quantity of regenerative nerve fiber, but also can significantly promote their quality.

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