中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5159-5162.doi: 10.3969/j.issn.1673-8225.2010.28.007

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

高糖对心肌微血管内皮细胞表达神经调节蛋白1的影响

施国祥1，郑泽琪1，李宾公1，席海龙2，康  婷1，孙传福1   

  1. 1南昌大学第一附属医院心血管内科，江西省南昌市  330006；2江西省新余市人民医院心血管内科，江西省新余市 338025
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 郑泽琪，教授，主任医师，南昌大学第一附属医院心血管内科，江西省南昌市 330006 zzq620712@sina.com
  • 作者简介:施国祥★，男，1983年生，江西省余干县人，汉族，南昌大学在读硕士，主要从事心血管内科方面的研究。
  • 基金资助:

    国家自然科学基金(30860101)，课题名称：神经调节蛋白1转染脂肪来源的内皮祖细胞移植治疗糖尿病心肌病。

Effect of high glucose on the neuregulin-1 expression of cardiac microvascular endothelial cells  

Shi Guo-xiang1, Zheng Ze-qi1, Li Bin-gong1, Xi Hai-long2, Kang Ting1, Sun Chuan-fu1   

  1. 1 Department of Cardiology, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2 Department of Cardiology, Xinyu People's Hospital, Xinyu  338025, Jiangxi Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Zheng Ze-qi, Professor, Chief physician, Department of Cardiology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China zzq620712@sina.com
  • About author:Shi Guo-xiang★, Studying for master’s degree, Department of Cardiology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30860101*

PDF

381

被引次数

17

摘要：

背景：糖尿病心肌病的发病机制尚不清楚，神经调节蛋白1具有促进血管再生等作用，糖尿病心肌病的发病是否和心肌微血管内皮细胞表达神经调节蛋白1下降有关值得进一步研究。
目的：观察高糖对心肌微血管内皮细胞表达神经调节蛋白1的影响。
方法：取生长良好的第2代心肌微血管内皮细胞，高糖组加入10 mmol/L的葡萄糖，高糖+胰岛素组加入10 mmol/L的葡萄糖及10-5 U/L的胰岛素，对照组正常培养。培养24 h后收集细胞，RT-PCR及Western blot方法检测神经调节蛋白1 mRNA和蛋白的表达。
结果与结论：与对照组比较，高糖组心肌微血管内皮细胞神经调节蛋白1 mRNA和蛋白的表达明显下降(P < 0.05)；与高糖组比较，高糖+胰岛素组心肌微血管内皮细胞神经调节蛋白1 mRNA和蛋白的表达明显升高(P < 0.05)，与对照组间差异无显著性意义(P > 0.05)。说明高糖可抑制心肌微血管内皮细胞表达神经调节蛋白1 mRNA和蛋白，而胰岛素可拮抗此作用。

关键词: 神经调节蛋白1, 高糖, 胰岛素, 心肌微血管内皮细胞, 糖尿病心肌病

Abstract:

BACKGROUND: The pathogenesis of diabetic cardiomyopathy is unclear. Neuregulin-1 (NRG-1) can promote revascularization. Whether the incidence of diabetes cardiomyopathy is relevant to the down expression of NRG-1 of cardiac microvascular endothelial cells needs further research.
OBJECTIVE: To observe the effect of high-glucose on the expression of the NRG-1 of cardiac microvascular endothelial cells.
METHODS: The second generation of cardiac microvascular endothelial cells were selected and divided into three groups. Control group: cells were cultured in normal medium; high-glucose group: glucose (10 mmol/L) was added into medium;  glucose + insulin group: glucose (10 mmol/L) and insulin (10-5 U/L) were added into medium. Cardiac microvascular endothelial cells were collected after 24 hours, RT-PCR and Western blot were used to detect the expression of NRG-1 mRNA and protein. 
RESULTS AND CONCLUSION: Compared with the control group, the expression of the NRG-1 mRNA and NRG-1 protein in the high-glucose group were decreased significantly (P < 0.05). Compared with high-glucose group, the expression of the NRG-1 mRNA and NRG-1 protein in the glucose + insulin group were increased significantly (P < 0.05). Compared with the control group, the difference was not significant (P > 0.05). High glucose can inhibit the expression of NRG-1 mRNA and NRG-1 protein of cardiac microvascular endothelial cells, which can be antagonized by insulin.

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