中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4619-4622.doi: 10.3969/j.issn.1673-8225.2010.25.015

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微囊化异种坐骨神经组织细胞移植脊髓损伤大鼠核因子κB的表达

王小莲1，曾文泓2，王  婷1，刘德明3   

  1. 1 三峡大学医学院，湖北省宜昌市   443002；2江西中医学院，江西省南昌市  330006；   3南昌大学医学院，江西省南昌市   330006
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 通讯作者: 刘德明，教授，博士生导师，南昌大学医学院，江西省南昌市 330006 jxana@sina.com
  • 作者简介:王小莲★，1984年生，女，江西省吉安市人，汉族，2009年南昌大学毕业，硕士，助教，主要从事神经损伤修复与再生的研究。 wxiaolian@126.com
  • 基金资助:

    国家自然科学基金资助项目（30060034）。

Effects of xenotransplantation of microencapsulated sciatic nerve on the expression of nuclear factor-kappa B following spinal cord injury in rats

Wang Xiao-lian1, Zeng Wen-hong2, Wang Ting1, Liu De-ming3   

  1. 1 Medical College of Three Gorges University, Yichang  443002, Hubei Province, China; 2 Jiangxi University of Traditional Chinese Medicine, Nanchang  330006, Jiangxi Province, China; 3 Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Liu De-ming, Professor, Doctoral supervisor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China jxana@sina.com
  • About author:Wang Xiao-lian★, Master, Research assistant, Medical College of Three Gorges University, Yichang 443002, Hubei Province, China wxiaolian@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30060034*

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摘要：

背景：核因子κB是一种重要的转录因子，在炎症反应中起重要作用。
目的：观察微囊化异种坐骨神经组织细胞移植于脊髓损伤大鼠后核因子κB的表达及活性变化。
方法：家兔用于制备异种坐骨神经组织细胞悬液。SD大鼠120只随机被分为4组。假手术组，建立大鼠脊髓半横断伤模型后分微囊组、细胞组、单损组。于损伤处分别植入明胶海绵吸附的10 μL 微囊化异种坐骨神经组织细胞、明胶海绵吸附的10 μL 异种坐骨神经组织细胞以及明胶海绵吸附的10 μL生理盐水。术后6，12，24 h，3，7，14 d，苏木精-伊红染色观察脊髓组织的病理学改变及免疫组织化学染色观察核因子κB的表达情况。
结果与结论：脊髓损伤大鼠神经元细胞浆及细胞核内核因子κB表达增加，24 h达高峰水平，3 d后开始逐渐降低，7 d基本降至正常。微囊组脊髓核因子κB阳性细胞的体积密度和表面积密度值均少于单损组、细胞组(P < 0.05)。 结果提示，微囊化异种坐骨神经组织细胞移植对脊髓损伤后核因子κB的活性表达起抑制作用，有益于减轻核因子κB介导的炎性反应。

关键词: 微囊, 核因子-&kappa, B, 脊髓损伤, 异种移植, SD大鼠

Abstract:

BACKGROUND: Nuclear factor-κB (NF-κB) is an important transcription factor and plays an important role in response to inflammation.
OBJECTIVE: To investigate the effects of transplantation of microencapsulated rabbit sciatic nerve on NF-KB activity after spinal cord injury in rats．
METHODS: Rabbits were used to prepare xenosciatic nerve cell suspension. Totally 120 Sprague Dawley rats were randomly divided into four groups: sham operation, microcapsule, cell and single injury groups. Rats in the later three groups were prepared for hemisection injury of spinal cords and transplanted with gelatin sponge sticking 10 μL microencapsulated nerve tissue/cell suspension, 10 μL tissue/cell suspension or 10 μL physiological saline. The pathological changes of spinal tissues were observed by haematoxylin-eosin staining, and the expressions of NF-KB were observed by immunohistochemistry at 6, 12, 24 hours and 3, 7 and 14 days after operation.   
RESULTS AND CONCLUSION: The expressions of NF-κB in cytoplasm and cytoblast of neurons were increased in rats after spinal cord injury, reached a peak at 24 hours, gradually decreased after 3 days, and returned to the normal levels after 7 days. The expressions of NF-κB in the microcapsule group were statistically smaller than those in the cell and single injury groups (P < 0.05). The transplantation of microencapsulated rabbit sciatic nerve can inhibit expression of NF-KB and mitigate the inflammatory reaction after spinal cord injury in rats．

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