中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4607-4610.doi: 10.3969/j.issn.1673-8225.2010.25.012

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

纳米银猪脱细胞真皮敷料的细胞毒性评估

黄桂娟，景红霞，夏栋林，鲁双云，顾海鹰   

  1. 南通大学公共卫生学院生命分析化学研究所，江苏省南通市   226007
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 通讯作者: 鲁双云，硕士，讲师，南通大学公共卫生学院生命分析化学研究所，江苏省南通市 226007
  • 作者简介:黄桂娟★，女，1982年生，江苏省南通市人，汉族，南通大学在读硕士，主要从事生物组织工程与纳米电化学研究。 pdhgj@126.com
  • 基金资助:

    国家自然科学基金（20875051），课题名称：血红蛋白/Au@Fe3O4}n"多层组装的生物分子电子器件研究；国家自然科学基金（20675042），课题名称：基于“ {血红蛋白/纳米粒子（团簇）}n ”多层组装的生物纳米器件研究；江苏省自然科学基金（BK2009152），课题名称：Fe3O4磁性纳米粒子的表面功能化修饰、生物兼容性及其应用研究；南通市社会发展项目(s2008008)，课题名称：“角质形成细胞/成纤维细胞/猪皮支架”的构建及应用研究。

Cytotoxiciy of nano-silver/porcine acellular dermis dressings

Huang Gui-juan, Jing Hong-xia, Xia Dong-lin, Lu Shuang-yun, Gu Hai-ying   

  1. Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong   226007, Jiangsu Province, China
  • Online:2010-06-18 Published:2010-06-18
  • Contact: Lu Shuang-yun, Master, Lecturer; Gu Hai-ying, Doctor, Professor, Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong 226007, Jiangsu Province, China hygu@ntu.edu.cn; guhy99@21cn.com
  • About author:Huang Gui-juan★, Studying for master’s degree, Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong 226007, Jiangsu Province, China pdhgj@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 20875051*, 20675042*; the Natural Science Foundation of Jiangsu Province, No. BK2009152*; the Social Development Project of Nantong, No. s2008008*

PDF

457

被引次数

14

摘要：

背景：传统的猪皮敷料具有保护创面的作用，但不具有主动抗感染功能。
目的：评估自制纳米银猪脱细胞真皮敷料的体外细胞毒性，评价其作为新型敷料的可行性。
方法：用四甲基偶氮唑盐比色法检测自制纳米银猪脱细胞真皮敷料100%和50%的浸提液对小鼠成纤维细胞(L-929细胞)相对增殖率的影响，并评价其细胞毒性。倒置显微镜观察各组细胞形态变化，培养2，4，7d后用酶联免疫检测仪测定溶液吸光度值来评估细胞毒性。
结果与结论：L-929细胞在自制纳米银猪脱细胞真皮敷料的浸提液中增殖良好，其细胞毒性实验显示细胞毒级为1级，实验组与阴性对照组之间的吸光度值差异均无显著性意义( P >0.05)。说明自制纳米银猪脱细胞敷料无细胞毒性，作为新敷料具有可行性。

关键词: 猪脱细胞真皮基质, 敷料, 纳米银, 细胞毒性, MTT

Abstract:

BACKGROUND: Traditional porcine dermal dressing can protect wounds, but it has not the properties of bacteriostasis efficacy.
OBJECTIVE: To assess in vitro toxicity of self-made nano-silver porcine acellular dermal dressing and to evaluate the feasibility of a new type of dressing.
METHODS: L-929 mouse fibroblasts were used to detect the cell relative proliferation rate in the extract from the nano-silver porcine dermal dressing 100% and 50% by MTT-assay, the toxicity of porcine dressing was also determined. Morphology of the cells was observed under inverted microscope. The absorbance value was detected using enzyme-linked immunospot assay at 2, 4, 7 days of the culture, and the cytotoxicity was assessed.
RESULTS AND CONCLUSION: The L-929 cells proliferated well in the extract of nano-silver porcine acellular dermis dressing, and the cell toxicity was graded as 1. There was no significant difference in the absorbance value between the experimental group and the negative control group ( P > 0.05). The nano-silver pig acellular dermis dressing shows no evident cytotoxicity and is feasible to serve as a new type of wound dressing.

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