中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (25): 4581-4584.doi: 10.3969/j.issn.1673-8225.2010.25.006

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

壳聚糖-胶原凝胶复合骨髓间充质干细胞修复兔关节软骨缺损的组织学变化

于洪宇，马春雨   

  1. 辽宁医学院护理学院，辽宁省锦州市  121000
  • 出版日期:2010-06-18 发布日期:2010-06-18
  • 作者简介:于洪宇，女，1976年生，辽宁省锦州市人，汉族，2000年中国医科大学毕业，讲师，主要从事基础医学与护理学的研究。

Histological research of rabbit articular cartilage defects repaired by chitosan-collagen gel combined with bone marrow mesenchymal stem cells

Yu Hong-yu, Ma Chun-yu   

  1. School of Nursing, Liaoning Medical College, Jinzhou   121000, Liaoning Province, China
  • Online:2010-06-18 Published:2010-06-18
  • About author:Yu Hong-yu, Lecturer, School of Nursing, Liaoning Medical College, Jinzhou 121000, Liaoning Province, China

PDF

356

被引次数

24

摘要：

背景：大块软骨损伤目前临床上尚无方法治疗，干细胞技术出现后为解决这一难题提供了理论支持。采用新型的支架材料“壳聚糖-胶原蛋白”结合干细胞诱导分化移植给动物模型是一种较新的尝试。
目的：观察壳聚糖-胶原凝胶复合骨髓间充质干细胞修复兔关节软骨缺损的组织学变化。
方法：体外培养扩增兔骨髓间充质干细胞。采用软骨诱导分化培养基对P2代细胞进行成软骨诱导。同时制备“壳聚糖-胶原蛋白”支架和兔关节软骨缺损模型。缺损用含有骨髓间充质干细胞的壳聚糖-胶原凝胶填充，另一条关节用没有细胞的支架或不做处理。其中12个关节作为实验组(接受骨髓间充质干细胞+支架)，8个关节作为空白对照组(不做处理)，8个关节作为单纯支架组(未植入细胞)。造模后于2，4，8，16周进行组织学评分并处死动物行组织学染色。
结果与结论：造模后16周移植的细胞一致分化为软骨细胞，大部分软骨缺损区被新生软骨修复，组织学评分实验组关节修复良好。提示应用骨髓间充质干细胞结合“壳聚糖-胶原蛋白”复合物可以修复关节软骨缺损。

关键词: 壳聚糖-胶原凝胶, 兔, 骨髓间充质干细胞, 软骨缺损, 组织学变化

Abstract:

BACKGROUND: Large cartilage defects is a difficulty problem in clinical treatment, and stem cell techniques provide a theoretic support for this problem. It is a new attempt that applying novel scaffold chitosan-collagen protein with stem cells to transplant in animal models.
OBJECTIVE: To observe the histological changes of chitosan-collagen gel in combined with bone marrow mesenchymal stem cell for repairing rabbit articular cartilage defects.
METHODS: Rabbit bone marrow mesenchymal stem cells were cultured in vitro. P2 cells were differentiated in chondrogenic medium. Chitosan-collagen scaffold and rabbit articular cartilage defect models were produced. The defects were filled with chitosan-collagen gel scaffold containing bone marrow mesenchymal stem cells, while the other joint was treated with cell-free scaffold or received no treatment. There were 12 joints serving as experiment group (treated by bone marrow mesenchymal stem cells + scaffold), 8 joints as blank control group (with no treatment) and 8 joints as single scaffold group (with no cells). At 2, 4, 8, 16 weeks after the establishment of rabbit models, the rabbits were killed and then histological staining was done.
RESULTS AND CONCLUSION: At 16 weeks after the establishment of defect models, the transplanted cells differentiated into chondrocytes, a majority of cartilage defect areas were repaired by new cartilage, the histological scores showed good articular repair in experiment group. Bone marrow mesenchymal stem cells combined chitosan-collagen could repair articular cartilage defect.

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