中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4473-4476.doi: 10.3969/j.issn.1673-8225.2010.24.023

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

caspase-3/8 mRNA在增殖期病理性瘢痕组织中的表达

朱绍般1，刘达恩1，黎洪棉2，牛海艳3   

  1. 1广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市   530021；2 中山大学附属中山医院整形美容外科，广东省中山市   518403；3 南方医科大学病理学系，广东省广州市  510515
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 通讯作者: 刘达恩，主任医师，广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021 Liudaen@163.com
  • 作者简介:朱绍般★，男，1980年生，广西壮族自治区贵港市人，壮族，2009年广西医科大学研究生学院毕业，硕士，医师，主要从事难愈性创面和烧伤瘢痕方面的研究。 Shaoban303@163.com
  • 基金资助:

    广西科学基金资助项目(桂科自0728110)。

Expression of caspase-3/8 mRNA in hypertrophic scars

Zhu Shao-ban1, Liu Da-en1, Li Hong-mian2, Niu Hai-yan3   

  1. 1 Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Department of Plastic and Aesthetic Surgery, Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan  518403, Guangdong Province, China; 3 Department of Pathology, Southern Medical University,  Guangzhou   510515, Guangdong Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Liu Da-en, Chief physician, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liudaen@163.com
  • About author:Zhu Shao-ban★, Master, Physician, Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Shaoban303@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0728110*

PDF

293

被引次数

18

摘要：

背景：细胞凋亡参与了肉芽组织向瘢痕组织转化过程中多种细胞成分大量减少的动态过程，而Caspase家族介导的细胞凋亡是细胞凋亡的主要途径。
目的：从基因水平探讨caspase-3 mRNA及caspase-8 mRNA在增生性瘢痕组织中的作用。
方法：实验所用病理性瘢痕组织及正常皮肤组织均取自本院烧伤整形外科住院、伤口愈合6个月以后的增殖期病理性瘢痕组织18例。以正常皮肤组织18例做对照。利用RT-PCT技术分别检测半年以上增生性瘢痕组织中成纤维细胞caspase-3 mRNA及caspase-8 mRNA的表达情况。
结果与结论：在增生性瘢痕组织中，caspase-3 mRNA及caspase-8 mRNA表达的相对值分别为8.31±1.47和7.12±1.24，均明显低于正常皮肤组织(P < 0.05)。提示caspase-3/8在瘢痕形成过程中起着得要的作用，增生性瘢痕的形成可能与组织中caspase-3/8表达低下有关。

关键词: 病理性瘢痕, 增殖期, 半胱氨酸天冬氨酸蛋白酶, 基因表达, 凋亡

Abstract:

BACKGROUND: Apoptosis participates in the dynamic process of cell component decease due to granulation tissues transforming into scar tissues, and Caspase family mediated apoptosis is the main pathway for apoptosis. Severe burns often reduces the formation of hypertrophic scars, Seriously affected in patients with body function and aesthetic, the treatment of hypertrophic scars are still exploring at the molecular level.
OBJECTIVE: To explore the role of caspase-3 mRNA and caspase-8 mRNA in hypertrophic scars at the gene level.
METHODS: Pathological scar tissue and normal skin tissue were obtained from 18 patients who received treatment at the Department of Burn and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University. Additional normal skin tissues served as controls. The expression of caspase-3 mRNA and caspase-8 mRNA in hypertrophic scars was tested by semi-quantitative reverse polymerase chain reaction method.
RESULTS AND CONCLUSION: The expression rate of caspase-3 mRNA and caspase-8mRNA in hypertrophic scar were (8.31±1.47) and (7.12±1.24), respectively, which were significantly smaller than those of the normal skins (P < 0.05). The results indicated that caspase-3 mRNA and caspase-8 mRNA might play an important role in the process of wound healing and that the formation of hypertrophic scar might be due to the down-regulation of caspase-3 mRNA or (and) caspase-8 mRNA expression.

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