中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4435-4438.doi: 10.3969/j.issn.1673-8225.2010.24.015

• 组织构建与生物力学 tissue construction and biomechanics • 上一篇    下一篇

失神经骨骼肌萎缩中泛素蛋白连接酶Murf1和核转录因子NF-κB表达与被动运动干预

韩利军，梁炳生，王  乐，张  磊   

  1. 山西医科大学第二医院骨科，山西省太原市 030001
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 通讯作者: 梁炳生，博士，主任医师，博士生导师，山西医科大学第二医院骨科，山西省太原市 030001 rjjhlj318755@163.com
  • 作者简介:韩利军★，男，1981年生，山西省太原市人，汉族，山西医科大学在读硕士，主要从事骨科学方面的研究。 hanlijun318755@tom.com

Expression of ubiquitin-protein ligase Murf1 and nuclear factor kappa B in denervated amyotrophy intervened by passive movement

Han Li-jun, Liang Bing-sheng, Wang Le, Zhang Lei   

  1. Department of Orthopaedics, the Second Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Liang Bing-sheng, Doctor, Chief physician, Doctoral supervisor, Department of Orthopaedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China rjjhlj318755@163.com
  • About author:Han Li-jun★, Studying for master’s degree, Department of Orthopaedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China hanlijun318755@tom.com

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摘要：

背景：蛋白质分解是延缓肌萎缩发生的关键环节，在慢性病性、制动性肌萎缩中泛素蛋白连接酶Murf1和核转录因子NF-κB的表达增加，被动运动已被证实可以有效抑制肌萎缩的发生。
目的：探讨泛素蛋白连接酶Murf1和核转录因子NF-κB在大鼠失神经肌萎缩中不同时段的表达，以及被动运动对失神经骨骼肌Murf1和NF-κB表达的影响。
方法：假手术组大鼠不切断右下肢坐骨神经，失神经组、失神经被动运动组大鼠切断右下肢坐骨神经。术后1 d起，将失神经被动运动组大鼠置于自制的网夹内，拉出右后肢，抓住趾部，与脊柱呈45°向后外方牵拉，至右后肢完全伸直，再将右后肢推向身体，使之完全屈曲紧贴身体，每天训练2次，每次屈伸运动300下，3 min/次，直至切取标本之日。干预2，14，28 d后，采用RT-PCR与Western Blot技术分别检测Murf1，NF-κB mRNA和蛋白质的表达。
结果与结论：与假手术组比较，各时间点失神经组Murf1，NF-κB mRNA及蛋白的表达均明显增加(P < 0.05)；与失神经组比较，各时间点失神经被动运动组Murf1及NF-κB mRNA的表达均显著降低(P < 0.01)。失神经支配后肌湿质量比明显下降，被动运动14 d时肌湿质量比明显高于失神经组(P < 0.05)。失神经腓肠肌中Murf1，NF-κB mRNA和蛋白的表达与肌湿质量呈负相关(r= -0.795，P < 0.01；r=-0.834，P < 0.01)，提示被动运动可能通过降低Murf1和NF-κB的表达发挥肌萎缩防治作用。

关键词: 失神经, 肌萎缩, 被动运动, Murf1, NF-&kappa, B, 肌肉肌腱组织工程

Abstract:

BACKGROUND: Protein degradation is a key link in delaying amyotrophy. The expressions of muscle ring finger protein (Murf1) and nuclear factor κB (NF-κB) are increased in amyotrophy, and studies have been demonstrated that passive movement can suppress the occurrence of amyotrophy.
OBJECTIVE: To investigate expression pattern of Murf1 and NF-κB during denervated amyotrophy. at different time points, and to explore effects of passive movement on the expression of Murf1 and NF-κB in denervated amyotrophy.
METHODS: Sciatic nerves of rats were cut down in the denervated and passive movement groups. Sham operation was performed in the sham-operated group. Rats in the passive movement group were performed passive movement (45° traction and full range flexion) at 1 day after operation, twice per day, 300 frequency per time. The expressions of Murf1 and NF-κB were determined by RT-PCR and Western blot at 2, 14 and 28 days after intervention.
RESULTS AND CONCLUSION: Compared with the sham-operated group, the expressions of Murf1, NF-κB mRNA and protein in the denervated group were obviously increased at each time points (P < 0.05). Compared with the denervation group, the expressions of Murf1, NF-κB mRNA in the passive movement group were dramatically decreased (P < 0.01). The muscle wet weight was obviously decreased after denervation, which was greater in the positive movement group than that of the denervation group at 14 days (P < 0.05). The expression of mRNA and protein of Murf1 and NF-κB were negatively correlated to muscle wet weight in the denervated group (r = -0.795, P < 0.01; r = -0.834, P < 0.01). Passive movement can prevent amyotrophy by reducing the expressions of Murf1 and NF-κB.

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