中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2696-2700.doi: 10.3969/j.issn.1673-8225.2010.15.009

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

兔前交叉韧带切断后外侧半月板退变中基质金属蛋白酶13的作用

李国军1，李康华2，张世清1，王  晓1   

  1. 1河南大学淮河医院骨科，河南省开封市  475001；  2中南大学湘雅医院骨科，湖南省长沙市  410008
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 作者简介:李国军☆，男，1971年生，河南省尉氏县人，汉族，2009年中南大学毕业，博士，主治医师，主要从事骨与关节损伤的研究。 lgjhndx@yahoo.com.cn

Role of matrix metalloproteinase-13 in the degenerated lateral meniscus of rabbits with anterior cruciate ligament rupture 

Li Guo-jun1, Li Kang-hua2, Zhang Shi-qing1, Wang Xiao1   

  1. 1 Department of Orthopaedics, Huaihe Hospital of Henan University, Kaifeng  475001, Henan Province, China; 2 Department of Orthopaedics, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • About author:Li Guo-jun☆, Doctor, Attending physician, Department of Orthopaedics, Huaihe Hospital of Henan University, Kaifeng 475001, Henan Province, China lgjhndx@yahoo.com.cn

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323

被引次数

2

摘要：

背景：研究证明，膝关节前交叉韧带切断后，基质金属蛋白酶13在关节软骨细胞外基质降解中起重要作用，然而基质金属蛋白酶13在外侧半月板退变中有何作用，作者尚未查及到文献报道。
目的：探讨兔前交叉韧带切断后外侧半月板中基质金属蛋白酶13阳性表达率的变化和意义。
方法：48只新西兰兔膝关节配对为实验侧和对照侧造模，造模后第1，3，6，8周各随机处死12只，通过大体观察和苏木精-伊红染色对外侧半月板退变进行评分，免疫组织化学检测基质金属蛋白酶13表达。
结果与结论：随时间延长，与对照组比较，实验组外侧半月板退变评分逐渐增高(P < 0.05)。各时间点实验组基质金属蛋白酶13阳性表达率均较对照组增高(P < 0.05)。实验组基质金属蛋白酶13阳性表达率在3，6周显著高于1，8周(P < 0.05)，8周显著高于1周(P < 0.05)，3，6周差异无显著性意义(P > 0.05)。结果提示基质金属蛋白酶13参与了外侧半月板组织的退变；基质金属蛋白酶13阳性表达率降低并不表示外侧半月板组织退变结束。

关键词: 前交叉韧带, 半月板, 基质金属蛋白酶13, 组织构建, 软骨组织工程

Abstract:

BACKGROUND: Previous studies demonstrated that matrix metalloproteinase-13 (MMP-13) plays a great role in anterior cruciate ligament (ACL) rupture-induced degradation of hyaline cartilage extracellular matrix of knee joint, however, the role of MMP-13 in the degeneration of lateral meniscus is poorly understood.
OBJECTIVE: To investigate the changes and significance of the MMP-13 expression in the lateral meniscus of rabbit with ACL rupture.
METHODS: Lateral meniscus of 48 rabbits was randomly divided into experimental side and control side, and 12 rabbits were randomly selected and sacrificed at 1, 3, 6 and 8 weeks after model preparation. The degeneration of lateral meniscus was scored through gross and haematoxylin-eosin staining, and the expression of MMP-13 was detected using immunohistochemistry.
RESULTS AND CONCLUSION: With time prolonged, the scores of lateral meniscus degeneration were increased in the experimental group than that of the control group (P < 0.05). Compared with the control group, the positive expression of MMP13 was greater in the experimental group at each time point (P < 0.05). In the experimental group, positive expression of MMP13 was greater at 3 and 6 weeks than that of the 1 and 8 weeks (P < 0.05), which was significantly higher at 8 weeks than 1 week (P < 0.05), however, there was no significant difference between 3 and 6 weeks (P > 0.05). MMP-13 maybe a promotional factor which causes the degeneration of lateral meniscus; the decreasing of the MMP-13 expression is not a stopping signs of lateral meniscus degeneration.

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