中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2631-2634.doi: 10.3969/j.issn.1673-8225.2010.14.034

• 干细胞综述 stem cell review • 上一篇    下一篇

神经干细胞在脊髓损伤中的应用与进展

陈嘉利1，周继辉1，郎金波2   

  1. 1大庆油田总院集团龙南医院即齐齐哈尔医学院附第五医院骨科，黑龙江省大庆市163453；2大庆油田总院集团过敏病医院，黑龙江省大庆市  163000
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 周继辉，博士，大庆油田总院集团龙南医院即齐齐哈尔医学院附第五医院骨科，黑龙江省大庆市 163453 zhoujihui321@163.com
  • 作者简介:陈嘉利，男，1975年生，黑龙江省大庆市人，汉族，大庆医学高等专科学校毕业，主治医师，主要从事骨科研究。

Application and progress of neural stem cells in spinal cord injury

Chen Jia-li1, Zhou Ji-hui1, Lang Jin-bo2   

  1. 1 Department of Orthopaedics, Longnan Hospital of Daqing Oilfield General Hospital Group, Fifth Affiliated Hospital of Qiqihar Medical College, Daqing  163000, Heilongjiang Province, China; 2 Daqing Allergic Disease Hospital of Daqing Oilfield General Hospital Group, Daqing  163000, Heilongjiang Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Zhou Ji-hui, Doctor, Department of Orthopaedics, Longnan Hospital of Daqing Oilfield General Hospital Group, Fifth Affiliated Hospital of Qiqihar Medical College, Daqing 163000, Heilongjiang Province, China zhoujihui321@163.com
  • About author:Chen Jia-li, Attending physician, Department of Orthopaedics, Longnan Hospital of Daqing Oilfield General Hospital Group, Fifth Affiliated Hospital of Qiqihar Medical College, Daqing 163000, Heilongjiang Province, China

PDF

388

被引次数

6

摘要：

背景：神经干细胞来源广泛，取材方便，分离培养容易，且神经干细胞易于外源基因的导入和表达，可为基因治疗神经系统的疾病提供良好的载体。
目的：对神经干细胞在脊髓损伤中的应用进行回顾性研究。
方法：由通讯作者检索2006/2009 PubMed数据(http://www.ncbi.nlm.nih.gov/PubMed)及万方数据库(http://www. wanfangdata.com.cn)有关神经干细胞的培养，鉴定，分化，及其应用于脊髓损伤的组织工程修复等方面的文献，英文检索词为“neural stem cells，spinal cord injury ，cellular transplantation”，中文检索词为“神经干细胞，脊髓损伤，细胞移植”。排除重复性研究。计算机初检得到82篇文献，根据纳入标准保留23篇进一步归纳总结。
结果与结论：神经干细胞移植的动物实验已广泛开展。目前神经干细胞在修复脊髓损伤中的应用主要集中以下几方面：一是直接细胞移植进行替代治疗，通过神经干细胞的移植或激活体内的神经干细胞，使其分化为神经元和胶质细胞，并与已经存在的神经细胞结构整合到一起而达到治疗疾病的目的；二是以神经干细胞作为基因载体，携带治疗作用的目的基因进行移植，从而达到细胞替代和基因治疗的双重作用；三是通过对生长因子和细胞因子的研究，诱导自身的神经干细胞分化进行神经自我修复。

关键词: 神经干细胞, 脊髓损伤, 细胞移植, 干细胞, 移植, 综述文献

Abstract:

BACKGROUND: Neural stem cells (NSCs) are characterized by widely resources, convenient harvesting, easy culture and prone to import and express exogenous genes, which can be served as carriers of gene therapy for neural system disease.
OBJECTIVE: To review the application of NSCs in spinal cord injury.
METHODS: Databases of PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang (http://www.wanfangdata.com.cn) were searched by the correspondence author using key words of “neural stem cells, spinal cord injury, cellular transplantation” both in English and Chinese to retrieve papers concerning isolation, identification, differentiation of NSCs as well as its application in repairing spinal cord injury. A total of 82 documents were initial obtained by computer, after repetitive studies were excluded, 23 papers were included in the final analysis. 
RESULTS AND CONCLUSION: The NSCs transplantation has been widely used in animal experiments. Currently, the applications of NSCs in repairing spinal cord injury are concentrated on the following aspects: Firstly, cellular replacement therapy, namely, direct transplanting NSCs or activating in vivo NSCs to differentiate into neurons and glial cells, and then integrating transplanted cells with the existed neural cellular structure to cure the disease. Secondly, NSCs were utilized as gene carriers, which carrying target gene to body and reach the aims of cellular replacement and gene therapy. Thirdly, autologous NSCs were induced differentiation for self neural repair via studying growth factors and cytokines.

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