中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2623-2626.doi: 10.3969/j.issn.1673-8225.2010.14.032

• 干细胞综述 stem cell review • 上一篇    下一篇

骨髓间充质干细胞定向分化研究中蛋白质组学的地位与应用

胡  峰，赵劲民   

  1. 广西医科大学第一附属医院创伤手外科，广西壮族自治区南宁市 530021
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 赵劲民，教授，主任医师，广西医科大学第一附属医院创伤手外科，广西壮族自治区南宁市 530021 zhaojinmin@126.com
  • 作者简介:胡 峰☆，男，1980年生，广西壮族自治区南宁市人，壮族，广西医科大学在读博士，主要从事骨科蛋白质组学方面的研究。 hfarena@126.com

Position and application of proteomics in induced differentiation of bone mesenchymal stem cells

Hu Feng, Zhao Jin-min   

  1. Department of Trauma and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Zhao Jin-min, Professor, Chief physician, Department of Trauma and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Hu Feng☆, Studying for doctorate, Department of Trauma and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China hfarena@126.com

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被引次数

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摘要：

背景：细胞分化的实质是细胞内基因的选择性表达，导致细胞新的特异性的蛋白质的合成，从而在生化、结构及功能上发生变化，原有的基因组和分析单一蛋白的模式已不能满足研究的需要。蛋白质组学技术是大规模、系统性研究蛋白质变化及其相互作用的强有力工具，可以用于骨髓间充质干细胞定向分化的分子机制研究当中。
目的：介绍蛋白质组学的概况，总结蛋白质组学在骨髓间充质干细胞向心肌、成骨及脂肪细胞定向分化方面的研究进展，讨论其面临的问题，并对蛋白质组学研究方法进行展望。
方法：在CNKI数字图书馆全文数据库、Sciencedirect 数据库、I.S.I 数据库中检索有关骨髓间充质干细胞特性、定向分化及蛋白质组学在骨髓间充质干细胞定向分化研究中应用的文章，纳入近年来起重大影响以及开启和引领新方向的重要研究成果。
结果与结论：纳入29篇文章进行综述，总结蛋白质组学技术在骨髓间充质干细胞向心肌、成骨及脂肪细胞分化领域提供的信息及积累的经验。随着蛋白质组学在方法学及技术上的不断创新和发展，将会成为研究骨髓间充质干细胞定向分化潜在机制的强有力工具，帮助人们早日揭开其中蕴含的生命奥秘。

关键词: 蛋白质组学, 干细胞, 骨髓, 间充质干细胞, 分化, 综述文献

Abstract:

BACKGROUND: The essence of cell differentiation is a selectively intra-cellular gene expression, which results in specific proteinic synthesis and causes changes in biochemistry, structure and function. Thus, original proteomics and a single protein analysis can not meet the requirement in study. Proteomics technology provides a powerful tool due to the large scale, systemical study of protein transformation and interaction, which can be used for exploring molecular mechanism of bone mesenchymal stem cells (BMSCs) during directional differentiation.
OBJECTIVE: To introduce proteomics, to summarize the research of proteomics in directional differentiation of BMSCs, and to forecast the development of proteomics research methods.
METHODS: To search articles highly related with BMSCs, cell differentiation, and proteomics published on CNKI (www.cnki.net/index.htm), Sciencedirect (http://www.Sciencedirect.com), I.S.I (http://www.isiwebofknowledge.com) were searched, and the key achievements were included in the analysis.
RESULTS AND CONCLUSION: A total of 29 documents were reviewed, and the experiences in the application of proteomics technology in the directional differentiation of BMSCs were summarized. With the innovation and development in methodology and technology, proteomics will become a powerful tool for us to study the potential mechanisms of BMSCs directional differentiation.

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