中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (14): 2533-2538.doi: 10.3969/j.issn.1673-8225.2010.14.013

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

蛛网膜下腔移植自体激活许旺细胞治疗大鼠脊髓损伤

王春源1，冯世庆1，刘  洋1，韩明远1，李  辉1，陈家童2   

  1. 1天津医科大学总医院骨科，天津市 300052；2 南开大学生命科学院公共细胞培养室，天津市  300071
  • 出版日期:2010-04-02 发布日期:2010-04-02
  • 通讯作者: 冯世庆，硕士，主任医师，博士生导师，天津医科大学总医院骨科，天津市 300052 fengsq321@sina.com
  • 作者简介:王春源★，男，1983年生，河北省蔚县人，汉族，2010年天津医科大学毕业，硕士，主要从事脊柱脊髓损伤方面的研究。 a1984081519830326@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(30872603)，课题名称“磁靶向介导C3联合自体激活雪旺细胞和骨髓间充质干细胞治疗脊髓损伤的实验研究”；教育部新世纪人才支持计划(NCET-06-0251)，课题名称“RhoA抑制剂C3联合自体激活SC和HUCBCs修复脊髓损伤的研究”；天津市科委基金(07JCYBJC10200)，课题名称“RhoA抑制剂C3联合自体激活雪旺细胞修复脊髓损伤的研究”。

Autologous activated Schwann cells transplantation via subarachnoid space for rat spinal cord injury 

Wang Chun-yuan1, Feng Shi-qing1, Liu Yang1, Han Ming-yuan1, Li Hui1, Chen Jia-tong2   

  1. 1 Department of Orthopaedics, General Hospital of Tianjin Medical University, Tianjin   300052, China; 2 Public Cell Culture Laboratory, College of Life Sciences, Nankai University, Tianjin   300071, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Feng Shi-qing, Master, Chief physician, Doctoral supervisor, Department of Orthopaedics, General Hospital of Tianjin Medical University, Tianjin 300052, China fengsq321@sina.com
  • About author:Wang Chun-yuan★, Master, Department of Orthopaedics, General Hospital of Tianjin Medical University, Tianjin 300052, China a1984081519830326@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No.30872603*; the New Century Excellent Talents in University, the Ministry of Education, No.NCET-06-0251*; a Grant from Tianjin Science and Technology Commission, No. 07JCYBJC10200*

PDF

380

被引次数

3

摘要：

背景：许旺细胞能够分泌多种神经营养因子，促进脊髓损伤功能的恢复。但异体许旺细胞移植可引发自身免疫反应，且在移植方式上，局部移植无法避免二次损伤，静脉移植虽可以透过血脊髓屏障到达损伤局部，但不能达到有效的治疗浓度。
目的：探讨经蛛网膜下腔移植自体激活许旺细胞对脊髓损伤大鼠功能恢复的影响。
方法：66只大鼠均建立脊髓损伤模型，造模后随机分为3组，自体激活许旺细胞组通过结扎单侧隐神经从而激活许旺细胞，自体未激活许旺细胞组、模型对照组仅在相同部位手术但不结扎神经。切除各组手术远端1 cm神经，采用组织块法进行许旺细胞的体外分离培养及纯化。1周后，自体激活许旺细胞组、自体未激活许旺细胞组分别通过蛛网膜下腔注入经Hoechst33342标记的对应许旺细胞悬液，模型对照组仅注入等量DMEM。对脊髓损伤后肢体功能的恢复进行BBB运动功能评分及脚印分析，通过苏木精-伊红染色和GFAP染色从组织学角度评价脊髓损伤恢复情况。
结果与结论：从术后第4周开始，自体激活许旺细胞组BBB后肢功能评分明显优于另两组(P < 0.05)。移植后2周，可见迁移至大鼠脊髓损伤局部的许旺细胞。与自体未激活许旺细胞组比较，移植后5周自体激活许旺细胞组的前后足中心距离、后肢第3足趾外旋角度均显著减小(P < 0.05)，移植后13周损伤区胶质瘢痕面积明显减小(P < 0.05)，损伤区空洞面积明显减小(P < 0.05)。证实经蛛网膜下腔移植自体激活许旺细胞可以促进脊髓损伤的恢复。

关键词: 脊髓损伤, 后肢功能, 恢复, 蛛网膜下腔, 移植, 自体激活许旺细胞

Abstract:

BACKGROUND: Schwann cells can secrete various neurotrophic factors, and promote functional recovery of injured spinal cord. However, xenogenic Schwann cells transplantation may induce autoimmune response. Moreover, local transplantation results in secondary injury. Vein transplantation may reached injury site passing the blood spinal cord barrier, but the treatment concentration is not effective.
OBJECTIVE: To investigate the therapeutic effects of transplantation of autologous activated Schwann Cells (AASCs) via subarachnoid space on spinal cord injury (SCI) in rats.
METHODS: A total of 66 rats were used to establish SCI models, and the model rats were randomly divided into 3 groups. The unilateral saphenous nerves of rats were ligated directly for 1 week to activate Schwann cells, but inactivated and model control groups were not subjected to nerve ligation. 1 cm nerve was excised from distal end of each group, and Schwann cells were isolated and cultured by tissue mass method. The AASCs, autologous Schwann cells (ASCs) were injected with corresponding Hoechst33342-labeled SCs suspension, but the model control group was injected with DMEM injection. The basso beattie bresnahan (BBB) score and footprint analysis, as well as HE and GFAP immunohistochemistry staining were performed to evaluate functional recovery of rat hind limbs.
RESULTS AND CONCLUSION: On 4 weeks after injury, BBB scores of AASCs were significantly superior to the other groups (P < 0.05). Two weeks after transplantation, some SCs migrated to injured spinal cord. Compared with ASCs group, the center distance of forward and hind feet and extorsion angle of the third toe of hind limb were significantly reduced in the AASCs group at 5 weeks (P < 0.05), the glial scar area was significantly decreased at 13 weeks (P < 0.05), and the cavity area of injured region was significantly diminished (P < 0.05). Results show that AASCs transplantation via subarachnoid space promoted functional recovery after SCI in rats.

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