中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (12): 2153-2158.doi: 10.3969/j.issn.1673-8225.2010.12.016

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

钛合金表面纳米结构对钙磷矿化物体外沉积及成骨细胞矿化功能的影响

张吉宏，赵常利，张小农   

  1. 上海交通大学材料科学与工程学院金属基复合材料国家重点实验室，上海市  200240　
  • 出版日期:2010-03-19 发布日期:2010-03-19
  • 通讯作者: 张小农，副教授，上海交通大学材料科学与工程学院复合材料国家重点实验室，上海 200240 xnzhang@sjtu.edu.cn
  • 作者简介:张吉宏★，男，1983年生，辽宁省普兰店市人，汉族，2010年上海交通大学毕业，硕士，主要从事金属生物材料研究。 henryzjh@gmail.com
  • 基金资助:

    上海市科委纳米专项基金（0852nm02800）。

Effect of nanostructure of nano-titanium alloy on in vitro calcium and phosphorus minerals deposition and osteoblast mineralization

Zhang Ji-hong, Zhao Chang-li, Zhang Xiao-nong   

  1. State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai  200240, China　
  • Online:2010-03-19 Published:2010-03-19
  • Contact: Zhang Xiao-nong, Associate Professor, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China xnzhang@sjtu.edu.cn
  • About author:Zhang Ji-hong★, Master, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China henryzjh@gmail.com
  • Supported by:

    the Nano Foundation of Science and Technology Commission of Shanghai Municipality, No. 0852nm02800*

PDF

568

被引次数

15

摘要：

背景：通过改变金属材料置入假体表面物理性质，可以增强细胞的成骨性能，表面纳米化处理是当前研究的一个重要方向。
目的：通过测定纳米钛合金和未处理微米钛合金表面钙磷沉积量及成骨细胞在两种材料表面的钙化功能，评估材料表面纳米结构对钛合金生物相容性的影响。
方法：采用表面机械研磨处理(SMAT)制备了表面纳米结构的Ti6Al4V钛合金，将钛合金表面的微米级晶粒转变为纳米尺寸的晶粒。实验分为微米合金和纳米合金组，将试样浸入模拟体液中浸泡21 d。分离新生乳鼠颅盖骨成骨细胞进行培养，将其分别接种到纳米表面和微米表面钛合金上进行共培养14 d。观察两组合金表面粗糙度、润湿角、材料表面钙磷沉积量、成骨细胞钙结节形成的情况。
结果与结论： ①基于SMAT技术的表面纳米钛合金表面得到粗糙表面，其组成的晶粒则为纳米量级，表面粗糙度(Ra)由  132.5 nm增加到4019.3 nm；接触角由57.26°减小到22.4°；表面能由39.4 mJ/m2增加到67.3 mJ/m2。②在无成骨细胞的模拟体液中沉积7，14，21 d之后，纳米Ti6Al4V合金表面的钙磷元素沉积量显著增加。③纳米钛合金表面形成的钙结节面积显著大于微米钛合金表面，培养14 d后前者约为后者的3倍。结果提示，表面纳米钛合金能明显促进成骨细胞的矿化，并增加表面钙磷的沉积，具有较好的生物相容性。

关键词: 纳米结构, Ti6Al4V, 成骨细胞, 矿化, 钙磷沉积, 纳米生物材料

Abstract:

BACKGROUND: Surface nanostructure is an important direction for enhancing osteogenesis. 
OBJECTIVE: To evaluate the effect of surface nanostructure on the biocompatibility of titanium by measuring the calcium and phosphorus minerals depositions and osteoblast mineralization on nanostructure and untreated microstructure titanium.
METHODS: The surface mechanical attrition treatment (SMAT) technique was used to prepare nano-crystalline surface on Ti6Al4V alloy. After treatment, the microsized grains on the titanium substrates were refined to nanosize. Then the SMAT nanostructure and untreated microstructure titanium substrates were immersed in the simulated body fluid for 21 days. The osteoblasts isolated from the neonatal rat calvarias were incubated and cultured on the surface of nanophase and microphase titanium alloy for 14 days culture. The surface roughness, contact angle, the difference of calcium and phosphorus minerals formation, numbers and area of calcium nodules were observed.
RESULTS AND CONCLUSION:  ① Compared to the microphase titanium alloy, the surface roughness (Ra) of nanophase titanium alloy was increased from 132.5 nm to 4 019.3 nm, and the contact angle decreased from 57.26° to 22.4° and surface energy increased from 39.4 mJ/m2 to 67.3 mJ/m2. ② The results of the deposition experiments provided the evidence of greater calcium and phosphorus precipitations from culture media on nanophase compared to microphase Ti6Al4V at 7, 14 and 21 days. ③ On the nanophase alloy, the presence of strong cellular mineralization could be observed. Calcium nodules formed on the surface of nano-titanium alloy were significantly greater than normal surface alloy and the area of the former was about 3 times larger than the latter after 14 days. The nano-surface titanium alloy significantly promoted the extracellular mineralization of osteoblasts and increased the precipitations of calcium and phosphorus minerals, which showed good biocompatibility.

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