中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1377-1381.doi: 10.3969/j.issn.1673-8225.2010.08.012

• 药物控释材料 drug delivery materials • 上一篇    下一篇

应用三丙烯微球对豚鼠子宫肌瘤模型行子宫动脉栓塞的实验

谭国胜，杨建勇，郭文波，向贤宏，范惠双，侯昌龙   

  1. 中山大学附属第一医院介入放射科，广东省广州市 510080
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 通讯作者: 郭文波，博士，副主任医师，副教授，中山大学附属第一医院介入放射科，广东省广州市 510080
  • 作者简介:谭国胜，男，1976年生，广东省新会市人，汉族，2000年中山医科大学毕业，主治医师，主要从事介入放射诊断与治疗研究。 tgs1976@163.com
  • 基金资助:

    广东省自然科学基金博士启动项目(07300941)。

Effect of trisacryl gelatin microspheres on uterine arterial embolization of a hysteromyoma guinea pig model

Tan Guo-sheng, Yang Jian-yong, Guo Wen-bo, Xiang Xian-hong, Fan Hui-shuang, Hou Chang-long   

  1. Department of Interventional Radiology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Online:2010-02-19 Published:2010-02-19
  • Contact: Guo Wen-bo, Doctor, Associate chief physician, Associate professor, Department of Interventional Radiology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China kerisgz@126.com
  • About author:Tan Guo-sheng, Attending physician, Department of Interventional Radiology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China tgs1976@163.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 07300941*

PDF

512

被引次数

8

摘要：

背景：子宫动脉栓塞术作为治疗子宫肌瘤的重要手段已广泛应用于临床，疗效令人满意，但对子宫动脉栓塞术的基础研究目前尚处于起步阶段，理论系统尚不完善。应用新型栓塞剂三丙烯微球行豚鼠子宫肌瘤模型进行子宫动脉栓塞操作的相关文献报道作者尚未查到。
目的：实验设计了以三丙烯微球进行豚鼠子宫肌瘤模型子宫动脉栓塞的可行性验证。
方法：30只成年雌性豚鼠随机分为两组：盆腔动脉铸型组10只，用于行盆腔血管铸型术，了解雌性豚鼠子宫动脉起源、行程、长度、直径及分支等解剖学特征；子宫肌瘤模型动脉栓塞组20只，应用雌孕激素联合法诱导子宫肌瘤模型建立，并行双侧子宫动脉微球栓塞的技术研究及病理分析。
结果与结论：双侧子宫动脉主干由髂内动脉发出，主干和弓状支的血管直径分别为(0.350±0.022) mm及(0.160±0.012) mm。子宫肌瘤模型动脉栓塞组20只豚鼠成功行双侧子宫动脉栓塞术。术中40~120 µm及100~300 µm三丙烯微球的用量分别为(0.040±0.005) mL和(0.017±0.002) mL。子宫肌瘤模型动脉栓塞组肌瘤成模率达75%。病理切片可见子宫动脉弓状支、浆膜下二级分支及部分三级分支动脉内均有栓塞微球存在。子宫肌层增厚，肌瘤结节处细胞呈栅栏状或编织状排列。栓塞后肌瘤缺血坏死明显，子宫肌层及内膜无缺血坏死病理改变。说明应用三丙烯微球行豚鼠子宫肌瘤模型动脉栓塞术具有可行性，栓塞效果良好。

关键词: 三丙烯微球, 子宫动脉栓塞术, 豚鼠, 子宫肌瘤, 控制释放载体材料

Abstract:

BACKGROUND: The uterine arterial embolization which is a major method to treat hysteromyoma has been widely used in clinic and achieved a satisfactory therapeutic efficacy. The study addressing the effect of trisacryl gelatin microspheres on uterine arterial embolization in a hysteromyoma guinea pig model has less been reported yet.
OBJECTIVE: To verify the feasibility of trisacryl gelatin microspheres to uterine arterial embolization in hysteromyoma guinea pig models.
METHODS: A total of 30 adult female guinea pigs were randomly divided into two groups: pelvic cavity artery moulding group (n=10) was performed pelvic vascular casting mould to demonstrate the anatomical characteristics, such as source, running shape, length, diameter and branches; arterial embolization group (n=20) was induced hysteromyoma model using estrogen-progestogen replacement therapy and performed technical research and pathological analysis by bilateral uterine arterial embolization.
RESULTS AND CONCLUSION: The trunks of uterine arteries were erupted from internal iliac arteries. The diameter of the trunks and its arcuate branches were (0.350±0.022) mm and (0.160±0.012) mm, respectively. The 20 guinea pigs of the arterial embolization group were succeeded in operating bilateral arterial embolization. The dosage of 40-120 µm and 100-300 µm trisacryl gelatin microspheres were (0.040±0.005) mL and (0.017±0.002) mL respectively during the operation. The achievement ratio of establishing model was 75% in the arterial embolization group. On the pathological section, the microspheres could be found in the uterine arterial arcuate branches and second branches within the subserosa and third branches. The myometrium was thickening. The cells of the leiomyoma nodules arranged in palisade or weaving shapes. Ischemia and necrosis were evidently present in leiomyomas of guinea pigs after embolization, but the myometria and endometria had no pathological change of ischemia and necrosis. It is feasible to use trisacryl gelatin microspheres to operate uterine arterial embolization for hysteromyoma of guinea pigs and the embolization effects are satisfactory.

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