中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1341-1344.doi: 10.3969/j.issn.1673-8225.2010.08.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

多孔型丝素蛋白/羟基磷灰石复合脂肪间充质干细胞修复兔股骨骨缺损

施咏毅1，王根林1，杨惠林1，卢神州2，张  亚3，蔡  鑫1   

  1. 1苏州大学附属第一医院骨科，江苏省苏州市 215006；2苏州大学材料工程学院，江苏省苏州市   215006；3苏州大学附属儿童医院，江苏省苏州市  215003
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 通讯作者: 杨惠林，苏州大学附属第一医院骨科，江苏省苏州市 215006 wglpaper@126.com
  • 作者简介:施咏毅★，男，1975年生，江苏省苏州市人，汉族，苏州大学在读硕士，主治医师，主要从事脊柱外科、创伤骨科和人工骨研究。 Charleyshi2406@sina.com
  • 基金资助:

    江苏省基础研究计划(自然科学基金)项目(BK2008008)。

Repairing rabbit femur bone defects by porous silk fibroin/hydroxyapatite combined with adipose-derived stromal cells

Shi Yong-yi1, Wang Gen-lin1, Yang Hui-lin1, Lu Shen-zhou2, Zhang Ya3, Cai Xin1   

  1. 1 Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou  215006, Jiangsu Province, China; 2 Institute of Material & Engineering, Soochow University, Suzhou  215006, Jiangsu Province, China; 3 Soochow University Affiliated Children’s Hospital, Suzhou  215006, Jiangsu Province, China.
  • Online:2010-02-19 Published:2010-02-19
  • Contact: Wang Gen-lin, Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China wglpapwer@126.com
  • About author:Shi Yong-yi★, Studying for master’s degree, Attending physician, Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China Charleyshi2406@sina.com
  • Supported by:

    Basic Research Programs (Natural Science Foundation) of Jiangsu Province, No. BK2008008*

PDF

410

被引次数

27

摘要：

背景：从蚕丝中提取的丝素蛋白具有良好的细胞相容性，且可生物降解，用于修饰生物材料能提高细胞在材料表面的黏附和生长能力。
目的：探讨丝素蛋白/羟基磷灰石材料复合脂肪间充质干细胞修复包含性骨缺损的作用。
方法：取2月龄新西兰大白兔附睾处脂肪组织，经胰酶消化传代培养脂肪间充质干细胞，取第3代兔脂肪间充质干细胞以1×1010 L-1浓度接种于丝素蛋白/羟基磷灰支架材料上，培养3 h后加入含有1 μmol/L地塞米松、50 μmol/L维生素 C10 mmol/L β-甘油磷酸钠的DMEM培养液进行成骨诱导。新西兰大白兔36只，在兔股骨远端制备直径4.5 mm、深10 mm的松质骨缺损。细胞复合材料组植入复合脂肪间充质干细胞的丝素蛋白/羟基磷灰石；单纯材料组植入丝素蛋白/羟基磷灰石；空白对照组不作任何植入。
结果与结论：12周时大体观察细胞复合材料组骨缺损区完全被骨组织修复，单纯材料组骨缺损区缩小、部分修复，空白对照组骨缺损无修复。12周时X射线、组织学检查显示细胞复合材料组完全修复了骨缺损区，材料组部分修复了骨缺损区，细胞复合材料组的新生骨小梁多于单纯材料组(P < 0.05)，空白对照组未见骨修复。结果说明复合脂肪间充质干细胞的丝素蛋白/羟基磷灰石修复兔股骨包含性骨缺损能力优于单纯丝素蛋白/羟基磷灰石材料。

关键词: 丝素蛋白/羟基磷灰石, 脂肪间充质干细胞, 骨缺损, 骨及软骨组织工程支架材料, 复合生物材料

Abstract:

BACKGROUND: Silk fibroin derived from silk had a good biocompatibility and biodegradation, which could be used for biomaterials to improve cell adhesion and growth abilities.
OBJECTIVE: To investigate the efficacy of silk fibroin/hydroxyapatite (SF/HA) compounded of adipose-derived stromal cells (ADSCs) on repairing bone defects.
METHODS: Adipose tissues were derived from epididymis of 2-month-old New Zealand rabbits and trypsogen-passaged to obtain ADSCs. The third-passage ADSCs at the concentration of 1×10/ L were placed on SF/HA scaffold. Three hours later, the composite was cultured with DMEM culture media containing 1 μmol/L dexamethasone, 50 μmol/L vitamin C, and 10 mmol/L β-sodium glycerophosphate. Thirty-six rabbits were induced cancellated bone defect sizing 4.5 mm × 4.5 mm × 10 mm. The composite group was implanted with SF/HA/ADSCs scaffold, the simple group was implanted with SF/HA scaffold, but any treatment was employed in the blank control group.
RESULTS AND CONCLUSION: At 12 weeks, general observation demonstrated that the bone defects were repaired entirely in composite group and partly in simple group. However, the bone defect was not repaired in the blank control group. X-ray and histological observation suggested that at 12 weeks the bone defects were repaired entirely in composite group and partly in simple group. The quantity of the newly formed bone in the composite group was significantly more than that in the simple group (P < 0.05). Repair showed no effect in the blank control group. SF/HA/ADSCs composite could successfully repair bone defects of a rabbit femur, and the effect was superior to SF/HA scaffold.

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