中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (17): 3143-3147.doi: 10.3969/j.issn.1673-8225.201.17.026

• 骨与关节实验造模 experimental modeling of the bone and joint • 上一篇    下一篇

微波加热制作股骨头坏死模型：最适宜的温度和时间筛选

彭吾训1，王  蕾2，邓  进1，耿承奎3，李彦林3，龚跃昆3，李世和3   

  1. 1贵阳医学院附属医院急诊创伤外科，贵州省贵阳市 550004；2贵州省疾病预防控制中心职业病防治研究所，贵州省贵阳市  550004；3昆明医学院第一附属医院骨科，云南省昆明市 650031
  • 出版日期:2010-04-23 发布日期:2010-04-23
  • 通讯作者: 王 蕾，博士，主治医师，主要从事病理学方面的研究。贵州省疾病预防控制中心职业病防治研究所，贵州省贵阳市 550004，misswanglei@163.com
  • 作者简介:彭吾训☆，男，1971年生，云南省昆明市人，彝族，1993年昆明医学院毕业，博士，副教授，硕士研究生导师，主要从事骨创伤与骨组织工程方面的研究。 misswanglei@163.com
  • 基金资助:

    贵州省卫生厅基金项目(gzwkj2008-1-010)，课题名称：微波灭活建立兔股骨头坏死模型的实验研究。

Inducing a femoral head necrosis model by microwave heating: Screening of optimal temperature and time  

Peng Wu-xun1, Wang Lei2, Deng Jin1, Geng Cheng-kui3, Li Yan-lin3, Gong Yue-kun3, Li Shi-he3   

  1. 1 Department of Emergency and Trauma Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China; 2 Institute of Occupational Medicine, Guiyang Center for Disease Control and Prevention, Guiyang  550004, Guizhou Province, China; 3 Department of Orthopaedics, First Affiliated Hospital of Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Online:2010-04-23 Published:2010-04-23
  • Contact: Wang Lei, Doctor, Attending physician, Institute of Occupational Medicine, Guiyang Center for Disease Control and Prevention, Guiyang 550004, Guizhou Province, China misswanglei@163.com
  • About author:Peng Wu-xun☆, Doctor, Associate professor, Master’s supervisor, Department of Emergency and Trauma Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China misswanglei@163.com
  • Supported by:

     the Program of Health Department of Guizhou Province, No. gzwkj2008-1-010*

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摘要：

背景：目前股骨头坏死模型还不能很好地模拟临床股骨头坏死情况，利用微波灭活提供了一种新的方法，但是其具体的标准尚未确定。
目的：探讨微波灭活制作股骨头坏死模型的适宜温度和时间。
方法：将48只大白兔按数字随机法分为4组，将微波天线插入兔股骨头，4组分别以不同的时间(10，20 min)和温度(50，55，60 ℃)进行股骨头微波灭活，灭活后即刻、1，2，4，8和12周，观察兔行为、利用X射线、MRI检测股骨头影像，并观察股骨头切片的组织坏死及修复情况。 
结果与结论：50 ℃ 10 min条件微波灭活1周后，部分兔股骨头骨髓组织凝固变性，8周时坏死骨小梁及骨髓组织完全吸收。55 ℃ 10 min微波灭活1周后，兔股骨头骨髓组织凝固，2周时股骨头出现T1相信号减低、T2相信号增高区，4周时坏死与修复同时进行，12周时骨修复停止，骨坏死继续，股骨头开始塌陷。50 ℃ 20 min和60 ℃ 10 min微波灭活8周后，所有兔股骨头塌陷变形。由此认为，采用55 ℃ 10 min微波灭活股骨头是制作兔股骨头坏死模型的适宜温度和时间，其股骨头变化符合临床病理变化过程。

关键词: 股骨头坏死, 微波, 模型, 温度, 时间, 骨组织工程

Abstract:

BACKGROUND: The existed femoral head necrosis (FHN) model can not reflex the clinical necrosis of femoral head correctly. Microwave heating provides a new approach for model preparation, but the concrete standard remains uncertain.
OBJECTIVE: To study the optimal temperature and time in preparing a FHN model induced by microwave heating.
METHODS: Totally 48 rabbits were randomly divided into 4 groups on the basis of the microwave temperature (50, 55, 60℃) and heating time (10, 20 minutes). The microwave antenna was inserted into the rabbit femoral head. The rabbits were sacrificed immediately and at 1, 2, 4, 8 and 12 weeks after operation. A series of examinations were performed including gross observation, X-ray, histology and MRI to observe the femoral head necrosis and repair status.
RESULTS AND CONCLUSION: Marrow partially solidified in the group (50 ℃, 10 minutes) at 1 week, and the osteonecrosis returned to normal at 8 weeks after operation. In the group (55 ℃, 10 minutes), marrow was completely coagulated at 1 week and low signal on T1 weighted images and increased signal on T2 images were identified at 2 weeks. Osteonecrosis and repair occurred at the same time at 4 weeks. At 12 weeks, the osteonecrosis continued and the repair stopped, and the femoral heads started to collapse. All femoral heads collapsed at 8 weeks in group (50 ℃, 20 minutes) and group (60 ℃, 10 minutes). Accordingly, microwave heating is a good method in developing FHN model. 55 ℃ and 10 minutes are the optimal temperature and time for the development of FHN model of rabbits induced by microwave heating.

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