Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (27): 4269-4274.doi: 10.3969/j.issn.2095-4344.1371

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Bioinformatics analysis of gene spectrum of muscle atrophy after spinal cord injury 

Huang Hui, Wang Guangji   

  1.  (Department of Orthopedics, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China)
  • Received:2019-04-05 Online:2019-09-28 Published:2019-09-28
  • Contact: Wang Guangji, Chief physician, Department of Orthopedics, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China
  • About author:Huang Hui, Doctoral candidate, Department of Orthopedics, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China
  • Supported by:

    the Natural Science Foundation of Hainan Province, No. 310122 (to WGJ)

Abstract:

BACKGROUND: Muscle atrophy is often associated with spinal cord injury, but its underlying mechanisms are still unclear.
OBJECTIVE: To explore the molecular biological mechanism of muscle atrophy after spinal cord injury.
METHODS: Gene profile GSE45550 for muscle atrophy after spinal cord injury in the gene expression database was analyzed. The gene expression profile GSE45550 included a control group (pre-spinal cord injury), an experimental group 1 (3 days after spinal cord injury), an experimental group 2 (8 days after spinal cord injury), and an experimental group 3 (14 days after spinal cord injury). The tissue was the soleus muscle of Sprague-Dawley rats (n=6/group). Four groups of sample data were then subjected to differential gene analysis, GO analysis, and pathway analysis.
RESULTS AND CONCLUSION: Totally 2 513 differentially expressed genes were identified, of which Wnt16 Obfc1, Ufd1l, LOC100361067, Hhatl, Fxyd1, Psmc4, Tasp1, Mettl21c, and Ufd1l differential expressions were most significant. Biological processes such as biological process, G-protein coupled receptor signaling pathway, response to drug, transcription DNA-dependent, positive regulation of transcription DNA-dependent, oxidation-reduction process, ubiquitin-dependent protein catabolic process, apoptotic process, positive regulation of transcription from RNA polymeras, and fatty acid beta-oxidation, signaling pathways such as MAPK signaling, apoptosis, and citric acid cycle  may play important roles. This study completely reveals the differentially expressed genes of muscle atrophy after spinal cord injury gene profiles, the involved biological processes, and signaling pathways. Wnt16 may be a key gene in muscle atrophy after spinal cord injury, providing molecular targets for future therapeutic progress.

Key words: spinal cord injury, muscle atrophy, pathway, gene, biological process, differential gene analysis, GO analysis, pathway analysis

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