Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (3): 464-469.doi: 10.3969/j.issn.2095-4344.0598

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Resveratrol protects endothelial cells from oxidative damage and inhibits expression of pro-thrombosis molecules: the underlying mechanisms

He Wei, Lou Zhenkai, Wang Bing, Li Xingguo, Zhao Chongyu, Zhao Xueling   

  1.  (Department of Orthopedics, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China)
  • Supported by:

    the National Natural Science Foundation of China, No. 81160236 (to WB), 81760029 (to WB) and 81760030; the Science and Technology Program of Yunnan Province, No. 2016NS021, 2017NS021 and 2017NS022; the Doctoral Research Foundation of the First Affiliated Hospital of Kunming Medical University, No. 2017BS030

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Abstract:

BACKGROUND: Resveratrol has been shown to hold anti-oxidant and anti-arterial thrombosis effects.
OBJECTIVE: To explore the mechanism of resveratrol in repairing endothelial cell injury and inhibiting deep vein thrombosis at molecular level.
METHODS: Human umbilical vein endothelial cells were isolated and cultured. The cells were divided into three groups: control group (common medium), H2O2 group (cultured with 200 μmol/L H2O2 for 24 hours), and resveratrol plus resveratrol group (pretreated with 10, 20 and 30 μmol/L resveratrol for 2 hours, and then cultured with 200 μmol/L H2O2 for 24 hours). The cell viability was evaluated by MTT assay. The apoptotic cells were observed by Hoechst 33258 staining and fluorescence microscopy. The protein expression levels of P-selectin, P-selectin glycoprotein ligand-1, von Willebrand Factor and PI3-K/Akt/GSK3β and NF-κB signaling pathways were tested by western blot assay.
RESULTS AND CONCLUTION: Compared with the control group, H2O2 could induce endothelial cell injury and resveratrol could repair endothelial cells injured by H2O2 (P < 0.05). The apoptotic rate in the H2O2 group was significantly higher than that in the control group (P < 0.05). Compared with the H2O2 group, the apoptosis rate in the H2O2 plus resveratrol group was significantly decreased (P < 0.05). Compared with the control group, the expression levels of P-selectin, P-selectin glycoprotein ligand-1, von Willebrand Factor, NF-κB and GSK3β in the H2O2 group were significantly increased (P < 0.05), the expression of Akt protein showed no significant change (P > 0.05), and the expression level of p-Akt protein was significantly down-regulated (P < 0.05). Meanwhile, resveratrol could reverse all above protein expression (P < 0.05). To conclude, resveratrol can repair human umbilical vein endothelial cells and inhibit the expression of P-selectin, P-selectin glycoprotein ligand-1 and von Willebrand Factor proteins, which may be related to PI3-K/Akt/GSK3β and NF-κB signaling pathways.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Veratrum, Endothelial Cells, Oxidative Stress, Venous Thrombosis, Tissue Engineering

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