中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5797-5800.doi: 10.3969/j.issn.1673-8225.2011.31.023

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

新型免疫抑制剂J2对角膜移植小鼠淋巴细胞中白细胞介素10及干扰素γ分泌的影响

王大江1，郭惠玲1，黄一飞1，陈国江2，张  晗3，黎  燕2   

  1. 1解放军总医院眼科，全军眼科中心，北京市100853
    2解放军军事医学科学院基础医学研究所分子免疫室，北京市  100854
    3山东大学眼科中心，山东省济南市  250033
  • 收稿日期:2011-01-13 修回日期:2011-03-24 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 黄一飞，博士，博士导师，主任医师，教授，解放军总医院眼科，北京市 100853 3301yk@sina. com
  • 作者简介:王大江☆，男，1971年出生，辽宁省葫芦岛市人，汉族，2007年军医进修学院毕业，博士，副主任医师，主要从事角膜病及眼外伤研究。 wangdajiangv@ yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30973245)；全国博士后科学基金面上项目(20090451511)。

Effects of a new immunodepressant J2 on lymphocytic secretion of interleukin 10 and interferon gamma in mice after corneal allograft

Wang Da-jiang1, Guo Hui-ling1, Huang Yi-fei1, Chen Guo-jiang2, Zhang Han3, Li Yan2   

  1. 1Department of Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing  100853, China
    2Department of Molecular Immunology, Academy of Military Medical Sciences, Beijing  100854, China
    3Ophthalmology Center, Shandong University, Jinan  250033, Shandong Province, China
  • Received:2011-01-13 Revised:2011-03-24 Online:2011-07-30 Published:2011-07-30
  • Contact: Huang Yi-fei, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing 100853, China 3301yk@sina.com
  • About author:Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing 100853, China wangdajiangv@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30973245; the General Program of China Postdoctoral Science Foundation, No. 20090451511

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摘要：

背景：小分子化合物J2是以CD4为靶点的新型免疫抑制剂，课题组以往的实验已经验证了J2对正常小鼠及角膜移植后小鼠脾细胞的影响。
目的：探讨小分子化合物J2对异基因角膜小鼠的淋巴细胞中白细胞介素10及干扰素γ分泌的影响。
方法：BalB/c(H2d)小鼠随机数字表法分为4个组，安慰剂组、环孢素A 组和J2组接受C57BL/6-BALB/c同种异体角膜移植后给予相应药物干预，空白对照组不接受角膜移植，观察各组角膜移植片存活时间。取各组大鼠脾细胞给予刀豆蛋白Ⅵ型刺激细胞增生，采用ELISA法测定细胞上清中白细胞介素10及干扰素γ水平，比较各组细胞增生指数及细胞因子含量。
结果与结论：J2可能通过抑制CD4+T淋巴细胞而抑制角膜排斥反应的发生，延长角膜植片存活时间；J2能够明显抑制ConA刺激角膜移植后小鼠脾细胞的增生及Th1细胞因子的产生。这些效应与环孢素A的效果相似。

关键词: 角膜移植, 免疫抑制剂, 淋巴细胞, 环孢素A, 免疫排斥, 小鼠, 白细胞介素10, 干扰素&gamma

Abstract:

BACKGROUND: J2 is a new immunodepressant targeting CD4, and previous studies have verified the effect of J2 on spleen cells of normal mice or mice undergoing corneal transplantation.
OBJECTIVE: To investigate the effects of J2 on the lymphocytic secretion of interferon gamma (IFN-γ) and interleukin 10 (IL-10) of mice with allogeneic corneal transplantation.
METHODS: Corneal transplantation models were created in the right eyes of 36 clean BalB/c(H2d) mice (receiptors) and 17 clean C57BL/6(H2b) mice (donors). The models were randomly divided into four groups according to the different intervene methods. Corneal opacification and neovascularization were scored. The proliferation of spleen cells of mice was detected by ATPase method, and secretion of IL-10 and IFN- γ in cytosupernatant before and after ConA stimulated were assessed by ELISA. 
RESULTS AND CONCLUSION: J2 arrest the occurrence of corneal rejection by inhibiting the activation of CD4+ T cells and suppress the proliferation and ConA-stimulated Th1 cytokine production of spleen cells.

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