中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (32): 6031-6035.doi: 10.3969/j.issn.1673-8225.2010.32.031

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

不同来源干细胞神经向分化能力的研究现状

诸葛栋1，梁战华2，宋  琳1，刘  晶1   

  1. 大连医科大学附属第一医院，1中英再生医学应用研究中心，2神经内科，辽宁省大连市  116011
  • 出版日期:2010-08-06 发布日期:2010-08-06
  • 通讯作者: 刘晶，博士，教授，大连医科大学附属第一医院中英再生医学应用研究中心，辽宁省大连市 116011
  • 作者简介:诸葛栋★，男，1984年生，山东省临沂市人，汉族，大连医科大学在读硕士，主要从事成体干细胞神经向诱导分化方面的研究。 zhugedong@ sohu.com
  • 基金资助:

    大连市科技局国际合作项目(2009F11GH179)

Current research of differentiation from different sources-derived stem cells into neural cells

Zhuge Dong1, Liang Zhan-hua2, Song Lin1, Liu Jing1   

  1. 1 Center for Regenerative Medicine, 2 Department of Neurology, First Affiliated Hospital of Dalian Medical University, Dalian  116011, Liaoning Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Liu Jing, Doctor, Professor, Center for Regenerative Medicine, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
  • About author:Zhuge Dong★, Studying for master’s degree, Center for Regenerative Medicine, First Affiliated Hospital of Dalian Medical University; Dalian 116011, Liaoning Province, China zhugedong @ sohu.com
  • Supported by:

    the International Cooperation Program of Bureau of Science and Technology of Dalian City, No.2009F11GH179*

PDF

482

被引次数

13

摘要：

背景：神经系统变性病、脑卒中后遗症、颅脑及脊髓损伤等难治性疾病，多为大量神经元坏死、功能缺失。成年哺乳类动物中枢神经系统损伤后再生能力差，几乎无法产生新的神经元，完成自我修复。因此使损伤或变性的中枢神经组织恢复功能是人类多年来一直未能解决的治疗难题。干细胞的研究应用给神经系统疾病患者带来了希望。
目的：干细胞的研究已成为近年的研究热点，检索不同来源干细胞神经向分化的相关文献，对不同来源干细胞神经向分化能力进行比较。
方法：应用计算机检索Pubmed相关文献，英文检索词“neuronal differentiation”分别于“embryonic stem cells 、bone marrow mesenchymal stem cells、adipose derived mesenchymal stem cells、peripheral blood stem cells、umbilical cord blood ctem cells”组合检索。搜索以1995-01-01/2009-12-31相关文献，并限定文献语言种类为English。对资料进行初审，并查看每篇文献后的参考文献。筛选近年来不同来源干细胞神经向分化的所有文献。
结果与结论：理想的种子细胞应具有创伤小，易于获得，易于体外扩增，不涉及免疫排斥致瘤性及伦理学问题等。目前尚未发现具有以上优点的种子细胞。对于理想种子细胞不仅要考虑取材和法律问题更重要的细胞的定向分化能力。神经系统干细胞移植的种子细胞可以根据干细胞神经向分化能力的选择，由于目前对于各种干细胞神经向分化能力的研究存在很大差别，所以尚不能确定理想的种子细胞。未来的研究需要进一步研究不同来源干细胞的神经向分化能力。

关键词: 神经分化, 干细胞, 胚胎干细胞, 脐血干细胞, 骨髓间充质细胞, 外周血干细胞, 脂肪源性干细胞

Abstract:

BACKGROUND: Refractory nervous system diseases, including degenerative diseases, stroke sequelae, brain and spinal cord injury, mainly present a large number of neuronal necrosis and loss of function. Adult mammalian central nervous system regeneration after injury is poor, almost impossible to generate new neurons, or to complete self-repair. The recovery of function in injured or degenerated brain tissue has not been able to solve. Stem cell research and application offered hope for patients with nervous system disease.
OBJECTIVE: Studies on stem cells have become a hot point in recent years. We are in attempt to search the relevant literature of the differentiation from different sources-derived stem cells into neural cells, and to compare the ability of differentiation from different sources-derived stem cells into neural cells.
METHODS: We searched for the related articles in PubMed published from January 1995 and December 2009 in recent five years. The key words were “neuronal differentiation, embryonic stem cells, bone marrow mesenchymal stem cells. adipose derived mesenchymal stem cells, peripheral blood stem cells, umbilical cord blood stem cells” in English. The articles were selected primarily and the attached literatures were looked through. The articles were related to differentiation from different sources-derived stem cells into neural cells.
RESULTS AND CONCLUSION: Ideal seed cells should be minimally invasive, easy to access, easy-to-amplify in vitro, do not involve the immune rejection of tumorigenicity and ethical issues. These advantages have not yet found in the seed cells. The ideal seed cells derived not only to consider the legal issues and more importantly, directed differentiation of cells. Ideal cells should be selected according to the ability of stem cells during stem cell transplantation in the nervous system. There are great differences in research outcomes concerning the ability of differentiation of stem cells into neural cells, so ideal seed cells cannot be identified. Future research needs to study the differentiation of stem cells from different sources into neural cells.

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