中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (30): 5640-5643.doi: 10.3969/j.issn.1673-8225.2010.30.031

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

膝痹蠲汤干预膝关节创伤性滑膜炎兔关节液中白细胞介素1β和肿瘤坏死因子α的水平

张劲松   

  1. 湖南人文科技学院体育科学系，湖南省娄底市 417000
  • 出版日期:2010-07-23 发布日期:2010-07-23
  • 作者简介:张劲松★，男，1975年生，湖南省冷水江市人，汉族，2007年北京体育大学毕业，硕士，讲师，主要从事运动损伤防治的研究。 zhang_jinsong0402@126.com

Effects of xibijuan soup on interleukin 1 beta and tumor necrosis factor alpha levels in knee synovial fluid of rabbits with traumatic synovitis

Zhang Jin-song   

  1. Department of Sports Science, Hunan Institution of Humanities, Science and Technology, Loudi  417000, Hunan Province, China
  • Online:2010-07-23 Published:2010-07-23
  • About author:Zhang Jin-song★, Master, Department of Sports Science, Hunan Institution of Humanities, Science and Technology, Loudi 417000, Hunan Province, China zhang_jinsong0402@126.com

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摘要：

背景：已证实“膝痹蠲汤”治疗创伤性骨膜炎疗效确切，但以往研究多侧重于临床疗效观察。
目的：从炎症细胞因子角度了解中药“膝痹蠲汤”对兔膝关节创伤性滑膜炎关节液中白细胞介素和肿瘤坏死因子的影响，探讨其治疗膝关节创伤性滑膜炎的分子机制。
方法：将12只兔随机分为：模型组(肢)和中药组，其中模型组动物的正常肢设为对照组(肢)，共18个关节。应用电针刺激使膝关节重复被动进行单一屈伸动作造成膝关节滑膜损伤建立动物模型。在造模后第11天，用ELISA法检测各组兔膝关节液中白细胞介素1β和肿瘤坏死因子α水平情况。
结果与结论：模型组白细胞介素1β和肿瘤坏死因子α较对照组高(P < 0.05)；中药组白细胞介素1β和肿瘤坏死因子α比模型组低(P < 0.05)。结果提示，膝关节创伤性滑膜炎的病理过程与炎症细胞因子白细胞介素1β和肿瘤坏死因子α的水平密切相关。中药“膝痹蠲汤”能有效抑制炎症细胞因子，在分子水平上揭示了中药治疗膝关节创伤性滑膜炎的疗效机制。 

关键词: 滑膜炎, 白细胞介素1&beta, 肿瘤坏死因子&alpha, 中药, 膝关节, 机制

Abstract:

BACKGROUND: It has been confirmed that xibijuan soup received notable effectiveness in treating traumatic synovitis, however, most of Precious studies are focused on clinical outcomes.
OBJECTIVE: To understand the effects of xibijuan soup on interleukin 1 and tumor necrosis factor α (TNF-α) levels in knee synovial fluid of rabbit with traumatic synovitis, and to explore its molecular mechanism.
METHODS: Totally of 12 rabbits were randomly divided into model group (limbs) and the traditional Chinese medicine group. The healthy limbs of the model group were served as control group (limb). A total of 18 joints were included. Animals were prepared for models of knee joint synovial damage by stimulating knee joint to redundantly perform single flexion and extension movements. The expression of interleukin 1β and TNF-α in rabbit knee joint fluid was detected by ELISA method. 
RESULTS AND CONCLUSION: The levels of interleukin 1β and TNF-α in the model group were greater than those in the control group (P < 0.05). The levels of interleukin 1β and TNF-α were smaller in the traditional Chinese medicine group compared with the model group (P < 0.05). All results revealed that, the pathological process of knee traumatic synovitis is closely associated with levels of inflammatory cytokines IL-1β and TNF-α. The traditional Chinese medicine xibijuan soup can effectively inhibit the inflammatory cytokines, which revealed the therapeutic mechanism of traditional Chinese medicine on traumatic synovitis at the molecular level.

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