中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4461-4464.doi: 10.3969/j.issn.1673-8225.2011.24.022

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

高糖状态下小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白的表达

王晓林,甘  华,杜晓刚   

  1. 重庆医科大学附属第一医院肾脏内科,重庆市 400016
  • 收稿日期:2011-01-20 修回日期:2011-05-16 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 甘华,博士,教授,重庆医科大学附属第一医院肾脏内科,重庆市 400016 cqmusn4@yahoo.com.cn
  • 作者简介:王晓林★,女,1980年生,河南省人, 2011年重庆医科大学毕业,硕士,医师。主要从事肾小球疾病方面的研究。 guowangxia0lin@163.com
  • 基金资助:

    课题受高等学校博士学科点专项科研基金(20070631008)资助,S抑制性COP1介导的p53泛素化降解防治糖尿病肾病大鼠肾小球足细胞凋亡。

High glucose induces podocyte apoptosis and constitutive photomorphogenic 1 expression changes

Wang Xiao-lin, Gan Hua, Du Xiao-gang   

  1. Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2011-01-20 Revised:2011-05-16 Online:2011-06-11 Published:2011-06-11
  • Contact: Gan Hua, Doctor, Professor, Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China cqmusn4@yahoo.com.cn
  • About author:Wang Xiao-lin★, Master, Physician, Department of Nephrology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China guowangxia0lin@163.com
  • Supported by:

    the Scientific Research Foundation of Doctors in High Institutes, No. 20070631008*

摘要:

背景:组成型光形态建成1蛋白与细胞凋亡有关。
目的:观察高糖对体外培养的小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白表达的影响。
方法:将不同浓度葡萄糖溶液分别加入体外培养的条件永生性小鼠肾小球足细胞株培养液中,培养不同时间后检测肾小球足细胞凋亡指数和死亡指数,以筛选最佳剂量效应葡萄糖浓度。用30 mmol/L葡萄糖溶液干预小鼠肾小球足细胞(高糖组),并设立对照组和采用30 mmol/L甘露醇干预的甘露醇组。
结果与结论:在一定浓度范围内,葡萄糖呈时间和剂量依赖性诱导肾小球足细胞凋亡和死亡(P < 0.05),随着葡萄糖浓度的进一步加大,肾小球足细胞死亡指数明显升高,而凋亡指数变化不大。与对照组比较,高糖组凋亡指数显著增加(P < 0.05),其COP1 mRNA及蛋白表达水平均明显下降(P < 0.05)。 结果证实,高糖可诱导肾小球足细胞的凋亡,其机制可能与其下调COP1的表达有关。

关键词: 肾小球足细胞, 高糖, 组成型光形态建成1蛋白, 凋亡, 组织构建

Abstract:

BACKGROUND: Studies have shown that constitutive photomorphogenic 1 (COP1) is involved in cell apoptosis.
OBJECTIVE: To observe the effects of high glucose exposure on expression of in vitro cultured podocyte apoptosis and COP1 expression.
METHODS: Mouse podocytes of an immortalized cell line were treated with different concentrations of glucose. Apoptosis index and mortality index at different culture time were evaluated by flow cytometry to screen best dose-effect glucose concentration (30 mmol/L), which was used to treat high-glucose group. Control group and mannitol group (30 mmol/L) were set.
RESULTS AND CONCLUSION: Glucose induced podocyte apoptosis and death in time- and dose-dependent manner in certain concentration (P < 0.05). With increasing glucose concentration, mortality index was significantly elevated, but apoptosis index remained unchanged. Compared with control group, apoptosis index was significantly increased in high glucose group (P < 0.05), but COP1 mRNA and protein expression was significantly decreased (P < 0.05). Results showed that changes in COP1 expression may play a critical role in pathogenesis of high glucose induced podocyte apoptosis.

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