中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4457-4460.doi: 10.3969/j.issn.1673-8225.2011.24.021

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

人参皂苷Rd干预体外培养肝储脂细胞胶原的表达

谯时文1，王继丰2，黄  勤1，朱宗贵1，张知贵1   

  1. 1乐山职业技术学院护理系解剖教研室，四川省乐山市 614000
    2泸州医学院解剖学教研室，四川省泸州市 646000
  • 收稿日期:2010-12-11 修回日期:2010-12-23 出版日期:2011-06-11 发布日期:2011-06-11
  • 作者简介:谯时文，男，1964年生，四川省乐山市人，副教授，主要从事肝脏疾病机制的研究。 ch19711109@ 126.com

Effect of ginsenoside Rd on collagen expression in liver fat-storing cells

Qiao Shi-wen1, Wang Ji-feng2, Huang Qin1, Zhu Zong-gui1, Zhang Zhi-gui1   

  1. 1Department of Anatomy, Leshan Vocational and Technical College Leshan  614000, Sichuan Province, China
    2Department of Anatomy, Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Received:2010-12-11 Revised:2010-12-23 Online:2011-06-11 Published:2011-06-11
  • About author:Qiao Shi-wen, Associate professor, Department of Anatomy, Leshan Vocational and Technical College Leshan 614000, Sichuan Province, China ch19711109@126. com

PDF

279

被引次数

3

摘要：

背景：肝储脂细胞产生胶原是导致肝硬化的直接原因，调控肝储脂细胞产生胶原的能力即可以防治肝硬化。
目的：观察人参皂苷Rd对体外培养肝储脂细胞Ⅰ，Ⅲ型胶原表达的影响。
方法：体外培养肝储脂细胞，随机分为对照组和实验组，实验组用100 mg/L人参皂苷Rd进行培养干扰，用免疫组织化学、免疫荧光技术检测Ⅰ，Ⅲ型胶原基因的表达。
结果与结论：实验组比对照组，Ⅰ，Ⅲ型胶原阳性产物吸光度和面积比值明显降低(P < 0.05)，说明人参皂苷Rd对体外培养肝储脂细胞Ⅰ，Ⅲ型胶原的表达有明显调节作用，影响肝储脂细胞产生胶原纤维。

关键词: 人参皂苷, 肝, 储脂细胞, 胶原, 免疫组织化学, 免疫荧光

Abstract:

BACKGROUND: Collagens produced by liver fat-storing cells are the directly reasons for cirrhosis, thus, regulating capacity of fat-storing cells producing collagens can prevent cirrhosis.  
OBJECTIVE: To explore effect of ginsenoside Rd on expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro.
METHODS: The liver fat-storing cells were cultured in vitro and interfered with ginsenoside Rd. The expression of type Ⅰ and Ⅲ collagens was detected by immunohistochemistry and immunofluorescent.
RESULTS AND CONCLUSION: Compared with the control group, the positive/negative-area and the absorbance of the experimental group were obviously decreased (P < 0.05), suggesting that ginsenoside Rd regulates the expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro, which affects liver fat-storing cells producing collagens.

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