中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4525-4529.doi: 10.3969/j.issn.1673-8225.2011.24.039

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

组织蛋白酶L/G对创伤性深静脉血栓模型大鼠静脉壁的影响

李  文1，胡继红2，李兴国2，李宏昆2，章玉冰2，赵学凌2，王  兵2   

  1. 1云南省人民医院心内科，云南省昆明市  650032
    2昆明医学院第一附属医院骨科，云南省昆明市  650032
  • 收稿日期:2011-02-25 修回日期:2011-05-11 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 王兵，博士，副主任医师，昆明医学院第一附属医院骨科，云南省昆明市 650032
  • 作者简介:李文，女，1969年生，云南省昆明市人，汉族，1990年昆明医学院毕业，副主任医师，主要从事临床超声影像方面的研究。 并列第一作者：胡继红，男，1967年生，云南省昆明市人，汉族，昆明医学院在读博士，副教授，主要从事影像及放射介入方面的研究。
  • 基金资助:

    国家自然科学基金资助项目 (30960389，81060151)；云南省科技厅-昆明医学院联合项目(2009cd159)。

Effect of cathepsin L/G on venous vascular wall in traumatic deep vein thrombosis rat models

Li Wen1, Hu Ji-hong2, Li Xing-guo2, Li Hong-kun2, Zhang Yu-bing2, Zhao Xue-ling2, Wang Bing2   

  1. 1Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming  650032, Yunnan Province, China
    2Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming  650032, Yunnan Province, China
  • Received:2011-02-25 Revised:2011-05-11 Online:2011-06-11 Published:2011-06-11
  • Contact: Wang Bing, Doctor, Associate chief physician, Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China wbdoctor@hotmail.com
  • About author:Li Wen, Associate chief physician, Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming 650032, Yunnan Province, China akenika@hotmail.com Hu Ji-hong, Studying for doctorate, Associate professor, Department of Orthopaedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China akenika168@gmail.com Li Wen and Hu Jing-hong contributed equally to this article.
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*, 81060151*; the Joint Funds of Yunnan Sci-Tech Department and Kunming Medical College, No. 2009cd159*

PDF

511

被引次数

9

摘要：

背景：目前，深静脉血栓的分子病因学机制及其形成的核心调控网络仍未完全阐明，对于深静脉血栓的早期诊断预测也无理想的方法。
目的：研究组织蛋白酶L/G与创伤性深静脉血栓的预测。
方法：采用蚊式钳夹闭50只SD大鼠双侧股静脉的3个不同部位3 s随后予以模具制动制备大鼠创伤性深静脉血栓模型，根据股静脉血栓形成的不同阶段和生物学特征，将模型大鼠分为血栓形成前组、血栓形成组和无血栓形成组，另取10只正常大鼠作为对照组。在相应时间点取大鼠创伤静脉，提取总RNA，经过基因芯片技术筛选差异表达基因，并进一步应用real-time PCR进行验证。
结果与结论：基因芯片杂交结果发现组织蛋白酶L/G基因在各组间差异表达明显，其中血栓形成组最高，无血栓形成组和血栓形成前组次之，均明显高于对照组(P < 0.05)；real-time PCR分析结果与基因芯片杂交分析结果相一致。说明局部静脉血管壁中组织蛋白酶L/G表达水平升高与创伤性深静脉血栓形成有关，可作为深静脉血栓形成早期诊断、预测的候选分子标志。

关键词: 组织蛋白酶L, 组织蛋白酶G, 基因, 候选分子, 创伤性深静脉血栓, 静脉壁

Abstract:

BACKGROUND: At present, the basic molecular etiological mechanism and core regulatory network of deep vein thrombosis (DVT) remains uncertain, and there is not an ideal measure for early diagnosis of DVT.
OBJECTIVE: To study the underlying impact of cathepsin L/G in DVT rat model.
METHODS: DVT rat models (n = 50) were established by clamping both femoral vein in three different positions within 3 seconds with mosquito forceps and fixing with cast. According to different observation phases and biological situations of the femoral vein thrombosis, model rats were divided into thrombogenesis group, pre-thrombogenesis group and non-thrombogenesis group. An additional 10 normal rats served as control group. Femoral vein was obtained at corresponding time points to exact total RNA. After a gene chip-based screening, the data of gene expression were further dissected by real-time PCR. 
RESULTS AND CONCLUSUON: Gene chip hybridization analysis results demonstrated that differential expression of cathepsin L/G gene was significant among groups, and the expression was greatest in the thrombogenesis group, followed by pre-thrombogenesis and non-thrombogenesis groups, which was significantly greater than the control group (P < 0.05). Real-time PCR analysis results were consistent with gene chip hybridization analysis results. These indicate that DVT is associated with an increase in expression of cathepsin L/G in local venous vascular wall, and they may be candidate molecular markers for early diagnosis of deep vein thrombosis.

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