中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4380-4384.doi: 10.3969/j.issn.1673-8225.2010.24.003

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌孕激素联合对去卵巢大鼠骨密度和骨形态计量学的影响

梁  敏，张  劼，梁杏欢，罗佐杰   

  1. 广西医科大学第一附属医院内分泌科，广西壮族自治区南宁市 530021
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 作者简介:梁 敏☆，女，1972 年生，广西壮族自治区南宁市人，汉族，2003年中南大学湘雅医学院毕业，博士，教授，主要从事骨质疏松症研究。 liangmin7268@sohu.com
  • 基金资助:

    课题由广西自然科学基金资助(桂科青0447042)，项目名称：雌孕激素联合防治去卵巢骨质疏松模型大鼠的实验研究。

Effects of combining estrogen and progesterone on bone mineral density and histomorphometry in ovariectomized rats

Liang Min, Zhang Jie, Liang Xing-huan, Luo Zuo-jie   

  1. Department of Endocrinology, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Online:2010-06-11 Published:2010-06-11
  • About author:Liang Min☆, Doctor, Professor, Department of Endocrinology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China liangmin7268@sohu.com
  • Supported by:

     the Guangxi Natural Science Foundation, No. 0447042*

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511

被引次数

23

摘要：

背景：雌孕激素联合治疗绝经后骨质疏松症不仅能减轻雌激素的不良反应，而且能增强疗效，越来越引起关注。
目的：验证雌孕激素联合对去卵巢雌性SD大鼠骨密度和骨组织形态计量学的作用。
方法：雌性SD大鼠随机分为5组。假手术组仅行开关术；其余各组大鼠双侧卵巢切除。雌二醇组和雌二醇+左炔诺孕酮组术后给予17β–雌二醇10 μg/(kg•d) 灌饲，左炔诺孕酮组和雌二醇+左炔诺孕酮组术后给予18-甲左炔诺孕酮60 μg/(kg•d)灌饲，假手术组和卵巢切除组予等量生理盐水灌饲。实验12周后处死大鼠。采用DEXA骨密度仪测定全身骨密度，截取第4腰椎进行骨形态计量学静态参数和动态参数的测量，测定骨代谢生化指标。
结果与结论：卵巢切除组和左炔诺孕酮组全身骨密度、股骨质量低于假手术组、雌二醇组和雌二醇+左炔诺孕酮组 (P < 0.05)，雌二醇+左炔诺孕酮组和雌二醇组差异无显著性意义( P > 0.05)。与假手术组比较，卵巢切除组和左炔诺孕酮组骨小梁面积百分数、骨小梁厚度、骨小梁数量下降(P < 0.05)，骨小梁分离度增加(P < 0.05)，骨矿化沉积率、骨表面积骨形成率、骨体积骨形成率和骨组织体积骨形成率增加(P < 0.05)。与卵巢切除组相比，雌二醇组和雌二醇+左炔诺孕酮组骨小梁面积百分数、骨小梁厚度、骨小梁数量上升(P < 0.05)，骨小梁分离度降低(P < 0.05)，骨表面积骨形成率和骨体积骨形成率降低(P < 0.05)。雌二醇+左炔诺孕酮组和雌二醇组上述指标比较，均差异无显著性意义( P > 0.05)。结果证实，17β-雌二醇和左炔诺孕酮联合应用可增加骨密度，降低骨转换率，且无明显抑制骨形成作用。

关键词: 左炔诺孕酮, 17β-雌二醇, SD大鼠, 骨密度, 骨组织形态计量学

Abstract:

BACKGROUND: Treatment of menopausal osteoporosis using estrogen combined with progesterone not only build up the therapeutic effect, but also reduce the side effect of estrogen, thus attracting more and more attention.
OBJECTIVE: To observe the combining effects of 17β-estradiol and levonorgestrel on bone mineral density (BMD) and histomorphometry in ovariectomized SD rats.
METHODS: Female SD rats were randomly assigned to the sham-operated group (sham group), ovariectomized group (OVX group), 17β-estradiol group (EST group), levonorgestrel group (LNG group) and 17β-estradiol combined with levonorgestrel group (EST+LNG group). After ablating bilateral ovarian model, EST, and EST+LNG group rats were administrated 17β-estradiol (10 μg/kg per day), LNG group and EST+LNG group rats were administrated levonorgestrel(60 μg/kg per day). The rats were killed 12 weeks later. The BMD of whole body was measured by bone densitometry. The fourth lumbar vertebra sections were cut for bone histomorphometry analysis. The biochemical bone markers were measured.
RESULTS AND CONCLUSION: BMD and weight of thighbone in OVX group and LNG group were lower than of the sham, EST and EST+LNG groups (P < 0.05). But BMD and weight of thighbone had no significant difference between the EST group and EST+LNG group (P > 0.05). Compared with the sham group, the bone trabecular area percentage, thickness and number in OVX group and LNG group were decreased (P < 0.05), while the bone trabecular resolution, bone mineral apposition rate, bone surface area formation rate, bone volume formation rate and bone mineral apposition rate in OVX group and LNG group were increased
(P < 0.05). Compared with OVX group, the bone trabecular area percentage, thickness and number in EST group and EST+LNG group were increased (P < 0.05), while the bone trabecular resolution, bone surface area formation rate, bone volume formation rate in EST group and EST+LNG group were decreased (P < 0.05). The differences between the EST group and EST+LNG group had no significant differences (P > 0.05). All results demonstrated that combining 17β-estradiol with levonorgestrel can improve BMD and reverse the high bone turnover rate, which have no harm to bone formation.

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