中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2801-2804.doi: 10.3969/j.issn.1673-8225.2010.15.034

• 组织构建综述 tissue construction review • 上一篇    下一篇

血管内皮祖细胞在组织工程皮肤构建中的意义

刘海平1，汪  虹1，董晓华2，杨  华1   

  1. 1昆明医学院第二附属医院烧伤科，云南省烧伤研究中心，云南省昆明市  650000；2昆明医学院第一附属医院心内科，云南省昆明市 650000
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 汪 虹，教授，副所长，主任医师，硕士生导师，昆明医学院第二附属医院烧伤科，云南省烧伤研究中心，云南省昆明市 650000 whkunming631015@sina.com
  • 作者简介:刘海平★，男，1981年生，山西省朔州市人，汉族，昆明医学院在读硕士，主要从事组织工程皮肤方面的研究。 l.xp1982@163.com

Significance of endothelial progenitor cells for tissue engineered skin construction  

Liu Hai-ping1, Wang Hong1, Dong Xiao-hua2, Yang Hua1   

  1. 1 Department of Burns, Second Affiliated Hospital, Kunming Medical College, Burn Research Center of Yunnan Province, Kunming   650000, Yunnan Province, China; 2 Department of Cardiology, First Affiliated Hospital, Kunming Medical College, Kunming   650000, Yunnan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Wang Hong, Professor, Chief physician, Master’s supervisor, Department of Burns, Second Affiliated Hospital, Kunming Medical College, Burn Research Center of Yunnan Province, Kunming 650000, Yunnan Province, China whkunming631015@sina.com
  • About author:Liu Hai-ping★, Studying for master’s degree, Department of Burns, Second Affiliated Hospital, Kunming Medical College, Burn Research Center of Yunnan Province, Kunming 650000, Yunnan Province, China l.xp1982@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30760260*; the Foundation of Yunnan Scientific Plan, No. 2007C247M*

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被引次数

1

摘要：

背景：血管内皮祖细胞可加速缺血部位的血管化。
目的：综合分析血管内皮祖细胞加速组织工程皮肤血管化的方法和途径。
方法：计算机检索中国期刊全文数据及PubMed数据库1999-09/2009-09相关文章，检索词为“血管内皮祖细胞，组织工程皮肤，血管新生，endothelial progenitor cell(EPC)，tissue engineered skin，vascularization  ”。纳入与血管内皮祖细胞与组织工程皮肤研究现状与发展密切相关文章，包括：①血管内皮祖细胞的生物学特性、动员方法及促进血管新生的可能机制。②组织工程皮肤的应用现状。③组织工程皮肤中血管内皮祖细胞的研究现状。
结果与结论：血管内皮祖细胞是指特异性归巢于血管新生组织，并能分化增生为成熟内皮细胞的一群祖细胞。近年来大量的动物实验证实，通过移植体外培养的血管内皮祖细胞到肢体缺血部位，可以加速缺血部位的血管化。在心肌梗死方面已经进行了自体血管内皮祖细胞治疗心肌梗死的临床试验，使其临床应用逐渐成为可能。而目前构建的组织工程皮肤中缺少血管成分，严重影响人工皮肤中活细胞的存活及其功能实现，如何构建出含有血管的人工皮肤及加速人工真皮替代物血管化引起了广大的关注。应用血管内皮祖细胞促进组织工程皮肤血管化进而提高其成活率有重要的临床意义。

关键词: 血管内皮祖细胞, 组织工程皮肤, 血管新生, 组织工程, 综述文献

Abstract:

BACKGROUND: Vascular endothelial progenitor cells can accumulate the vascularization in ischemic region.
OBJECTIVE: To analyze how to accelerate tissue engineered skin vascularization by endothelial progenitor cells.
METHODS: A computer-based online search was conducted in China Academic Journals Database and PubMed with the key words of “endothelial progenitor cell (EPC), tissue engineered skin, vascularization” in both English and Chinese from September 1999 to September 2009. Inclusion criteria: Articles were highly correlated with endothelial progenitor cells and tissue engineered skin development, including biological characteristics, stimulating methods, and mechanism of promoting vascular production of endothelial progenitor cells; current situation of tissue engineered skin; current situation of endothelial progenitor cells for tissue engineered skin construction.
RESULTS AND CONCLUSION: The endothelial progenitor cells belonged to a crowd of cells which specifically homed on vascular cambium, as well as differentiated and proliferated into mature endothelial cells. Nowadays, a lot of animal tests proved that it could accelerate the vascularization in ischemic region by transplanting the in vitro cultured endothelial progenitor cells into the ischemic region. The clinical application would be true when auto-endothelial progenitor cells have been successfully used to treat myocardial infarction. However, the tissue engineered skin was in defect of vessel, which seriously influenced the cell survival of human skin and the functional achievement. Therefore, how to construct artificial skin which was rich in vessels and how to accelerate the human skin substitution vascularization have attracted more attention. It is of significance for endothelial progenitor cells to promote tissue engineered skin vascularization so as to increase the survival rate.

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